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  4. Recombinant Human Insulin gene enhancer protein ISL-1 (ISL1)

Recombinant Human Insulin gene enhancer protein ISL-1 (ISL1)

  • 货号：
    CSB-YP011846HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP011846HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP011846HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP011846HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP011846HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Insulin gene enhancer protein ISL 1; Insulin gene enhancer protein ISL-1; Insulin related protein; ISL 1; ISL LIM homeobox 1; ISL1; ISL1 transcription factor LIM homeodomain; ISL1 transcription factor, LIM/homeodomain (islet 1); ISL1 transcription factor, LIM/homeodomain; ISL1_HUMAN; Islet-1; Islet1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-349
  • 氨基酸序列
    MGDMGDPPKK KRLISLCVGC GNQIHDQYIL RVSPDLEWHA ACLKCAECNQ YLDESCTCFV RDGKTYCKRD YIRLYGIKCA KCSIGFSKND FVMRARSKVY HIECFRCVAC SRQLIPGDEF ALREDGLFCR ADHDVVERAS LGAGDPLSPL HPARPLQMAA EPISARQPAL RPHVHKQPEK TTRVRTVLNE KQLHTLRTCY AANPRPDALM KEQLVEMTGL SPRVIRVWFQ NKRCKDKKRS IMMKQLQQQQ PNDKTNIQGM TGTPMVAASP ERHDGGLQAN PVEVQSYQPP WKVLSDFALQ SDIDQPAFQQ LVNFSEGGPG SNSTGSEVAS MSSQLPDTPN SMVASPIEA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    DNA-binding transcriptional activator. Recognizes and binds to the consensus octamer binding site 5'-ATAATTAA-3' in promoter of target genes. Plays a fundamental role in the gene regulatory network essential for retinal ganglion cell (RGC) differentiation. Cooperates with the transcription factor POU4F2 to achieve maximal levels of expression of RGC target genes and RGC fate specification in the developing retina. Involved in the specification of motor neurons in cooperation with LHX3 and LDB1. Binds to insulin gene enhancer sequences. Essential for heart development. Marker of one progenitor cell population that give rise to the outflow tract, right ventricle, a subset of left ventricular cells, and a large number of atrial cells as well, its function is required for these progenitors to contribute to the heart. Controls the expression of FGF and BMP growth factors in this cell population and is required for proliferation and survival of cells within pharyngeal foregut endoderm and adjacent splanchnic mesoderm as well as for migration of cardiac progenitors into the heart.
  • 基因功能参考文献：
    1. ISL1 is a major susceptibility gene for bladder exstrophy and a regulator of urinary tract development. PMID: 28176844
    2. Mechanistic analyses integrate this unrecognized anti-atrial function of ISL1 with known and newly identified atrial inducers. In this revised view, ISL1 is antagonized by retinoic acid signaling via a novel player, MEIS2. PMID: 29337667
    3. Data show that Islet-1 (ISL1) activated the expression of cyclin B1 (CCNB1), cyclin B2 (CCNB2) and c-myc (c-MYC) genes by binding to the conserved binding sites on their promoters or enhancers. PMID: 27183908
    4. results identify Isl1 as a crucial transcription factor that plays essential roles in the gene regulatory program directing development of multiple arcuate neuronal subpopulations. PMID: 27578785
    5. ISL-1 is widely expressed in Olfactory neuroblastoma tumors with neuroendocrine differentiation and therefore of limited value in their differential diagnosis PMID: 27543867
    6. Data indicate that cells cultured on cardiac muscle laminin (LN)-based substrata in combination with stimulation of the canonical Wnt/beta-catenin pathway showed increased gene expression of ISL1, OCT4, KDR and NKX2.5. PMID: 27052314
    7. In bladder exstrophy there is a clear correlation with the a mutation of the chromosome 5 ISL1 (5q11.1) gene. PMID: 26077299
    8. SSBP3 Interacts With Islet-1 and Ldb1 to Impact Pancreatic beta-Cell Target Genes PMID: 26495868
    9. Concurrent ISL1/HOXA9 methylation in HG-NMIBC reliably predicted tumour recurrence and progression within one year (Positive Predictive Value 91.7%), and was associated with disease-specific mortality PMID: 26332997
    10. Isl1 overexpression in embryonic stem cells results in normal electrophysiologically functioning cells. PMID: 25524439
    11. The present study identified the first genome-wide significant locus for classic bladder exstrophy at chromosomal region 5q11.1, and provides strong evidence for the hypothesis that ISL1 is the responsible candidate gene in this region. PMID: 25763902
    12. Suggest that ISL-1 may be a useful prognostic biomarker and may represent a novel therapeutic target for gastric adenocarcinoma. PMID: 26142548
    13. ISL-1 is tightly linked to non-Hodgkin lymphoma proliferation and development by promoting c-Myc transcription, and its aberrant expression was regulated by p-STAT3/p-c-Jun/ISL-1 complex activation. PMID: 25070240
    14. Overexpression of ISL1 in human mesnchymal stem cells promotes angiogenesis in vitro and in vivo through increasing secretion of MCP3 and other paracrine factors. PMID: 24578274
    15. ISL1 is commonly expressed in rhabdomyosarcoma, particularly the alveolar subtype PMID: 24751901
    16. Results suggest that rs1017 ISL1 contributed to the risk of Congenital heart disease (CHD) in Chinese Han people, and the gene may be involved in the formation and development of the heart. PMID: 24634231
