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  4. Recombinant Human Kallikrein-1 (KLK1)

Recombinant Human Kallikrein-1 (KLK1)

  • 货号：
    CSB-YP012446HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP012446HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP012446HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP012446HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Glandular kallikrein 1; hK 1; hK1; Kallikrein serine protease 1; Kallikrein-1; Kidney/pancreas/salivary gland kallikrein; Klk 6; KLK1; KLK1_HUMAN; Klk6; KLKR; Tissue kallikrein
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    25-262
  • 氨基酸序列
    IVGGWE CEQHSQPWQA ALYHFSTFQC GGILVHRQWV LTAAHCISDN YQLWLGRHNL FDDENTAQFV HVSESFPHPG FNMSLLENHT RQADEDYSHD LMLLRLTEPA DTITDAVKVV ELPTEEPEVG STCLASGWGS IEPENFSFPD DLQCVDLKIL PNDECKKAHV QKVTDFMLCV GHLEGGKDTC VGDSGGPLMC DGVLQGVTSW GYVPCGTPNK PSVAVRVLSY VKWIEDTIAE NS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.
  • 基因功能参考文献：
    1. Our findings suggest that the A2233C polymorphism of KLK1 may be a marker of evaluation of hypertensive subjects' responses to angiotensin I converting enzyme inhibitors benazepril. PMID: 28621557
    2. Recognition of anti-tumor necrosis factor-alpha (TNF-alpha) or Kallikrein Inhibitor may lead to therapeutics to enhance existing treatments for patients who do not respond to anti-vascular endothelial growth factor (VEGF) therapies. PMID: 27618014
    3. increasing the serum levels of AngII increased the risk of acute myocardial infarction (AMI); the risk of AMI increased when the serum levels of AngII and KLK1 simultaneously increased; individuals with the combined genotypes of ACE DD and KLK1 GG showed increased risk of AMI compared with those with the combined genotypes of ACE II and KLK1 AA PMID: 27329205
    4. The rs5516 G allele of KLK1 was significantly associated with aortic aneurysm PMID: 27858843
    5. KLK1 rs5516 SNP is not related to the incidence of Alzheimer's disease in a Hunan Han Chinese population. PMID: 26884824
    6. TK promoted cell survival and beta-catenin degradation in serum-starved SH-SY5Y cells via increasing autophagy. PMID: 26677174
    7. Our findings suggest that higher levels of TK in plasma are associated with the presence of CAD and are a predictor of mild coronary arteriosclerosis. PMID: 24626253
    8. The kallikrein system in retinal damage/protection PMID: 25448306
    9. Preclinical characterization of recombinant human tissue kallikrein-1 as a novel treatment for type 2 diabetes mellitus. PMID: 25100328
    10. Up-regulation of KLK1 in tubular epithelial cells may mediate pro-inflammatory pathway and PAR activation during diabetic nephropathy. PMID: 24586431
    11. These data do not support a role for the Tissue kallikrein-kinin system, protective or deleterious, in the development of insulin resistance and diabetes. PMID: 24599937
    12. Tissue kallikrein facilitated the activation of EGFR, ERK1/2 and p38 cascade. Not p38 but ERK1/2 phosphorylation was severely compromised in cells depleted of EGFR. Impairment of signaling of ERK1/2 seemed not to be restricted to EGFR phosphorylation. PMID: 24530396
    13. allele H is a common polymorphism in Japanese and may contribute to decreased reabsorptions of calcium and sodium in the kidney PMID: 24005896
    14. Data suggest factor XII binding/autoactivation are increased on surface of hantavirus-infected vascular endothelium; thus, activation of kallikrein-kinin system during hantavirus infection could have profound implications on capillary permeability. PMID: 23874198
    15. Tissue kallikrein-modified mesenchymal stem cells provide enhanced protection against ischemic cardiac injury after myocardial infarction. PMID: 23697984
    16. KLK1 promoter polymorphisms are associated with development of AKI and adverse outcomes. Further studies are needed to validate these findings. PMID: 23635481
    17. Polymorphism of the KLK1 A1789G gene is associated with coronary artery stenosis. PMID: 23765970
    18. Unexpectedly, elevated KLK1 expression and excretion is found in patients with established or incipient acute kidney injury. PMID: 21679467
    19. Suggest that a genetic polymorphism in KLK1 may contribute to the risk of developing later stage abdominal aortic aneurysm. PMID: 21571276
    20. Neither rs5515 nor rs3212855 SNP is associated with cerebral hemorrhage. PMID: 21200088
    21. Data suggest that lower plasma tissue kallikrein levels are independently associated with first-ever stroke and are an independent predictor of recurrence after an initial stroke. PMID: 21823154
    22. rs5516 in the KLK1 gene may be involved in the development of essential hypertension. PMID: 20613781
