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  4. Recombinant Human Kallikrein-6 (KLK6)

Recombinant Human Kallikrein-6 (KLK6)

  • 中文名称：
    人KLK6重组蛋白
  • 货号：
    CSB-YP842165HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人KLK6重组蛋白
  • 货号：
    CSB-EP842165HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人KLK6重组蛋白
  • 货号：
    CSB-EP842165HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人KLK6重组蛋白
  • 货号：
    CSB-BP842165HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人KLK6重组蛋白
  • 货号：
    CSB-MP842165HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    KLK6
  • Uniprot No.：
  • 别名：
    Bssp; hK 6; hK6; Kallikrein 6 precursor; Kallikrein related peptidase 6; Kallikrein-6; Kallikrein6; KLK 6; Klk 7; KLK 9; Klk29; Klk6; KLK6_HUMAN; Klk7; KLK9; MGC9355; mGK 1; mGK1; MSP; Neurosin; Protease M; Protease serine 18; Protease serine 9; PRSS 18; PRSS 9; PRSS18; PRSS9; Serine protease 18; Serine protease 9; SP 59; SP59; Tissue kallikrein; TK; Zyme
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    22-244
  • 氨基酸序列
    LVHGGPCDK TSHPYQAALY TSGHLLCGGV LIHPLWVLTA AHCKKPNLQV FLGKHNLRQR ESSQEQSSVV RAVIHPDYDA ASHDQDIMLL RLARPAKLSE LIQPLPLERD CSANTTSCHI LGWGKTADGD FPDTIQCAYI HLVSREECEH AYPGQITQNM LCAGDEKYGK DSCQGDSGGP LVCGDHLRGL VSWGNIPCGS KEKPGVYTNV CRYTNWIQKT IQAK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Serine protease which exhibits a preference for Arg over Lys in the substrate P1 position and for Ser or Pro in the P2 position. Shows activity against amyloid precursor protein, myelin basic protein, gelatin, casein and extracellular matrix proteins such as fibronectin, laminin, vitronectin and collagen. Degrades alpha-synuclein and prevents its polymerization, indicating that it may be involved in the pathogenesis of Parkinson disease and other synucleinopathies. May be involved in regulation of axon outgrowth following spinal cord injury. Tumor cells treated with a neutralizing KLK6 antibody migrate less than control cells, suggesting a role in invasion and metastasis.
  • 基因功能参考文献：
    1. High KLK6 expression is associated with metastasis of colon cancer. PMID: 30015976
    2. Results showed a significant downregulation of KLK6 mRNA expression in breast cancer (BC) tissue sections compared to the non-cancerous component. The expression of KLK6 is positively associated with tumor grade and is overexpressed in aggressive phenotypes such as TNBC. PMID: 29435805
    3. KLK6-PAR2 expression is down-regulated and PAR1 is up-regulated when DeltaNp63beta expression is decreased, leading to EMT with enhancing migration and invasion through ERK signal reduction at the invasive front PMID: 29224812
    4. KLK6 may be the key protease involved in the proteolytic modification of PFK-M. PMID: 29563071
    5. Our data demonstrate for the first time increased levels of KLK6 in mucosal melanomas of the head and neck PMID: 27844409
    6. Induction of autophagic processes through KLK6 expression may increase acquisition of resistance of gastric cancer cells to auranofin-induced autophagy. PMID: 27863404
    7. Our data provide experimental evidence that KLK6 controls metastasis formation in colon cancer via specific downstream network of miRNA-mRNA effectors. PMID: 28431272
    8. Data show that kallikrein 6 (KLK6) expression is induced in mouse and human skin during chronic glucocorticoid treatment. PMID: 27283773
    9. FST and KLK6 may have significance in breast cancer detection PMID: 27168011
    10. KLK6 overexpression and likely its proteolytic activity is associated with alterations in downstream miRNAs and their targets, and these differ with the molecular subtypes of breast cancer. PMID: 27093921
    11. Molecular models of peptides were built into the KLK6 structure and the marked preference of the cut between the two R of the examined peptides was related to the extended conformation of the substrates PMID: 28254587
    12. Our fine-mapping study has identified novel loci in the KLK6 region strongly associated with aggressive PCa. PMID: 28376164
    13. mRNA expression levels of KLK6 and KLK8 in advanced serous ovarian cancer PMID: 27483364
    14. Data indicate that elevated expression of microRNA-375 in head and neck squamous cell carcinoma (HNSCC) cells significantly reduces kallikrein 6 (KLK6), kallikrein 10 (KLK10), and matrix metalloproteinase 9 (MMP9) messenger RNA expression. PMID: 26172508
    15. KLK6 expression was detected in head and neck tumor cell lines (FaDu, Cal27 and SCC25), but not in HeLa cervix carcinoma cells. PMID: 25990935
    16. The results of this study indicated that reductions in kallikrein-6 and calpain-1 may contribute to the accumulation of alpha-synuclein in DLB. PMID: 25476568
    17. KLK6 and KLK10 may be useful markers and potential therapeutic targets in gastroesophageal junction tumors PMID: 25649006
    18. This study assessed the prognostic utility of human tissue kallikrein-like peptidases 6 and 10 (KLK6 and KLK10) and correlated their expression with histopathological and clinical parameters in gastric cancer. PMID: 25153389
    19. KLK6 may represent a potential unfavorable prognostic biomarker for colon cancer. PMID: 25153391
    20. KLK6 promotes the proliferation of lung tumoral cells and restrains their apoptosis in vitro via ligand-dependent EGFR and was dependent on the protease-activated receptor 2. PMID: 24643912
