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  4. Recombinant Human Lactadherin (MFGE8)

Recombinant Human Lactadherin (MFGE8)

  • 货号：
    013752HU
  • 规格：
  • 来源：
  • 其他：
  • 货号：
    CSB-EP013752HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP013752HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP013752HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    BA46; Breast epithelial antigen BA46; EDIL1; HMFG; hP47; HsT19888; Lactadherin; Medin; MFG-E8; MFGE8; MFGM; MFGM_HUMAN; Milk fat globule EGF factor 8; Milk fat globule EGF factor 8 protein; Milk fat globule-EGF factor 8; O acetyl disialoganglioside synthase; OAcGD3S; SED1; SPAG10; Sperm associated antigen 10; Sperm surface protein hP47
  • 表达区域：
    24-387
  • 氨基酸序列
    LDICSKN PCHNGGLCEE ISQEVRGDVF PSYTCTCLKG YAGNHCETKC VEPLGMENGN IANSQIAASS VRVTFLGLQH WVPELARLNR AGMVNAWTPS SNDDNPWIQV NLLRRMWVTG VVTQGASRLA SHEYLKAFKV AYSLNGHEFD FIHDVNKKHK EFVGNWNKNA VHVNLFETPV EAQYVRLYPT SCHTACTLRF ELLGCELNGC ANPLGLKNNS IPDKQITASS SYKTWGLHLF SWNPSYARLD KQGNFNAWVA GSYGNDQWLQ VDLGSSKEVT GIITQGARNF GSVQFVASYK VAYSNDSANW TEYQDPRTGS SKIFPGNWDN HSHKKNLFET PILARYVRIL PVAWHNRIAL RLELLGC
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization. Contributes to phagocytic removal of apoptotic cells in many tissues. Specific ligand for the alpha-v/beta-3 and alpha-v/beta-5 receptors. Also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction.; Main constituent of aortic medial amyloid.
  • 基因功能参考文献：
    1. Esophageal cancer patients with high MFG-E8 expression had worse overall survival and relapse-free survival in the subgroup with preoperative chemotherapy. The abundant MFG-E8 expression in esophageal squamous cell carcinoma might have a negative influence on the long-term survival of patients after chemotherapy by affecting T-cell regulation in the tumor microenvironment. PMID: 30156356
    2. MFGE8 polymorphisms were not associated with MetS but were related to DBP, HDL-C level, and WC in Chinese adults. PMID: 30053459
    3. This study showed for the first time that MFG-E8 expression is impaired in the endometrium of patients with endometriosis and infertility during the window of implantation. PMID: 28967712
    4. Our findings suggest that both MFGE8 and TGFbeta1 are agerelated inflammatory factors and are related to the degree of Atherosclerosis (AS). In conclusion, both MFGE8 and TGFbeta1 may serve as potential markers of the severity of AS PMID: 29152651
    5. Elevated serum MFG-E8 levels may be associated with cerebrovascular diseases or neuropsychiatric systemic lupus erythematosus. PMID: 28266034
    6. Circulating MFG-E8 levels are dramatically elevated in pregnancy, and are significantly higher in gestational diabetes mellitus than in normal pregnancy. PMID: 28035763
    7. MFG-E8 could be a potential novel prognostic marker for colorectal cancer (CRC) and overexpression of MFG-E8 might be involved in lymph node metastasis and angiogenesis of CRC. PMID: 28923329
    8. MFG-E8 levels are elevated in T2D but suppressed by increased adipose tissues, thereby allowing inflammatory factors to rise to high levels. MFG-E8 may serve as a potential biomarker for obesity and T2D in the clinical setting PMID: 28067023
    9. These findings provide a better understanding of the molecular mechanism underlying colorectal cancer progression and suggest a predictive role for MFG-E8 in colorectal cancer metastasis and prognosis PMID: 28653875
    10. Levels of MFGE8 were reduced in cirrhotic liver tissue from patients compared with controls. PMID: 27956229
    11. an efficient purification method for production of non-aggregated, full-length MFG-E8. PMID: 27102803
    12. Reduced serum milk fat globule-epidermal growth factor 8 (MFG-E8) concentrations are associated with an increased risk of microvascular complications in patients with type 2 diabetes. PMID: 28089751
    13. MFG-E8 is a protective factor in the pathogenesis of rheumatoid arthritis and subsequent bone loss. PMID: 26391522
    14. After myocardial infarction, Mfge8 (and Mertk)-expressing macrophages synergistically engage the clearance of injured cardiomyocytes. PMID: 26819373
    15. Studied the therapeutic effect of rhMFG-E8 in mouse models of IBD. Treatment with rhMFG-E8 significantly attenuated colitis in both models in a dose-dependent way. PMID: 25751740
    16. MFG-E8 promotes cutaneous wound healing by enhancing angiogenesis. PMID: 24838098
    17. Lactadherin may decrease inflammation by inhibiting secretory phospholipase A2 activity on pre-apoptotic leukemia cells. PMID: 24194865
    18. promotes tumor progression in oral squamous cell carcinoma PMID: 25264705
    19. MFG-E8 had a negative association with hs-CRP and a positive association with LDL-c. The serum level of MFG-E8 was negatively associated with the severity of coronary artery stenosis and the risk of clinical events. PMID: 24561551
    20. medin adopts a predominantly beta-sheet conformation with some unstructured elements. PMID: 24602872
    21. MFG-E8 could be used as a biomarker for diagnosis and monitoring of disease activity in certain systemic lupus erythematosus patients PMID: 24554711
