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  4. Recombinant Human Hemoglobin subunit gamma-1 (HBG1)

Recombinant Human Hemoglobin subunit gamma-1 (HBG1)

  • 中文名称：
    人HBG1重组蛋白
  • 货号：
    CSB-YP010155HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人HBG1重组蛋白
  • 货号：
    CSB-EP010155HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人HBG1重组蛋白
  • 货号：
    CSB-BP010155HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人HBG1重组蛋白
  • 货号：
    CSB-MP010155HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    HBG1; PRO2979; Hemoglobin subunit gamma-1; Gamma-1-globin; Hb F Agamma; Hemoglobin gamma-1 chain; Hemoglobin gamma-A chain
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-147
  • 氨基酸序列
    GHFTEEDKA TITSLWGKVN VEDAGGETLG RLLVVYPWTQ RFFDSFGNLS SASAIMGNPK VKAHGKKVLT SLGDAIKHLD DLKGTFAQLS ELHCDKLHVD PENFKLLGNV LVTVLAIHFG KEFTPEVQAS WQKMVTAVAS ALSSRYH
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Gamma chains make up the fetal hemoglobin F, in combination with alpha chains.
  • 基因功能参考文献：
    1. Data suggest that studying genotype frequency of the Xmn1 gammaG globin polymorphism (-158C>T ) in Siwa Oasis, Egypt can be considered as a starting point for further research targeting this community sector. PMID: 29932071
    2. A significant relationship between two variations (+25G/A and -499T/A) in Agamma gene promotor and fetal hemoglobin levels was observed. PMID: 29412791
    3. study describes the characterization of the rs368698783 (+25 G->A) polymorphism of the Agamma-globin gene associated in beta(0)39 thalassemia patients with high HbF in erythroid precursor cells PMID: 28851297
    4. Introduction of the British HPFH mutation into the fetal globin promoter in a human cell model causes elevated fetal globin expression. The British HPFH mutation creates a de novo binding site both in vitro and in vivo for the potent erythroid activator KLF1. PMID: 28659276
    5. A Ly1 antibody reactive (LYAR)-binding motif disruptive regulatory single-nucleotide polymorphisms rs368698783 (G/A) from LD block 5 in the proximal promoter of hemoglobin subunit gamma 1 (HBG1) was found to be a significant predictor for beta-thalassemia clinical severity. PMID: 28669403
    6. Study found a novel polymorphism of the A-gamma-globin gene in four families with b0-thalassemia and high levels of HbF expression. Additionally, evidence suggesting that the Ac-globin gene +25(G-A) polymorphism decreases the efficiency of the interaction between this sequence and specific DNA binding protein complexes. PMID: 26897028
    7. These data confirm the regulatory role of the HBG1: g.-225_-222 region that exerts its effect under conditions of erythropoietic stress characteristic for beta-thal patients. PMID: 26575252
    8. Data show that the ancestral allele C at rs2855126, located upstream of gamma-globin (HBG1) is associated with increased serum uric acid levels. PMID: 26686224
    9. analysis of a point mutation that increases fetal globin expression through de novo recruitment of the activator TAL1 to promote chromatin looping of distal enhancers to the modified gamma-globin promoter PMID: 25971621
    10. a successful induction of gamma-globin includes a reduction in BCL11A, KLF1 and TAL1 expression. PMID: 26053062
    11. This report we discusses the molecular characteristics and diagnostic criteria of a new (A)gamma chain variant that was detected during newborn screening and named Hb F-Sykesville MD [(A)gamma113(G15)Val --> Ile; HBG1: c.340G>A]. PMID: 25565447
    12. Results indicate that compound II induces the gamma globin gene in hydroxyurea (HU)-resistant primary adult erythroid cells and suggest potenetial therapy for sickle cell disease (SCD) and severe beta-thalassemias. PMID: 25986606
    13. Inactivation of HDAC1 or HDAC2 induces gamma-globin expression without altering cell cycle or proliferation. PMID: 25808664
    14. There is synergism between developmental stage-specific recruitments of the ATF2 protein complex and expression of gamma-globin during erythropoiesis. PMID: 24223142
    15. data are consistent with a model in which WDR5 binds the gamma-globin promoter in a PRMT5-dependent manner. PMID: 22689669
    16. the stimulation of GPCRs supports the postulated connection between cAMP/PKA and NO/cGMP pathways in activation of gamma-globin expression, via JUN and p38 MAPK signaling. PMID: 23425329
    17. Methylation sites 28, 122, 231 and 234 bp of gamma-globin gene promoter are found both in patients with beta-thalassemia major and healthy adults. PMID: 22739173
    18. NF-Y recruits the developmentally regulated, erythroid transcription activator GATA-2 and general repressor BCL11A to modulate transcription of the gamma-globin gene. PMID: 23071749
    19. Activation of the p38 MAPK pathway by sodium butyrate augments gamma-globin expression through a CREB1 response element (CRE) that is present in the upstream promoter region of Ggamma gene. PMID: 22469229
    20. Three different gene rearrangements in three unrelated patients with the same breakpoints in the gamma-globin gene can lead to different levels of Hb A2 depending on the remaining number of gamma-globin genes. PMID: 22273484
