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  4. Recombinant Human Hematopoietic prostaglandin D synthase (HPGDS)

Recombinant Human Hematopoietic prostaglandin D synthase (HPGDS)

  • 货号：
    CSB-YP017852HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP017852HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP017852HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP017852HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP017852HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    HPGDS
  • Uniprot No.：
  • 别名：
    Cerebrin 28; Glutathione dependent PGD synthetase; Glutathione S-transferase; Glutathione-dependent PGD synthase; Glutathione-requiring prostaglandin D synthase; GST class-sigma; H PGDS; H-PGDS; Haematopoietic prostaglandin D synthase; Haematopoietic prostaglandin D2 synthase; Hematopoietic prostaglandin D synthase; Hpgds; HPGDS_HUMAN; Prostaglandin D synthase; Prostaglandin D2 synthase hematopoietic; Prostaglandin H2 D isomerase; Prostaglandin-H2 D-isomerase; PTGDS2
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-199
  • 氨基酸序列
    MPNYKLTYFN MRGRAEIIRY IFAYLDIQYE DHRIEQADWP EIKSTLPFGK IPILEVDGLT LHQSLAIARY LTKNTDLAGN TEMEQCHVDA IVDTLDDFMS CFPWAEKKQD VKEQMFNELL TYNAPHLMQD LDTYLGGREW LIGNSVTWAD FYWEICSTTL LVFKPDLLDN HPRLVTLRKK VQAIPAVANW IKRRPQTKL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Bifunctional enzyme which catalyzes both the conversion of PGH2 to PGD2, a prostaglandin involved in smooth muscle contraction/relaxation and a potent inhibitor of platelet aggregation, and the conjugation of glutathione with a wide range of aryl halides and organic isothiocyanates. Also exhibits low glutathione-peroxidase activity towards cumene hydroperoxide.
  • 基因功能参考文献：
    1. we have utilized a broad-scaled affinity proteomics approach to identify three proteins (CCL5, HPGDS, and NPSR1) with altered plasma levels in asthmatic children compared to healthy controls, representing the first evaluation of HPGDS and NPSR1 in plasma. PMID: 27145233
    2. the development of a competition binding assay amenable to high-throughput screening (HTS) in a scintillation proximity assay (SPA) format. This assay was used to screen an in-house compound library of approximately 280,000 compounds for novel H-PGDS inhibitors. PMID: 27485270
    3. The effect of C. molle alkaloid extract on H-PGDS was studied. The alkaloid extract exhibited a 70% inhibition on H-PGDS with an IC50 of 13.7 mug/ml. PMID: 24996417
    4. the Val187Ile HPGDS isoenzyme common among African Americans is not associated with colorectal cancer risk PMID: 21821144
    5. COX-1 and H-PGDS were rapidly ubiquitinated and degraded through the ubiquitin-proteasome system in response to the elevation of the intracellular calcium level PMID: 22049022
    6. In patients with mixed atrophy, testicular mast cell numbers and phenotypes change with respect to the ability to express COX2 and PGDS-H. PMID: 21683347
    7. Results describe the activation by Mg++ of human hematopoietic prostaglandin D(2) synthase, using Raman spectroscopy. PMID: 20074808
    8. New polymorphisms of haematopoietic prostaglandin D synthase PMID: 12002745
    9. crystal structure when bound to glutathione and Ca+ or Mg+, and enzyme activation PMID: 12627223
    10. Data suggest that the suppressive effect of hematopoietic prostaglandin D synthase on lung injury could be partly mediated by edema formation and inhibition of genes involved in fibrosis. PMID: 12707014
    11. X-ray structure of human H-PGDS complexed with an inhibitor, 2-(2'-benzothiazolyl)-5-styryl-3-(4'-phthalhydrazidyl) tetrazolium chloride (BSPT) at 1.9 A resolution in the presence of Mg(2+). PMID: 15113825
    12. single nucleotide polymorphism association with asthma in children in Japan PMID: 18946232
    13. PU.1 cooperates with MAPK-activated cJun to maximally induce L-PGDS expression in macrophages. PMID: 19181746

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    GST superfamily, Sigma family
  • 组织特异性：
    Expressed in a number of megakaryocytic cell lines but not in platelets. Highly expressed in adipose tissue, macrophages and placenta. Also expressed at lower levels in lung, heart, lymph nodes, appendix, bone marrow and fetal liver.
  • 数据库链接：

    HGNC: 17890

    OMIM: 602598

    KEGG: hsa:27306

    STRING: 9606.ENSP00000295256

    UniGene: Hs.128433