    21. The level of KLK6 is significantly increased in the plasma samples from laryngeal cancer patients compared to the controls. PMID: 24669031
    22. Our data suggest that de novo KLK6 expression during early liver carcinogenesis may induce production of Ubi(1-74) by post-translational modification of ubiquitin PMID: 24979321
    23. KLK6 mRNA expression could be considered as an independent, unfavorable molecular prognostic biomarker in colorectal adenocarcinoma, with additional prognostic value in patients without regional or distant metastases PMID: 24430362
    24. Data indicate there was no SNP in KLK6 was associated with altered risk of prostate cancer. PMID: 21741862
    25. Neuropathological analysis following delivery of LV-Neurosin to alpha-syn tg mice resulted in reduced accumulation of alpha-syn and reversal of neurodegenerative alterations in wild type but not A53T alpha-syn tg mice PMID: 22508489
    26. Patients with synucleinopathy displayed lower cerebrospinal fluid levels of neurosin and alpha-synuclein compared to controls and Alzheimer's disease patients. PMID: 23308173
    27. KLK6 DNA methylation was significantly associated with pathological stage in prostate cancer patients operated by radical prostatectomy. PMID: 22874102
    28. Decreased serum concentrations of KLK6 are found in patients with aneurysmal subarachnoid hemorrhage, with the lowest levels in patients who died PMID: 23049835
    29. hsa-let-7 family member hsa-let-7a is a modulator of KLK6 protein expression that is independent of the KLK6 copy number status PMID: 23136250
    30. KLK6 gene expression may have clinical utility as a marker of unfavorable prognosis for intracranial tumors, and consequently, it could be used as target for therapeutic intervention. PMID: 22477710
    31. in colorectal cancer patients, lymph node KLK6 positivity indicated presence of aggressive tumour cells associated with poor prognosis and high risk of tumour recurrence PMID: 22699826
    32. KLK6 is positioned to serve as a molecular trigger of select physiological processes involved in the development of astrogliosis PMID: 22505518
    33. KLK6 expressed by stromal cells may considerably contribute to the aggressiveness of ovarian cancer PMID: 22505521
    34. KLK6 is significantly upregulated and secreted in gastric cancer tissues and sera, suggesting that KLK6 might be used as a potential biomarker and therapeutic target for gastric cancer. PMID: 22373580
    35. KLK6 immunostaining is an independent prognostic marker in patients with colorectal carcinoma. PMID: 22285222
    36. High KLK6 is associated with ovarian cancer. PMID: 22102857
    37. KLK6-induced intracellular Ca(2+) flux and tumor cell invasion critically depends on the protease-activated receptor 1 (PAR1). PMID: 21753781
    38. KLK6 has a role in tumour proliferation and progression of NSCLC PMID: 19426157
    39. upregulated in late stage epithelial ovarian cancer PMID: 20680316
    40. KLK6 was up-regulated significantly in tissues and sera from patients with colon cancer and was associated closely with a poor prognosis. PMID: 21656738
    41. KLK6 promotes lymphocyte survival by a mechanism that depends in part on activation of PAR1. PMID: 21464892
    42. The expression of kallikrein 7 and kallikrein 6 in melanomas may be responsible for the loss of cell-cell adhesion. PMID: 21193224
    43. an altered KLK6 expression may contribute to vascular abnormalities in Alzheimer's disease and vascular dementia PMID: 20846516
    44. Neurosin targets extracellular alpha-synuclein in cultured cells. PMID: 20403393
    45. Patients diagnosed with advanced multiple sclerosis showed mean CSF levels of kallikrein-related peptidase 6 (29 ng/ml) that were significantly higher than in neurological controls (25.5 ng/ml). PMID: 20836755
    46. The expression of hK6 in ovarian cancer was higher than that in benign and borderline ovarian neoplasms. Expression is higher in the ovarian cancer of late stage, higher-grade, with lymph node metastasis and is associated with a poorer prognosis. PMID: 19950700
    47. KLK6 expression in cancerous tissues may play an important role in the invasion and metastasis of primary breast carcinoma. PMID: 18992199
    48. Data show that it was unable to distinguish men with and without prostate cancer using multiple kallikreins as urinary biomarkers. PMID: 19560453
    49. characterization of human kallikrein 6 as a degradative protease with structural features more similar to trypsin than the regulatory kallikreins PMID: 11983703
    50. X-ray crystallographic structure of KLK6. PMID: 12016211

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  • 亚细胞定位：
    Secreted. Nucleus, nucleolus. Cytoplasm. Mitochondrion. Microsome. Note=In brain, detected in the nucleus of glial cells and in the nucleus and cytoplasm of neurons. Detected in the mitochondrial and microsomal fractions of HEK-293 cells and released into the cytoplasm following cell stress.
  • 蛋白家族：
    Peptidase S1 family, Kallikrein subfamily
  • 组织特异性：
    In fluids, highest levels found in milk of lactating women followed by cerebrospinal fluid, nipple aspirate fluid and breast cyst fluid. Also found in serum, seminal plasma and some amniotic fluids and breast tumor cytosolic extracts. Not detected in urin
  • 数据库链接：

    HGNC: 6367

    OMIM: 602652

    KEGG: hsa:5653

    STRING: 9606.ENSP00000309148

    UniGene: Hs.79361