    17. This study demonstrates that the Isl1-Gata3 transcription regulatory axis is essential for normal pyloric development. PMID: 24674670
    18. these results demonstrate a positive feedback regulation of ISL-1 in diffuse large B cell lymphoma but not in pancreatic beta-cells, which might result in the functional diversities of ISL-1 in different physiological and pathological processes. PMID: 24845569
    19. We have demonstrated that Islet 1 and PAX8 are not entirely specific for neuroendocrine tumors of pancreatic origin, as they are expressed in a majority of rectal neuroendocrine tumors. PMID: 24037217
    20. Overexpression of VEGF-A promotes not only the endothelial specification but also engraftment, proliferation and survival (reduced apoptosis) of the human Isl1+ progenitors in vivo. PMID: 24018375
    21. Haplotype TT of ISL1 may increase the risk of congenital heart disease (CHD)in Tianjin Han children. PMID: 24131831
    22. findings modify the role of ISL1 as a marker for pancreatic neuroendocrine neoplasms and suggest that ISL1 has a broader involvement in differentiation and growth of neuroendocrine neoplasms than has so far been assumed. PMID: 23503646
    23. The methylation status of Homeobox A9 (HOXA9), ISL LIM homeobox 1 (ISL1) and Aldehyde dehydrogenase 1 family, member A3 (ALDH1A3) was significantly associated with decreased gene expression levels PMID: 23436614
    24. The findings suggest that ISL1 genetic polymorphisms are associated with occurrence of VSD, thus they may be useful as molecular markers for prediction of VSD. PMID: 23572340
    25. ISL1 common variant rs1017 is not associated with increased genetic risk of CHD in the white population. PMID: 23229290
    26. This study describes a new gain-of-function p.Asn252Ser variant in the human ISL1 gene, which could potentially lead to greater activation of downstream targets involved in cardiac development, dilation, and hypertrophy. PMID: 23152444
    27. Our findings confirm that Isl1 is a sensitive marker of pancreatic origin in cases of metastatic neuroendocrine neoplasms . PMID: 23348208
    28. This is the first study which indicates that ISL1 common variant rs1017 may not play a role in sporadic CHD susceptibility in the Chinese population. PMID: 22480195
    29. Both Islet 1 and PAX8 are reliable immunohistochemical markers for identifying metastatic neuroendocrine tumors of pancreatic origin. PMID: 22388755
    30. The relationship between human ISL1 and FGF10 within the embryonic time window during which the linear heart tube remodels into four chambers, was examined. PMID: 22303449
    31. This study establishes ISL1 as a novel transcriptional regulator of LHX3 and describes a potential mechanism for regulation by PITX1. PMID: 22194342
    32. Data indicate that, despite the relevant role of ISL1 in thyroid and heart morphogenesis, mutations in its coding region are not associated with TD in our group of patients. PMID: 21060249
    33. Two different ISL1 haplotypes contribute to risk of congenital heart disease in white and black/African American populations. PMID: 20520780
    34. early first trimester human embryonic heart expresses clusters of Isl1(+)cells, some of which differentiate into cardiomyocytes. PMID: 19896915
    35. Seven single nucleotide polymorphisms (SNPs) and one microsatellite in noncoding regions of ISL1 GENE. Four haplotypes formed by six of these SNPs and one microsatellite were associated with type 1 diabetes in Swedish families. PMID: 15161765
    36. we demonstrated that ISL1 and BETA2 could activate insulin gene transcription synergistically. PMID: 16321656
    37. cultured pancreatic islets contain nestin and Isl-1 positive mesenchymal stem cells with multipotential developmental capacity PMID: 16713999
    38. Isl1 could enhance the HNF4alpha-mediated activation of transcription of the HNF1alpha, PPARalpha and insulin I promoters PMID: 17022998
    39. Sudy illustrated that GPA2 is positively regulated by isl-1, suggesting that this protein associates with endocrine systems including the pituitary and pancreas. PMID: 17363077
    40. beta-catenin signaling regulates proliferation and survival of cardiac progenitors PMID: 17519333
    41. An important developmental and regulatory gene ISL1 is uniquely expressed in visceral fat, probably in the preadipocyte. ISL1 may be regulated by adiposity. PMID: 18239644
    42. Although the LIM interaction domain of Ldb1 (Ldb1(LID)) and Isl1(LBD) share low levels of sequence homology, X-ray and NMR structures reveal that they bind Lhx3 in an identical manner, that is, Isl1(LBD) mimics Ldb1(LID). PMID: 18583962
    43. purified ISL1(+) primordial progenitors are capable of self-renewal and expansion before differentiation into the three major cell types in the heart PMID: 19571884
    44. These results suggest that ISL1 is a transcriptional activator for insulin gene expression, and the interactions of ISL1 with BETA2 are required for the transcriptional activity of the insulin gene. PMID: 19619559

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  • 亚细胞定位：
    Nucleus.
  • 组织特异性：
    Expressed in subsets of neurons of the adrenal medulla and dorsal root ganglion, inner nuclear and ganglion cell layers in the retina, the pineal and some regions of the brain.
  • 数据库链接：

    HGNC: 6132

    OMIM: 600366

    KEGG: hsa:3670

    STRING: 9606.ENSP00000230658

    UniGene: Hs.505