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  4. Recombinant Human Histidine triad nucleotide-binding protein 1 (HINT1)

Recombinant Human Histidine triad nucleotide-binding protein 1 (HINT1)

  • 货号：
    CSB-YP343812HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP343812HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP343812HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP343812HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP343812HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    HINT1
  • Uniprot No.：
  • 别名：
    Adenosine 5' monophosphoramidase; Adenosine 5''-monophosphoramidase; HINT 1; HINT1; HINT1_HUMAN; Histidine triad nucleotide binding protein 1; Histidine triad nucleotide-binding protein 1; PKCI 1; PKCI-1; PKCI1; PRKCNH1; Protein kinase C inhibitor 1; Protein kinase C interacting protein 1; Protein kinase C-interacting protein 1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-126
  • 氨基酸序列
    ADEIAKAQV ARPGGDTIFG KIIRKEIPAK IIFEDDRCLA FHDISPQAPT HFLVIPKKHI SQISVAEDDD ESLLGHLMIV GKKCAADLGL NKGYRMVVNE GSDGGQSVYH VHLHVLGGRQ MHWPPG
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Exhibits adenosine 5'-monophosphoramidase activity, hydrolyzing purine nucleotide phosphoramidates with a single phosphate group such as adenosine 5'monophosphoramidate (AMP-NH2) to yield AMP and NH2. Hydrolyzes adenosine 5'monophosphomorpholidate (AMP-morpholidate) and guanosine 5'monophosphomorpholidate (GMP-morpholidate). Hydrolyzes lysyl-AMP (AMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) generated by lysine tRNA ligase, as well as Met-AMP, His-AMP and Asp-AMP, lysyl-GMP (GMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) and AMP-N-alanine methyl ester. Hydrolyzes 3-indolepropionic acyl-adenylate, tryptamine adenosine phosphoramidate monoester and other fluorogenic purine nucleoside tryptamine phosphoramidates in vitro. Can also convert adenosine 5'-O-phosphorothioate and guanosine 5'-O-phosphorothioate to the corresponding nucleoside 5'-O-phosphates with concomitant release of hydrogen sulfide. In addition, functions as scaffolding protein that modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex and by the complex formed with MITF and CTNNB1. Modulates p53/TP53 levels and p53/TP53-mediated apoptosis. Modulates proteasomal degradation of target proteins by the SCF (SKP2-CUL1-F-box protein) E3 ubiquitin-protein ligase complex. Also exhibits SUMO-specific isopeptidase activity, deconjugating SUMO1 from RGS17. Deconjugates SUMO1 from RANGAP1.
  • 基因功能参考文献：
    1. Thus, hHint1 appears to be structurally sensitive to irreversible inactivation by copper, which may be of neurotoxicological and pharmacological significance. PMID: 28739258
    2. Hint1 has been shown to be essential for the metabolic activation of nucleotide antiviral pronucleotides (i.e., proTides). These results provide mechanistic insights underpinning histidine nucleophilic catalysis in general and human Hint1 catalysis, in particular, thus aiding the design of future proTides and the elucidation of the natural function of the Hint family of enzymes. PMID: 28691797
    3. We provide a comprehensive overview of the structural and functional characteristics of the HINT1 protein that may guide further studies into the molecular aetiology and treatment strategies--{REVIEW} PMID: 28007994
    4. Underexpression of Hint1 is associated with invasion of hepatocellular carcinoma. PMID: 27623945
    5. first structure (at a 2.34A resolution) of a complex of human HINT1 with a non-hydrolyzable analog of an Ap4A dinucleotide PMID: 26905466
    6. Absence of HINT1 mutations in a UK and Spanish cohort of patients with inherited neuropathies PMID: 26194197
    7. In hepatocellular carcinoma, HINT1 is a regulator of IkappaBalpha through SCF(beta-TrCP) E3 ligase. PMID: 26520111
    8. HINT1 mutations seem to be one of the most frequent causes of inherited neuropathy and are probably the most frequent cause of HMN in Czech patients. We suggest all HMN/CMT2 patients be tested for the presence of the prevalent mutation, the p.R37P PMID: 25342199
    9. The teneurin-1 intracellular domain binds HINT1, thus switching on MITF-dependent transcription of GPNMB. PMID: 25648896
    10. Data suggest that histidine triad nucleotide binding protein 1 (HINT1) should be added to the list of genes to check for in distal hereditary motor neuropathies (dHMNs). PMID: 24105373
    11. Study gives suggestive evidence that HINT1 rs3852209 may be related to smoking status in Chinese men. PMID: 24447405
