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  4. Recombinant Human Homeobox protein Nkx-3.1 (NKX3-1)

Recombinant Human Homeobox protein Nkx-3.1 (NKX3-1)

  • 中文名称：
    人NKX3-1重组蛋白
  • 货号：
    CSB-YP859523HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人NKX3-1重组蛋白
  • 货号：
    CSB-EP859523HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人NKX3-1重组蛋白
  • 货号：
    CSB-EP859523HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人NKX3-1重组蛋白
  • 货号：
    CSB-BP859523HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人NKX3-1重组蛋白
  • 货号：
    CSB-MP859523HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    NKX3-1
  • Uniprot No.：
  • 别名：
    BAPX 2; BAPX2; Homeobox protein NK-3 homolog A; Homeobox protein Nkx 3.1; Homeobox protein Nkx-3.1; Homeobox protein Nkx3.1; NK homeobox (Drosophila) family 3 A; NK homeobox family 3 A; NK homeobox, family 3, member A; NK3 homeobox 1; NK3 transcription factor homolog A; NK3 transcription factor related locus 1; NKX 3; Nkx 3.1; NKX 3A; NKX3 1; NKX3; Nkx3-1; NKX3.1; Nkx3.1, mouse, homolog of; NKX31_HUMAN; NKX3A
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-234
  • 氨基酸序列
    MLRVPEPRPG EAKAEGAAPP TPSKPLTSFL IQDILRDGAQ RQGGRTSSQR QRDPEPEPEP EPEGGRSRAG AQNDQLSTGP RAAPEEAETL AETEPERHLG SYLLDSENTS GALPRLPQTP KQPQKRSRAA FSHTQVIELE RKFSHQKYLS APERAHLAKN LKLTETQVKI WFQNRRYKTK RKQLSSELGD LEKHSSLPAL KEEAFSRASL VSVYNSYPYY PYLYCVGSWS PAFW
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcription factor, which binds preferentially the consensus sequence 5'-TAAGT[AG]-3' and can behave as a transcriptional repressor. Plays an important role in normal prostate development, regulating proliferation of glandular epithelium and in the formation of ducts in prostate. Acts as a tumor suppressor controlling prostate carcinogenesis, as shown by the ability to inhibit proliferation and invasion activities of PC-3 prostate cancer cells.
  • 基因功能参考文献：
    1. The tumour suppressors FOXP1 and NKX3.1, strongly implicated in PCa development, were identified as key transcription factors regulating TPbeta expression through Prm3 in both PCa cell lines. PMID: 28890397
    2. NKX3.1 has potential utility as a prostate specific marker in the differential diagnostic distinction between nephrogenic adenoma and prostatic adenocarcinoma. PMID: 28581204
    3. Prostate cancer patients with low NKX3.1 expression are optimal candidates for intervention with 5alpha-reductase inhibitors as an adjunct to active surveillance. PMID: 28385453
    4. Low NKX3.1 expression is associated with hepatocellular carcinoma. PMID: 28972178
    5. During prostate cancer initiation, Nkx3.1 expression is frequently lost in both humans and mouse models. Data, including data from studies using cells from transgenic mice, suggest that androgens activate Nkx3.1 transcription via androgen receptor binding to 11-kb region in both normal luminal cells and castration-resistant prostatic neoplasm cells via androgen response elements in Nkx3.1 3prime untranslated region. PMID: 28679531
    6. With deletion mutation analysis, plasmid construction, EMSA and oligonucleotide decoy technique, two Sp1-elements which located between +29 to +43 and -60 to -46 of NKX3.1 gene were identified and proven to be functional elements. PMID: 19263243
    7. The androgen-regulated homeodomain transcription factor NKX3.1 plays roles in early prostate development and functions as a prostate-specific tumor suppressor. PMID: 26841725
    8. the association between the down-regulation of PTEN and NKX3.1 genes contributed to prostate tumorigenesis. PMID: 26689514
    9. NKX3.1 and DYRK1B were shown to interact via the DYRK1B kinase domain. In vitro kinase assay showed that DYRK1B phosphorylated NKX3.1 at serine 185, a residue critical for NKX3.1 steady-state turnover. PMID: 25777618
    10. An NKX3.1 binding site polymorphism in the l-plastin promoter leads to differential gene expression in human prostate cancer PMID: 26148677
    11. Analysis of prostate cancer tissues showed that the presence of a TMPRSS2-ERG rearrangement was highly correlated with lower levels of NKX3.1 expression consistent with the role of NKX3.1 as a suppressor of the pathogenic gene rearrangement. PMID: 25977336
    12. NKX3.1 expression mediate beta-catenin and E-cadherin association and cell migration in prostate cells. PMID: 25360740
    13. Our results indicate that variation in NKX3.1 expression combined with selenium or vitamin E treatment modifies the risk of prostate cancer. PMID: 24894197
    14. Id4 regulates NKX3.1, Sox9 and PTEN. PMID: 23786676
    15. NKX3.1 labelling is limited to estrogen/progesterone/androgen receptor-positive breast carcinomas. PMID: 24996432
    16. These observations imply that the frequently noted loss-of-function of NKX3.1 cooperates with the activation of TMPRSS2-ERG fusions in prostate tumorigenesis. PMID: 24418414
    17. NKX3.1 may enhance DNA integrity in prostate stem cells and may help to explain how cells differ in their sensitivity to DNA damage. PMID: 23890999
    18. Androgens and NKX3.1 expression regulate the progression of the cell cycle and concurrently activate the DNA damage response. PMID: 24434284
    19. Loci at 8q24 and 8q21 could influence susceptibility to prostate cancer in the northern Chinese population PMID: 24779299