    23. lung epithelial cells support the assembly and activation of the plasma kallikrein-kinin system by a mechanism dependent on HSP90, and could contribute to KKS-mediated inflammation in lung disease. PMID: 20536386
    24. Increased expression of hK1 by astrocytes co-localized with GFAP was found, contrasting with kinin B1 and B2 receptors, which were co-localized with NeuN in the sclerotic hippocampus PMID: 21211543
    25. Tissue kallikrein is essential for invasive capacity of circulating proangiogenic cells. PMID: 21164105
    26. The results indicate differential signaling pathways mediated by TK in promoting prostate cancer cell migration and invasion via PAR(1) activation, and proliferation via kinin B2 receptor stimulation. PMID: 20482314
    27. Results suggested that the rs5517 polymorphism was associated with cerebral hemorrhage, while the rs5516 polymorphism was not in Changsha Han Chinese. PMID: 20533273
    28. KLK1 gene polymorphisms are not associated with lupus nephritis in a Chinese Han population. PMID: 20516044
    29. Tissue kallikrein levels are increased in type 2 diabetes, and findings do not support a role for the kallikrein-kinin system in mediating the effects of statin therapy on endothelial function. PMID: 20225398
    30. elevated plasma levels in patients with hereditary angioedema PMID: 20143645
    31. we propose a model to illustrate how the two enhancers may work to regulate the transcription of PSA and hK2. PMID: 9857240
    32. Tissue kallikrein KLK1 is expressed de novo in endothelial cells and mediates relaxation of human umbilical veins. PMID: 11727832
    33. Association of the tissue kallikrein gene promoter with ESRD and hypertension. PMID: 11849458
    34. Loss-of-function polymorphism of the human kallikrein gene with reduced urinary kallikrein activity. PMID: 11912256
    35. Kinetic peculiarities of human tissue kallikrein PMID: 11913965
    36. endothelial cells synthesize and release an active form of tissue kallikrein - kinin generation on the surface may play an important role in maintenance of circulation homeostasis PMID: 12581867
    37. Diminution of kallikrein biosynthesis in African Americans seems to involve mechanisms at or distal to the aldosterone receptor, and perhaps at the level of the kallikrein gene itself. PMID: 12670744
    38. essentially unsusceptible to processing by human urinary kallikrein (tissue-type) PMID: 12887060
    39. that in the airways, monocytes, neutrophils, and alveolar macrophages may contribute to increased TK activity PMID: 14660481
    40. Sustained hyaluronan depolymerization is expected to cause tissue kallikrein activation, EGF release, and EGFR signaling. PMID: 14988406
    41. The K allele of KLK1 promoter and TT genotype of TGF-beta1 may be a genetic KLK1 -130 GN and -128 G-C, and the susceptibility factor contributing to progressive renal deterioration in Taiwanese primary vesicoureteric reflux children. PMID: 15086490
    42. Transduced human tissue kallikrein activated murine Akt-B through Ser-473 phosphorylation providing new information on the pathway involved in hTK-induced neoangiogenesis. PMID: 15364809
    43. transgenic rats expressing hKLK1 have an impaired renal response to acute volume expansion PMID: 15544850
    44. kallikrein/kinin protects against cardiomyocyte apoptosis in vivo and in vitro via Akt-Bad.14-3-3 and Akt-GSK-3beta-caspase-3 signaling pathways PMID: 15611141
    45. analysis of peptide inhibitor/substrate binding to human apo kallikrein 1 PMID: 15651049
    46. Induction of KLK1 in carotid arteriosclerosis does not lead to kallikrein-kinins pathway activation. PMID: 15662224
    47. Data describe the vascular, hormonal, and renal phenotypes of carriers of the loss-of-function polymorphism of the human tissue kallikrein gene. PMID: 15765151
    48. Gene delivery protects against rat diabetic cardiomyopathy by improving cardiac function and promoting glucose utilization and lipid metabo PMID: 15855348
    49. There are polymorphisms in regulatory region of human tissue kallikrein gene in Chinese Han people. Differences in both allele and genotype frequencies show association of hypertension with polymorphisms. PMID: 15905889
    50. the the kallikrein-kinin system has roles in intramyocardial inflammation, endothelial dysfunction and oxidative stress in diabetic cardiomyopathy PMID: 16129698

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  • 蛋白家族：
    Peptidase S1 family, Kallikrein subfamily
  • 组织特异性：
    Isoform 2 is expressed in pancreas, salivary glands, kidney, colon, prostate gland, testis, spleen and the colon adenocarcinoma cell line T84.
  • 数据库链接：

    HGNC: 6357

    OMIM: 147910

    KEGG: hsa:3816

    STRING: 9606.ENSP00000301420

    UniGene: Hs.123107