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  4. Recombinant Human Killin (KLLN)

Recombinant Human Killin (KLLN)

  • 货号：
    CSB-YP456133HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP456133HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP456133HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP456133HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP456133HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    KLLN
  • Uniprot No.：
  • 别名：
    KLLN; Killin
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-178
  • 氨基酸序列
    MDRPGPGSAR PGRTVHVWGY RVEWKVRNGR KLQPSEWAGR GDLGGFKRRW KDTRATVGTT FRRRSRVSLV GELSKFPLPS DSSGGKSSSS FARGALAWCR QRNPNPSCAA AETGARTSLP KERCRGWRLG NWLHKHPHPN TCPRLPACWL PPILTERGER VPKLVPLLAC YPKSKPKD
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    DNA-binding protein involved in S phase checkpoint control-coupled apoptosis by mediating p53/TP53-induced apoptosis. Has the ability to inhibit DNA synthesis and S phase arrest coupled to apoptosis. Has affinity to both double- and single-stranded DNA.
  • 基因功能参考文献：
    1. Data suggest that low levels of PTEN and KLLN are associated with both sporadic papillary thyroid carcinoma and multi-nodular goiter (as compared to control subjects); however, PTEN and KLLN should not be considered as circulating biomarkers for differential diagnosis between malignancy and benignity in indeterminate thyroid nodules. (PTEN = phosphatase and tensin homolog; KLLN = DNA replication inhibitor killin) PMID: 28755140
    2. The results suggest a critical role for KLLN as a potential regulator of pericentric heterochromatin formation, genomic stability and gene expression. PMID: 26673699
    3. overexpression of microRNA-224 enhanced the proliferation abilities of HO8910 and knockdown of microRNA-224 suppressed the proliferation abilities of HO8910PM by KLLN-cyclin A pathway. PMID: 27663866
    4. Germline promoter hypermethylation of KLLN is associated with particular malignant and benign Cowden syndrome features, which is dependent on the PTEN mutation status. PMID: 25669429
    5. Killin has a role in competitively inhibiting the formation of DNA replication forks during S-phase, as well as potentially negatively regulate RNA synthesis during other cell cycle phases PMID: 25945611
    6. Data indicate that NVP-BEZ235-induced androgen receptor (AR) suppression resulted in decreased expression of both tumor suppressor proteins PTEN and KLLN in AR+/ER+ breast cancer cells. PMID: 24356815
    7. Nuclear KLLN expression associates with improved relapse-free survival for prostate carcinoma PMID: 24972837
    8. We demonstrate, in vitro and in murine xenograph models, that both KLLN and PTEN are AR-target genes, mediating androgen-induced growth inhibition and apoptosis in breast cancer cells. PMID: 23418309
    9. Germline KLLN mutations dysregulate the cell cycle at G2. PMID: 23446638
    10. KLLN is a transcription factor directly regulating AR, TP53, and TP73 expression, with a role in prostate carcinogenesis. PMID: 23386643
    11. Pathogenic mutations in KLLN are rare in breast cancer families and the c.339_340delAG variant does not represent a high-penetrance breast cancer risk allele. PMID: 22580995
    12. Cowden syndrome patients have elevated risks of papillary thyroid cancer from KLLN alterations. PMID: 21956414
    13. 19 of 20 advanced RCC showed somatic hypermethylation upstream of KILLIN PMID: 21584899
    14. Germline KILLIN methylation is common among patients with Cowden syndrome or Cowden-like syndrome and is associated with increased risks of breast and renal cancer over PTEN mutation-positive individuals PMID: 21177507
    15. results support Killin as a missing link between p53 activation and S phase checkpoint control designed to eliminate replicating precancerous cells, should they escape G(1) blockade mediated by p21. PMID: 18385383
    16. Killin is a p53-regulated nuclear inhibitor of DNA synthesis with high affinity of DNA binding. PMID: 18385383

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  • 相关疾病：
    Cowden syndrome 4 (CWS4)
  • 亚细胞定位：
    Nucleus.
  • 数据库链接：

    HGNC: 37212

    OMIM: 612105

    KEGG: hsa:100144748

    STRING: 9606.ENSP00000392204

    UniGene: Hs.559820