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  4. Recombinant Human L-amino-acid oxidase (IL4I1)

Recombinant Human L-amino-acid oxidase (IL4I1)

  • 中文名称：
    人IL4I1重组蛋白
  • 货号：
    CSB-YP850426HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人IL4I1重组蛋白
  • 货号：
    CSB-EP850426HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人IL4I1重组蛋白
  • 货号：
    CSB-EP850426HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人IL4I1重组蛋白
  • 货号：
    CSB-BP850426HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人IL4I1重组蛋白
  • 货号：
    CSB-MP850426HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    IL4I1
  • Uniprot No.：
  • 别名：
    IL4I1; FIG1; UNQ636/PRO1265; L-amino-acid oxidase; LAAO; LAO; EC 1.4.3.2; Interleukin-4-induced protein 1; IL4-induced protein 1; Protein Fig-1; hFIG1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    22-567
  • 氨基酸序列
    QDWKAERSQ DPFEKCMQDP DYEQLLKVVT WGLNRTLKPQ RVIVVGAGVA GLVAAKVLSD AGHKVTILEA DNRIGGRIFT YRDQNTGWIG ELGAMRMPSS HRILHKLCQG LGLNLTKFTQ YDKNTWTEVH EVKLRNYVVE KVPEKLGYAL RPQEKGHSPE DIYQMALNQA LKDLKALGCR KAMKKFERHT LLEYLLGEGN LSRPAVQLLG DVMSEDGFFY LSFAEALRAH SCLSDRLQYS RIVGGWDLLP RALLSSLSGL VLLNAPVVAM TQGPHDVHVQ IETSPPARNL KVLKADVVLL TASGPAVKRI TFSPPLPRHM QEALRRLHYV PATKVFLSFR RPFWREEHIE GGHSNTDRPS RMIFYPPPRE GALLLASYTW SDAAAAFAGL SREEALRLAL DDVAALHGPV VRQLWDGTGV VKRWAEDQHS QGGFVVQPPA LWQTEKDDWT VPYGRIYFAG EHTAYPHGWV ETAVKSALRA AIKINSRKGP ASDTASPEGH ASDMEGQGHV HGVASSPSHD LAKEEGSHPP VQGQLSLQNT THTRTSH
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Secreted L-amino-acid oxidase that acts as a key immunoregulator. Has preference for L-aromatic amino acids: converts phenylalanine (Phe), tyrosine (Tyr) and tryptophan (Trp) to phenylpyruvic acid (PP), hydroxyphenylpyruvic acid (HPP), and indole-3-pyruvic acid (I3P), respectively. Also has weak L-arginine oxidase activity. Acts as a negative regulator of anti-tumor immunity by mediating Trp degradation via an indole pyruvate pathway that activates the transcription factor AHR. IL4I1-mediated Trp catabolism generates I3P, giving rise to indole metabolites (indole-3-acetic acid (IAA) and indole-3-aldehyde (I3A)) and kynurenic acid, which act as ligands for AHR, a ligand-activated transcription factor that plays important roles in immunity and cancer. AHR activation by indoles following IL4I1-mediated Trp degradation enhances tumor progression by promoting cancer cell motility and suppressing adaptive immunity. Also has an immunoregulatory function in some immune cells, probably by mediating Trp degradation and promoting downstream AHR activation: inhibits T-cell activation and proliferation, promotes the differentiation of naive CD4(+) T-cells into FOXP3(+) regulatory T-cells (Treg) and regulates the development and function of B-cells. Also regulates M2 macrophage polarization by inhibiting T-cell activation. Also has antibacterial properties by inhibiting growth of Gram negative and Gram positive bacteria through the production of NH4(+) and H2O2.
  • 基因功能参考文献：
    1. the presence of IL4I1 during T-cell activation decreases early signaling events downstream of t-cell receptor stimulation, resulting in global T-cell inhibition which is more pronounced when there is CD28 costimulation. PMID: 28891065
    2. Data showed proteome changes in alveolar epithelial cells type II exposed to an infection with A. fumigatus with IL4I1 showing the most prominent increase in abundance both, in terms of relative abundance as well as activity. Also, in the infected lungs, there was levels of IL4I1 metabolic products. PMID: 28951444
    3. reviewed SNPs of the IL4I1 isoform 1, which is expressed in lymphoid tissue; the N92D SNP leads to a hyperactive enzyme, while the R102G mutation is hypomorphic; show that IL4I1 activity is not only directed against phenylalanine, as initially described, but also at a lower level against arginine PMID: 26673964
    4. The immunosuppressive enzyme IL4I1 expressed differentially in human induced Aiolos+, but not natural Helios+, FOXP3+ Treg cells. PMID: 25446972
    5. IL4I1 upregulation in human Th17 cells limits their TCR-mediated expansion not only by blocking the molecular pathway involved in the activation of the IL-2 promoter, but also by maintaining high levels of Tob1, which impairs entry into the cell cycle. PMID: 24307243
    6. IL4I1 plays a distinct role compared to other antibacterial enzymes produced by mononuclear phagocytes. PMID: 23355881
    7. participates in the downregulation of Th1 inflammation PMID: 20683900
    8. genomic structure, cDNA sequence, chromosome location and RNA expression in immune tissues PMID: 12031486
    9. is activated in primary mediastinal large B-cell lymphoma, which might be due to a constitutive activation of a cytokine signaling pathway PMID: 12446450
    10. hIL4I1 inhibited the proliferation of CD3-stimulated T lymphocytes with a similar effect on CD4(+) and CD8(+) T cells. In contrast, memory T cells were more strongly affected by hIL4I1 and its catabolite H(2)O(2) than naive T cells PMID: 17356132
    11. strong IL4I1 expression is associated with B-cell lymphomas. PMID: 19436310

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  • 亚细胞定位：
    Secreted. Lysosome. Cytoplasmic vesicle, secretory vesicle, acrosome.
  • 蛋白家族：
    Flavin monoamine oxidase family, FIG1 subfamily
  • 组织特异性：
    Primarily found in immune tissues, with the highest expression in lymph nodes and spleen. Present in germinal center macrophages and inflammatory myeloid cells and antigen-presenting cells (at protein level). Also present in spermatozoa (at protein level)
  • 数据库链接：

    HGNC: 19094

    OMIM: 609742

    KEGG: hsa:259307

    STRING: 9606.ENSP00000342557

    UniGene: Hs.574492