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  4. Recombinant Human Homeobox protein engrailed-2 (EN2)

Recombinant Human Homeobox protein engrailed-2 (EN2)

  • 中文名称：
    人EN2重组蛋白
  • 货号：
    CSB-YP007660HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人EN2重组蛋白
  • 货号：
    CSB-EP007660HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人EN2重组蛋白
  • 货号：
    CSB-EP007660HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人EN2重组蛋白
  • 货号：
    CSB-BP007660HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人EN2重组蛋白
  • 货号：
    CSB-MP007660HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    EN2
  • Uniprot No.：
  • 别名：
    EN2; Homeobox protein engrailed-2; Homeobox protein en-2; Hu-En-2
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-333
  • 氨基酸序列
    MEENDPKPGE AAAAVEGQRQ PESSPGGGSG GGGGSSPGEA DTGRRRALML PAVLQAPGNH QHPHRITNFF IDNILRPEFG RRKDAGTCCA GAGGGRGGGA GGEGGASGAE GGGGAGGSEQ LLGSGSREPR QNPPCAPGAG GPLPAAGSDS PGDGEGGSKT LSLHGGAKKG GDPGGPLDGS LKARGLGGGD LSVSSDSDSS QAGANLGAQP MLWPAWVYCT RYSDRPSSGP RSRKPKKKNP NKEDKRPRTA FTAEQLQRLK AEFQTNRYLT EQRRQSLAQE LSLNESQIKI WFQNKRAKIK KATGNKNTLA VHLMAQGLYN HSTTAKEGKS DSE
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 基因功能参考文献：
    1. The EN2 transcription factor is a novel ovarian cancer biomarker. It demonstrates prognostic value, correlating with worse Overall Survival and Progression-free Survival. PMID: 30285763
    2. we confirmed that engrailed-2 is the target of hsa-miR-27b in human cytomegalovirus-infected glioma cells PMID: 28343438
    3. The critical role of miR212 in inhibiting prostate tumor growth was verified in xenograft models of prostate cancer. These findings highlighted the role of miR212 in PCa progression. More importantly, we speculate that EN-2 is a direct target gene of miR212. PMID: 28713997
    4. We identified a novel heterozygous missense variant (c.425C>G, p.Pro142Arg) in the Engrailed 2 (EN2) gene in two patients. The G variant has never been reported in public databases and was absent in 100 Thai patients with ASD and 435 Thai controls. A case-control study showed that the G allele of c.425C>G was significantly associated with ASD PMID: 27755371
    5. EN2 is negatively regulated by miR-605, and down-regulation of miR-605 promotes the proliferation and invasion of prostate cancer (PCa) cells by up-regulating EN2, which leads to PCa development and progression PMID: 28943214
    6. Our data suggest that the functional interaction of miR-33a and EN-2 is involved in tumorigenesis of prostate cancer PMID: 27921232
    7. the results demonstrated that EN2 might play an anti-oncogenic role in oncogenesis and development of CCRCC, thereby maintaining the normal growth of human renal tubular epithelial cells. PMID: 26948025
    8. EN2 may be a candidate oncogene in bladder cancer by activating the PI3K/Akt pathway and inhibiting PTEN. PMID: 25812440
    9. Engrailed-2 (EN-2) is a developmentally regulated homeobox gene that is essential for Purkinje cell maturation and normal cerebellar development and patterning. PMID: 25290267
    10. The mean EN2 levels in prostate cancer patients were 3.76-fold higher than those in non-prostate patients after prostate massage. PMID: 25003607
    11. The exact role of EN2 expression in renal carcinoma carcinogenesis requires further investigation. PMID: 24532139
    12. En2 identified as a new regulator of T-cell differentiation whose disruption induces a malignant T-cell fate in chronic myelogenous leukemia progression. PMID: 24675889
    13. Two patients with underdeveloped arcuate fasciculus had novel, nonsynonymous variants in MID1 and EN2 genes regulating axon guidance pathway. PMID: 24321989
    14. EN2 levels are increased in affected individuals with an A-C/G-T genotype, supporting EN2 as an autism spectrum disorder susceptibility gene. PMID: 24520327
    15. urinary EN2 has the potential to be a more sensitive and specific protein biomarker for non-muscle-invasive bladder cancer than currently available tests. PMID: 23434148
    16. Urinary EN2 may be useful as a non-invasive early biomarker for prostate cancer detection in genetically high-risk individuals PMID: 23792811
    17. Normal EN-2 downregulation may not occur in some individuals with autism, such that the postnatal persistence of EN-2 overexpression may contribute to autism cerebellar abnormalities. PMID: 23423141
    18. The WNT2 gene and EN2 gene may act in concert to influence the language development in autism. PMID: 22522212
    19. findings show EN2 was expressed and secreted by prostate cancer (PC) cell lines and PC tissue but not by normal prostate tissue or stroma; presence of EN2 in urine was highly predictive of PC PMID: 21364037
    20. This study demonistrated that in Han chinese the association between the EN2 genetic polymorphism and autism. PMID: 20523082
    21. Positive genetic correlation of EN2 with autism in the Indian population. PMID: 20050924
    22. In human, failure to form a mesencephalon and metencephalon, with cerebellar hypoplasia, results drom a mutation or deletion in EN2(Engrailed-2) gene. PMID: 11815869
    23. EN2 exon 1 variant transmission disequilibrium test shows no association with autistic disorder nor association between language and stereotypic behaviour quantitative traits. PMID: 12525552
    24. An association between a cerebellar patterning gene and autism spectrum disorder suggests a role for EN2 as a susceptibility locus and supports a neurodevelopmental defect hypothesis in the etiology of autism. PMID: 15024396
    25. RNA interference studies suggest that EN2 expression is required for the maintenance of the transformed phenotype of a human breast tumor cell line PMID: 16007149
    26. Loss and gain of function experiments firmly establish that En1/2 is a true survival factor for dopaminergic neurons in vivo. PMID: 17267560
    27. Heterogeneous association between engrailed-2 and autism in the CPEA network PMID: 17948868
    28. a preferential transmission of the rs3824068 A-allele to AUTISM affected offspring PMID: 17948901
    29. Engrailed 2 gene is associated with autistic disorder in Chinese children. PMID: 18424904
    30. The majority of regulated genes were down-regulated in engrailed-2 cerebella from transgenic mice, consistent with the known repressive function of Engrailed-2. PMID: 18562208
    31. variations of the engrailed-2 gene are implicated in the development of young-onset Parkinson's disease PMID: 19270442
    32. The data of this study indicated that the A-C haplotype is functional and, together with the association and LD mapping results, supports EN2 as a likely autism susceptibility gene and the A-C haplotype as a possible risk allele. PMID: 19615670

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  • 相关疾病：
    Genetic variations in EN2 may be associated with susceptibility to autism.
  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Engrailed homeobox family
  • 数据库链接：

    HGNC: 3343

    OMIM: 131310

    KEGG: hsa:2020

    STRING: 9606.ENSP00000297375

    UniGene: Hs.134989