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  4. Recombinant Human LIM domain and actin-binding protein 1 (LIMA1)

Recombinant Human LIM domain and actin-binding protein 1 (LIMA1)

  • 中文名称：
    人LIMA1重组蛋白
  • 货号：
    CSB-YP883388HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人LIMA1重组蛋白
  • 货号：
    CSB-EP883388HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人LIMA1重组蛋白
  • 货号：
    CSB-EP883388HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人LIMA1重组蛋白
  • 货号：
    CSB-BP883388HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人LIMA1重组蛋白
  • 货号：
    CSB-MP883388HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    LIMA1
  • Uniprot No.：
  • 别名：
    1110021C24Rik; Epithelial protein lost in neoplasm; Epithelial protein lost in neoplasm beta; EPLIN; FLJ38853; LIM domain and actin binding 1; LIM domain and actin binding protein 1 ; LIM domain and actin-binding protein 1; LIMA1; LIMA1_HUMAN; MGC131726; SREBP3; Sterol regulatory element binding protein 3
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-759
  • 氨基酸序列
    MESSPFNRRQ WTSLSLRVTA KELSLVNKNK SSAIVEIFSK YQKAAEETNM EKKRSNTENL SQHFRKGTLT VLKKKWENPG LGAESHTDSL RNSSTEIRHR ADHPPAEVTS HAASGAKADQ EEQIHPRSRL RSPPEALVQG RYPHIKDGED LKDHSTESKK MENCLGESRH EVEKSEISEN TDASGKIEKY NVPLNRLKMM FEKGEPTQTK ILRAQSRSAS GRKISENSYS LDDLEIGPGQ LSSSTFDSEK NESRRNLELP RLSETSIKDR MAKYQAAVSK QSSSTNYTNE LKASGGEIKI HKMEQKENVP PGPEVCITHQ EGEKISANEN SLAVRSTPAE DDSRDSQVKS EVQQPVHPKP LSPDSRASSL SESSPPKAMK KFQAPARETC VECQKTVYPM ERLLANQQVF HISCFRCSYC NNKLSLGTYA SLHGRIYCKP HFNQLFKSKG NYDEGFGHRP HKDLWASKNE NEEILERPAQ LANARETPHS PGVEDAPIAK VGVLAASMEA KASSQQEKED KPAETKKLRI AWPPPTELGS SGSALEEGIK MSKPKWPPED EISKPEVPED VDLDLKKLRR SSSLKERSRP FTVAASFQST SVKSPKTVSP PIRKGWSMSE QSEESVGGRV AERKQVENAK ASKKNGNVGK TTWQNKESKG ETGKRSKEGH SLEMENENLV ENGADSDEDD NSFLKQQSPQ EPKSLNWSSF VDNTFAEEFT TQNQKSQDVE LWEGEVVKEL SVEEQIKRNR YYDEDEDEE
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Actin-binding protein involved in actin cytoskeleton regulation and dynamics. Increases the number and size of actin stress fibers and inhibits membrane ruffling. Inhibits actin filament depolymerization. Bundles actin filaments, delays filament nucleation and reduces formation of branched filaments. Plays a role in cholesterol homeostasis. Influences plasma cholesterol levels through regulation of intestinal cholesterol absorption. May act as a scaffold protein by regulating NPC1L1 transportation, an essential protein for cholesterol absorption, to the plasma membrane by recruiting MYO5B to NPC1L1, and thus facilitates cholesterol uptake.
  • 基因功能参考文献：
    1. this study identifies LIMA1 as a key protein regulating intestinal cholesterol absorption. PMID: 29880681
    2. Reduction in the levels of hCDC14A and eplin mRNA is frequently associated with colorectal carcinoma and is correlated with poor prognosis. s therefore propose that eplin dephosphorylation by hCDC14A reduces actin dynamics to restrict tumor malignancy. PMID: 28465438
    3. Data provide evidence that downregulation of EPLIN-alpha may be associated with poor prognosis for patients with epithelial ovarian cancer (EOC), and that this molecule appears to play a tumour suppressor role by inhibition of EOC growth and migration. PMID: 27035883
    4. EPLIN is functionally linked to molecules like actin and paxillin and has been implicated in a number of potential pathways to enhance metastatic potential PMID: 26350886
    5. a major activity of DNp73 is to establish initiation of the invasion-metastasis cascade via EPLIN-dependent IGF1R regulation PMID: 24135282
    6. EGF promotes epithelial-mesenchymal transition and induces degradation of EPLIN, a putative suppressor of prostate cancer metastasis. PMID: 23188829
    7. Together with the findings that EPLIN-alpha inhibits cellular growth and invasion, we conclude that EPLIN-alpha is a tumour suppressor of oesophageal cancer PMID: 22493360
    8. EPLIN clutch is necessary for stabilization of capillary structures in an angiogenesis model. PMID: 22194609
    9. EPLIN downregulation promotes epithelial-mesenchymal transition in prostate cancer cells and correlates with clinical lymph node metastasis. PMID: 21625216
    10. EPLINalpha over-expression can regulate HECV cell motility, matrix adhesion and tubule formation in vitro and slow in vivo tumour formation, suggesting an anti-angiogenic role for EPLINalpha. PMID: 20848180
    11. EPLIN functions to link the cadherin-catenin complex to F-actin and simultaneously stabilizes this population of actin fibers, resulting in the establishment of the adhesion PMID: 18093941
    12. expression of EPLIN-alpha in breast cancer is down-regulated in breast cancer cells . PMID: 18796137
    13. EPLIN protein may function during cytokinesis to maintain local accumulation of key cytokinesis proteins at the furrow. PMID: 19221476

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  • 亚细胞定位：
    Cytoplasm. Cell junction, focal adhesion. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, stress fiber. Cell membrane.
  • 组织特异性：
    Highly expressed in placenta, kidney, pancreas, prostate, ovary, spleen and heart. Also detected in lung, liver, brain, skeletal muscle, thymus, testis and intestine. Not detected in leukocytes. Isoform Beta expressed generally at very low levels. Isoform
  • 数据库链接：

    HGNC: 24636

    OMIM: 608364

    KEGG: hsa:51474

    STRING: 9606.ENSP00000378400

    UniGene: Hs.525419