    22. Exogenously added MFG-E8 inhibits receptor activator NF-kappaB ligand-induced osteoclastogenesis of human osteoclast precursors. PMID: 24958900
    23. Our results strongly suggested that MFG-E8 is a promising biomarker for the diagnosis, prognosis, and therapy target of opisthorchiasis-associated cholangiocarcinoma PMID: 24122204
    24. Blockage of MFG-E8 in endometrial tumor cells diminishes trophoblaast cell attachment. PMID: 24424369
    25. TNF-alpha up-regulates endometrial epithelial cell migration and MFG-E8 production. PMID: 24262600
    26. MFG-E8-dependent promotion of apoptotic cell clearance is a novel anti-inflammatory facet of glucocorticoid treatment PMID: 23832117
    27. our data argue that MFGE8 is not likely involved in the phagocytic clearance of neuronal debris associated with nigrostriatal pathway injury. PMID: 23194669
    28. NMR solution structure of C2 domain of MFG-E8 and insights into its molecular recognition with phosphatidylserine PMID: 23262193
    29. Milk fat globule-epidermal growth factor 8 has proapoptotic activity, suggesting participation in endometrial remodeling via an epithelial-stromal cell paracrine effect. PMID: 22921913
    30. MFG-E8 expression in the endometrial epithelium as well as in chorionic villi suggests its possible role in endometrial reorganization during the receptive phase and in events related to normal pregnancy in mammals PMID: 22770563
    31. Decreased colonic MFGE8 expression in patients with ulcerative colitis may be associated with mucosal inflammatory activity and clinical disease activity through basal cell apoptosis and preventing tissue healing in the pathogenesis of ulcerative colitis PMID: 22204000
    32. a key role of MFG-E8 release from apoptotic endothelial cells in macrophage reprogramming PMID: 22558449
    33. The distribution of the SNP (rs4945 and rs1878326) of MFGE8 was analyzed in two groups of patients with "wet" age-related macular Degeneration and their age-matched controls. PMID: 22438901
    34. serum lactadherin is correlated with poor blood glucose control and diabetic vascular complications. PMID: 22018779
    35. We conclude that MFG-E8-dependent signaling stimulates cell proliferation and the acquisition of mesenchymal properties and contributes to mammary carcinoma development. PMID: 21841820
    36. in vitro studies showed that medin amyloid-like fibrils promote the aggregation of protein amyloid A into fibrils PMID: 22070546
    37. Prolactin has a modulatory role for as a stromal/epithelial paracrine factor controlling MFG-E8. This is the first report on MFG-E8 protein localization to the human endometrial epithelium and its up-regulation during the window of implantation. PMID: 21177637
    38. MFG-E8 is expressed in triple-negative breast cancers as a target gene of the p63 pathway, but may serve a suppressive function in ER(+) and erbB2(+) breast cancers. PMID: 21127199
    39. overexpression of MFGE8 during bladder tumor development, correlated with expression of genes involved in cell adhesion or migration and in immune responses. PMID: 20956946
    40. intronic mutation in the human MFG-E8 gene can lead to the development of SLE. PMID: 20213738
    41. SED1 is expressed on the surface of acrosome-intact human sperm and in the anterior caput of the human epididymis, similar to that seen in mouse PMID: 18990388
    42. Truncated fragment of medin, the hexapeptide, NFGSVQ can form typical amyloid fibrils. PMID: 15478463
    43. Might prove useful in the treatment of prolonged ischemia. PMID: 16115445
    44. Lactadherin) is expressed in normal and atherosclerotic human arteries. PMID: 17420351
    45. The trans-activator (TA) isoforms of p63 activate MFGE8 transcription though a p53/p63 motif at -370, which was confirmed by a chromatin immunoprecipitation experiment. PMID: 17637751
    46. Identification of the last 18-19 amino acid residues as constituting the amyloid-promoting region of medin. PMID: 17679143
    47. aggregated medin induced death of aortic smooth muscle cells in vitro. Cells incubated together with medin increased the production of matrix metalloproteinase-2, a protease that degrades elastin and collagen and subsequently weakens the vessel wall. PMID: 17906662
    48. analysis of membrane-interactive loops of Lact-C2 PMID: 18160406
    49. Some childhood-onset and adult SLE patients carried a significant level of MFG-E8 in their blood samples. PMID: 18303131
    50. Lactadherin promoted phagocytosis of phosphatidylserine-expressing RBCs by macrophages in a concentration-dependent manner. PMID: 18647368

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  • 亚细胞定位：
    Membrane; Peripheral membrane protein. Secreted. Cytoplasmic vesicle, secretory vesicle, acrosome membrane; Peripheral membrane protein.
  • 组织特异性：
    Mammary epithelial cell surfaces and aortic media. Overexpressed in several carcinomas.
  • 数据库链接：

    HGNC: 7036

    OMIM: 602281

    KEGG: hsa:4240

    STRING: 9606.ENSP00000268150

    UniGene: Hs.3745