    21. results establish SATB2 as a novel gamma-globin gene regulator and provide a glimpse of the differential and cooperative roles of SATB family proteins in modulating clustered genes transcription PMID: 22825848
    22. Alternative NLI complexes mediate gamma-globin transcription or silencing through long-range locus control region interactions involving an intergenic site of noncoding RNA transcription and that ETO2 is critical to this process. PMID: 22010104
    23. Low-dose hydroxyurea combined with sodium butyrate can up-regulate gamma globin gene expression in human erythroid progenitor cells. PMID: 19861270
    24. Sodium butyrate increases the level of acetylated histone in gamma-globin gene promoter regions. PMID: 20584642
    25. Xmn I polymorphism associated with concomitant activation of Ggamma and Agamma globin gene transcription on a beta0-thalassemia chromosome. PMID: 21144779
    26. polymorphisms -396_-391 del HBG2, -369 SNP HBG2 and -271 SNP HBG1 correlated with HbF levels, hence, it suggests an important role of HBG2 and HBG1 gene polymorphisms on the HbF synthesis. PMID: 20602015
    27. role of the hematopoietic transcription factor GATA-1, its cofactor FOG-1, and the associated chromatin remodeling complex NuRD in the developmental silencing of HBG1 and HBG2 gene expression PMID: 20439494
    28. Disrupting the bindings of the Oct-1 transcriptional factors with the decoy oligonucleotide provides a novel approach for inducing expression of the gamma-globin genes. PMID: 19327156
    29. the A allele of -588, [+] allele of XmnI and HS-111 (-21 A) variation are useful genetic markers to differentiate between beta-thalassemia major and beta-thalassemia intermedia patients PMID: 19958188
    30. the gamma-globin -195 mutation is the unique cause of elevation of Hb F in Brazilian hereditary persistence of fetal hemoglobin PMID: 19958189
    31. Developmental specificity of recruitment of TBP to the TATA box of the human gamma-globin gene PMID: 11960008
    32. DRED binds with high affinity to DR1 sites in the human epsilon & gamma globin promoters, but the adult beta-globin promoter has no DR1 element. An HPFH mutation in a DR1 site causes elevated gamma-globin transcription & reduces TR2/TR4 binding in vitro. PMID: 12093744
    33. Apicidin activates the A gamma globin gene promoter. Activation of the Agamma-globin promoter by apicidin could be inhibited by p38 inhibitor SB203580 PMID: 12393499
    34. In transgenic mice treated with short-chain fatty acid derivatives once daily for 5 days, human gamma globin mRNA increased 2-fold, reticulocytes increased 2-fold. PMID: 12393583
    35. human gamma-globin gene expression is developmentally regulated by the CCAAT box PMID: 14645237
    36. Direct repeat element in the promoter region of the gamma-globin gene autonomously mediates definitive stage-specific gene silencing. PMID: 15831451
    37. Together, these results show that the cAMP pathway blocks gamma-globin gene expression in K562 cells by increasing c-Myb expression. PMID: 16631597
    38. A novel gamma-globin-inducing short-chain fatty acid derivative (SCFAD), RB7, which was identified through computational modeling, produced a 6-fold induction in a reporter assay. PMID: 16849648
    39. These results suggest that different PKC isoforms may exert ontogenetic-specific functions in erythropoiesis and that modulation of PKCalpha might affect the activity of (A)gamma-promoter-driven reporters. PMID: 17212360
    40. data provide important clues for identifying and validating trans-activators that activate the gamma-globin gene in fetuses, and trans-acting factors involved in silencing the gamma-globin gene in adults PMID: 17612629
    41. analysis of a model for dynamic post-transcriptional control of gamma-globin gene expression, through modulation of the stability of its encoding mRNA PMID: 17976188
    42. study reports 2 new forms of nondeletional hereditary persistence of fetal hemoglobin; the presence of a (G)gamma-196 C-->T in the first case and an (A)gamma-201 C-->T in the second was revealed PMID: 18096417
    43. during definitive erythropoiesis, gamma-globin gene expression is silenced, in part, by binding a protein complex containing GATA-1, FOG-1, and Mi2 at the -566/-567 GATA sites of the proximal gamma-globin promoters PMID: 18347053
    44. Very low HBA2 levels of HBA2 in compound heterozygotes result from functional inhibition of the HBD gene in cis to the HBG1 gene bearing the nd-HPFH mutation. Absence of the HBG1:g.-225-222AGCAdel variation correlated with lower HbF & higher HbA2 levels. PMID: 18615450
    45. Observations from these two unique cases provide solid evidence that the Alphagamma - 158 C > T mutation plays an important role in Agamma-globin gene transcription. PMID: 18718799
    46. EKLF and the co-activator BRG1 are co-opted by short-chain fatty acid derivatives to activate the gamma globin genes PMID: 19220418
    47. The data suggest that miR-210 might be involved in increased expression of gamma-globin genes in differentiating erythroid cells. PMID: 19712585

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  • 蛋白家族：
    Globin family
  • 组织特异性：
    Red blood cells.
  • 数据库链接：

    HGNC: 4831

    OMIM: 142200

    KEGG: hsa:3047

    UniGene: Hs.702189