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  4. Recombinant Human Hemoglobin subunit epsilon (HBE1)

Recombinant Human Hemoglobin subunit epsilon (HBE1)

  • 中文名称：
    人HBE1重组蛋白
  • 货号：
    CSB-YP010153HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人HBE1重组蛋白
  • 货号：
    CSB-EP010153HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人HBE1重组蛋白
  • 货号：
    CSB-EP010153HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人HBE1重组蛋白
  • 货号：
    CSB-BP010153HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人HBE1重组蛋白
  • 货号：
    CSB-MP010153HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Epsilon-globin; HBE_HUMAN; HBE1; Hemoglobin epsilon chain; Hemoglobin subunit epsilon
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-147
  • 氨基酸序列
    VHFTAEEKA AVTSLWSKMN VEEAGGEALG RLLVVYPWTQ RFFDSFGNLS SPSAILGNPK VKAHGKKVLT SFGDAIKNMD NLKPAFAKLS ELHCDKLHVD PENFKLLGNV MVIILATHFG KEFTPEVQAA WQKLVSAVAI ALAHKYH
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    The epsilon chain is a beta-type chain of early mammalian embryonic hemoglobin.
  • 基因功能参考文献：
    1. epsilon-globin expression is regulated by SUV4-20h1. PMID: 26802048
    2. These data indicate that HP1-gamma is a novel epigenetic repressor of epsilon-globin gene expression. PMID: 28154185
    3. Fetal varepsilon-globin gene expression is significantly greater than adult expression and is increased in maternal plasma compared to non-pregnant samples. PMID: 27002261
    4. findings indicate the Xmn1-Ggamma polymorphism is likely to be one factor that influences the production of HbF in beta-thalassemia/HbE patients, but not in homozygous HbE patients PMID: 25043956
    5. Data indicate that Myb and BCL11A cooperate with DNMT1 to achieve developmental repression of embryonic and fetal beta-like human transgenic globin genes in the adult erythroid environment. PMID: 24371119
    6. Data indicate that in embryonic stem cells (hESCs)-derived erythroblasts where both epsilon and gamma globin were active, epsilon globin was immediately silenced upon transfer, whereas gamma globin continued to be expressed for months. PMID: 23993951
    7. A plasma proteome analysis is performed to identify differentially expressed proteins compared between normal subjects and patients with mild and severe forms of beta-thalassemia/hemoglobin E (Hb E). PMID: 23161390
    8. In the present study, involving the characterization of mutations in transfusion-dependent thalassemia in the Gwalior-Chambal region of Central India, Hb E [beta26(B8)Glu-->Lys, GAG>AAG] was found in high frequency. PMID: 22738610
    9. the observed in vivo RBC mild oxidative stress arises, at least in part, from the molecular consequences of the HbE mutation. PMID: 22260787
    10. investigation of interaction of HbE w/ other abnormal hemoglobins found in India isolated by cation exchange chromatography; interactions complicate Hb isolation and thus diagnosis of hemoglobinopathies/beta-thalassemias in heterozygous patients PMID: 21986214
    11. Erythroblasts from beta-thalassemia/Hb E patients only show activation of the unfolded protein response pathway in response to internal stress, whereas normal erythroblasts respond to both internal and external stress. PMID: 20015891
    12. patients with hemoglobin Ebeta thalassemia have a significant decrease in the oxygen affinity of their hemoglobin, that is an increased P(50) value, in response to anemia. PMID: 20833979
    13. The frequency of the Ggamma-158(C-->T) polymorphism was relatively high in Southern Chinese patients with HbE/beta-thalassemia major. PMID: 20822527
    14. Crystallization and X-ray structure of this protein, isolated from blood of beta-thalassemic patients PMID: 12659864
    15. HbE carrier status has been shown to confer protection against Plasmodium falciparum malaria PMID: 15114532
    16. Genomic and phylogenetic footprinting at the epsilon-globin silencer region in human cells PMID: 15157738
    17. The crystal structure of HbE at 1.74 angstrom resolution in R2 state is used to provide probable explanations of the thermal stability and instability of HbE. PMID: 15449937
    18. Mutation screening identified no sequence variations apart from the expected 5'epsilon /HincII polymorphism, suggesting that genomic alterations in the HBE1 gene are most likely incompatible with normal erythropoiesis and proper embryonic development PMID: 16432873
    19. results suggest in the context of the whole beta-globin locus, other proximal & upstream epsilon promoter elements & competition by downstream globin genes contribute to silencing of epsilon-globin in cells of definitive erythropoiesis [epsilon-globin] PMID: 16514181
    20. We discussed these results in the context of intergenic transcription and chromatin opening in the beta-globin gene cluster. PMID: 16620781
    21. The epsilon-globin mRNA is modestly unstable in immature, transcriptionally active erythroid cells, but that this characteristic has relatively little impact on the accumulation of epsilon-globin mRNA at subsequent stages of terminal differentiation. PMID: 17003365
    22. The epsilon-globin gene differs in its distance sensitivity to the locus control region from the other beta-like globin genes, which is, at least in part, determined by the transcription factor EKLF. PMID: 17548470
    23. the HbA/HbE polymorphism would not be a major genetic factor influencing the onset of cerebral malaria in Thailand. PMID: 18787944
    24. The slightly increased levels of Hb A(2), 3.5% +/- 0.4%, which is shown in the carriers of Hb E, confirm that Hb E is the silent phenotype of beta(+)-thalassemia. PMID: 18940720
    25. It is necessary to perform a comprehensive DNA analysis for alpha-thalassemia in cases of homozygous HbE when their partner is suspected of having alpha-thalassemia 1 gene. PMID: 19323016

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  • 蛋白家族：
    Globin family
  • 组织特异性：
    Red blood cells.
  • 数据库链接：

    HGNC: 4830

    OMIM: 142100

    KEGG: hsa:3046

    STRING: 9606.ENSP00000292896

    UniGene: Hs.655195