    12. BTF3, HINT1, NDRG1 and ODC1 protein over-expression in human prostate cancer tissue PMID: 24386364
    13. Histidine triad nucleotide-binding protein 1, bifunctional aminoacyl-tRNA synthetase, and clusterin precursor protein may serve as potential biomarkers distinguishing type 2 diabetes mellitus patients from healthy controls. PMID: 23769013
    14. hHint1 catalyzes the hydrolysis of phosphoramidate and acyl adenylate with high efficiency. PMID: 23614568
    15. loss of functional HINT1 protein results in a distinct phenotype of autosomal recessive axonal neuropathy with neuromyotonia. PMID: 22961002
    16. The results reveal the structural basis for the remarkable adenylate surveillance activity of Hint1, to potentially control Ap(4)A levels in the cell. PMID: 22329685
    17. structure of the human HINT1-adenosine 5'-monophosphate (AMP) complex at 1.38 A resolution obtained from a new monoclinic crystal form is reported PMID: 22869114
    18. The tumor suppressor activity of HINT1 gene in melanoma cells by promoting the formation of non-functional complexes with oncogenic transcription factors, like MITF and beta-catenin, known to be involved in melanoma development and progression. PMID: 22647378
    19. evaluation of potential inhibitors of human and Escherichia coli histidine triad nucleotide binding proteins PMID: 22104145
    20. This study demonistrated that HINT1 is found to be downregulated in the dorsolateral prefrontal cortex and upregulated in the thalamus. PMID: 21553311
    21. Results indicate a genetic association between HINT1 variants and ND, and indicate that nicotine-induced modulation of HINT1 level may be involved in mechanisms of excess smoking. PMID: 20514075
    22. Hint1 expression was lower in gastric cancer (GC) tissues. PMID: 21468541
    23. HINT1 is differentially expressed in schizophrenia brain. PMID: 21553311
    24. Hint1 exerts its major cellular function as gene transcription regulator, and thus, this function provides its potential role as a tumor suppressor protein. PMID: 20499681
    25. Reduction of nucleoside diphosphate kinase B, Rab GDP-dissociation inhibitor beta and histidine triad nucleotide-binding protein in fetal Down syndrome brain. PMID: 11771757
    26. the Hint1 pro-apoptotic activity is independent of the Hint1 enzymatic activity PMID: 16835243
    27. The major function of PKCI-1 is to modulate mu opioid receptor signaling pathway along with RGSZ1, rather than directly mediating the Galphaz RGSZ1 interaction. PMID: 17126529
    28. Human Hint1/Eoli HinT chimera does not exhibit a preference for phosphoramidates containing d- or l- tryptophan, while the E coli/human chimera adopts the human enzyme preference for the l configuration. PMID: 17939685
    29. Activation of protein kinase G Increases the expression of p21CIP1, p27KIP1, and histidine triad protein 1 through Sp1. PMID: 18593937
    30. variants at HINT1 may be associated with schizophrenia and the associations may be sex-specific. PMID: 18799291
    31. relationship between Hint1 methylation status and clinical features and other, previously measured biomarkers was also analyzed. p16 hypermethylation was statistically significantly associated with Hint1 methylation status PMID: 19081673
    32. decreased expression of the Hint1 gene through epigenetic silencing may play a role in enhancing the growth of a subset of human hepatoma by increasing the expression of genes controlled by the transcription factors beta-catenin, USF2, and NFkappaB. PMID: 19089909
    33. HINT1 up-regulates cellular levels of p27(KIP1) by two mechanisms: 1) it inhibits its ubiquitylation by targeting the SCF(SKP2) ubiquitin ligase complex, and 2) it inhibits the phosphorylation of p27(KIP1) by Src via inhibiting Src expression. PMID: 19112177
    34. Observational study of gene-disease association, gene-gene interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator) PMID: 17978999
    35. Hint is an adenosine 5'-monophosphoramidase. Hint does not bind zinc, hydrolyze diadenosine polyphosphates, not inhibit protein kinase C. In yeast, Hnt1 is a positive regulator of Kin28 (human Cdk7) and enzyme activity is required for this function. PMID: 11805111

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  • 相关疾病：
    Neuromyotonia and axonal neuropathy, autosomal recessive (NMAN)
  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    HINT family
  • 组织特异性：
    Widely expressed.
  • 数据库链接：

    HGNC: 4912

    OMIM: 137200

    KEGG: hsa:3094

    STRING: 9606.ENSP00000304229

    UniGene: Hs.483305