    20. multiple NKX3.1 binding sites were found in the RAMP1 locus in human prostate cancer cells and in the normal mouse prostate. PMID: 23867798
    21. NKX3-1 is a tumor suppressor gene through direct regulation of the cell cycle and growth/survival pathways. PMID: 23836514
    22. Pim-1-mediated NKX3.1 stabilization may be important in maintaining normal cellular homeostasis in normal prostate epithelial cells, and may maintain basal NKX3.1 protein levels in prostate cancer cells. PMID: 23129228
    23. Transcriptional activation of prostate specific homeobox gene NKX3-1 in subsets of T-cell lymphoblastic leukemia (T-ALL). PMID: 22848398
    24. PDLIM5 (rs17021918,T), SLC22A3 (rs9364554,C) and NKX3-1 (rs1512268,A) SNPs might not be associated with prostate cancer in Chinese men. PMID: 22741436
    25. Proline-mediated proteasomal degradation of the prostate-specific tumor suppressor NKX3.1. PMID: 22910912
    26. NKX3.1 inhibits IGF-1R expression and its signalling pathway in human prostatic carcinoma PC3 cells. PMID: 22179513
    27. loss of NKX3-1 may be a potential biomarker for occult lymph node metastasis and poor prognosis in oral squamous cell carcinoma. PMID: 22344708
    28. NKX3-1 and androgen receptor directly regulate each other in a feed-forward regulatory loop. PMID: 22083957
    29. androgen regulated NKX3.1 maintains an active DNA damage response at the intra S progression and contributes to the chemotherapeutic resistance of prostate cancer cells to DNA damaging compounds. PMID: 21945437
    30. Copy number gain of the MIR151 gene and/or loss of the NKX3-1 gene in the primary tumor may indicate the presence of metastatic prostate cancer. PMID: 21456068
    31. results suggest that functional variant rs11781886 in 5'-UTR of NKX3.1 can affect its transcription by altering the binding affinity of a transcriptional factor Sp1, and might result in PC susceptibility by lowering expression of NKX3.1 in the prostate PMID: 20716579
    32. ETS1 upregulation of NKX3.1 expression in LNCaP cells is mediated in part via its interaction with an EBS located in the NKX3.1 5' proximal promoter. PMID: 20842667
    33. in human TAL1-expressing T-ALL cell lines, TAL1 directly activates NKX3.1 PMID: 20855495
    34. Nkx3.1 has a role in bacterial prostatitis and its progression to inflammation and neoplasia PMID: 20363913
    35. The prostate-specific tumor suppressor gene Nkx3.1 was controlled by ERG and ESE3 both directly and through induction of EZH2. PMID: 20479932
    36. The addition of NKX3.1 protein staining to a panel of markers, if applied in the appropriate clinicopathologic context, may add diagnostic value in the diagnosis of metastatic lesions of unknown primary origin. PMID: 20588175
    37. NKX3.1 might exert its function by regulating the expression of relative genes in prostate development and carcinogenesis. PMID: 19462257
    38. NKX3.1 activates cellular response to DNA damage PMID: 20395202
    39. Androgen regulation of the prostatic tumour suppressor NKX3.1 is mediated by its 3' untranslated region PMID: 19886863
    40. NKX-3.1 interacts with prostate-derived Ets factor and regulates the activity of the PSA promoter. PMID: 11809674
    41. smooth muscle gamma actin gene activity in prostate epithelia is due, in part, to the androgen-dependent expression of Nkx 3.1 PMID: 12450213
    42. Expression of NKX3.1 is highly restricted and is found primarily in benign and malignant prostatic epithelial cells and also in normal testis and lobular carcinoma of the breast. PMID: 12661036
    43. NKX3.1 is essential for normal testis function and that its loss of expression is highly associated with the invasive phenotype of testicular germ cell tumors. PMID: 14633588
    44. Nkx3.1 homeobox gene has roles in prostate organogenesis and carcinogenesis (review) PMID: 14648854
    45. NKX3.1 does not function as a typical tumor suppressor protein in prostate cancer but it may still have important regulatory roles during prostate cancer progression PMID: 15311057
    46. identified novel protein-protein interaction domains within NKX-3.1 and PDEF that operate in concert with their respective DNA binding domains to mediate functional interactions between these growth regulatory transcription factors PMID: 15523673
    47. Down-regulation of NKX3.1 expression is not caused by promoter hypermethylation in testicular germ cell tumors and prostate carcinomas. PMID: 15691383
    48. Even in the presence of allelic loss, NKX3.1 expression is reduced over a wide range in prostate cancer. PMID: 15734999
    49. We found that the 20 bp inhibitory decoy could enhance NKX3.1 promoter activity, and RT-PCR and Western blot analysis revealed that NKX3.1 expression was up-regulated effectively by the transfection with the 20 bp inhibitory decoy. PMID: 15880262
    50. Nkx3.1 negatively regulates Sp-mediated transcription via the tethering of histone deacetylases and/or by inhibiting the association of Sp proteins with co-activators. PMID: 16201967

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    NK-3 homeobox family
  • 组织特异性：
    Highly expressed in the prostate and, at a lower level, in the testis.
  • 数据库链接：

    HGNC: 7838

    OMIM: 602041

    KEGG: hsa:4824

    STRING: 9606.ENSP00000370253

    UniGene: Hs.55999