Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Human Lactosylceramide 4-alpha-galactosyltransferase (A4GALT), partial

Recombinant Human Lactosylceramide 4-alpha-galactosyltransferase (A4GALT), partial

  • 货号：
    CSB-YP868276HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP868276HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP868276HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP868276HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP868276HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    A4GALT
  • Uniprot No.：
  • 别名：
    4 N acetylglucosaminyltransferase; 4-galactosyltransferase; 4-N-acetylglucosaminyltransferase; A14GALT; A4GALT; A4GALT1; A4GAT_HUMAN; Alpha 1 4 galactosyltransferase; Alpha 1 4 N acetylglucosaminyltransferase; Alpha-1; Alpha4Gal T1; Alpha4Gal-T1; CD77 synthase; Gb3 synthase; Gb3S; Globotriaosylceramide synthase; Lactosylceramide 4 alpha galactosyltransferase; Lactosylceramide 4-alpha-galactosyltransferase; P blood group (P one antigen); P(k) antigen synthase; P1; P1/Pk synthase; PK; UDP galactose beta D galactosyl beta1 R 4 alpha D galactosyltransferase; UDP-galactose:beta-D-galactosyl-beta1-R 4-alpha-D-galactosyltransferase
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the transfer of galactose from UDP-alpha-D-galactose to lactosylceramide/beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide(d18:1(4E)) to produce globotriaosylceramide/globoside Gb3Cer (d18:1(4E)). Also able to transfer galactose to galactosylceramide/beta-D-Gal-(1<->1')-Cer. Globoside Gb3Cer is a glycosphingolipid of the globo serie, one of the major types of neutral root structures of glycosphingolipids, that constitute a significant portion of mammalian cell membranes. Globotriaosylceramide/globoside Gb3Cer in blood and tissue cell membranes is the antigen Pk of blood histogroup P.; (Microbial infection) Globotriaosylceramide is one of the cellular ligands for bacterial verotoxins.
  • 基因功能参考文献：
    1. The differential binding of transcription factor early growth response 1 to the SNP rs5751348 genomic region with the different genotypes in the A4GALT gene leads to differential activation of P(1) -A4GALT and P(2) -A4GALT expression. PMID: 29399809
    2. A4GALT SNPs spur extra products of the human Gb3/CD77 synthase and underlie the P1PK blood group system PMID: 29709005
    3. Study reports a novel mutation in the A4GALT gene that was responsible for a p phenotype in a Chinese individual. PMID: 27612185
    4. amino acid residue at position 211 of Gb3/CD77 synthase is critical for specificity and activity of the enzyme involved in formation of P(k), P1 and NOR antigens. Altogether, this approach affords a new insight into the mechanism of action of the human Gb3/CD77 synthase. PMID: 27538840
    5. This is the first direct biochemical evidence that Gb3/CD77 synthase is able to synthesize two different glycosphingolipid antigens: P(k) and P1, and when p.Q211E substitution is present, the NOR antigen is also synthesized. PMID: 26773500
    6. Mutation in the A4GALT gene is associated with rare p phenotype in P1Pk blood group system. PMID: 25863098
    7. The results of this investigation demonstrate a consistent association of A4GALT SNPs rs2143918 and rs5751348 with the P1/P2 phenotypes and suggest that SNP rs5751348 may lead to allelic variations in A4GALT gene expression PMID: 25041587
    8. these data elucidate a new explanation underlying the p phenotype, implicating the deleted regions of A4GALT as crucial for P1 and P(k) synthesis, possibly due to loss of binding sites for erythroid transcription factors. PMID: 24417201
    9. P1-decorated PSGL-1/mIgG2b bound with high avidity to both Stx1 and Stx2, and as such constitutes a potential therapeutic inhibitor of these toxins. PMID: 24082034
    10. several P1PK-null alleles were identified. PMID: 23927681
    11. Results suggest that the C631G mutation alters the acceptor specificity of Gb3/CD77 synthase, rendering it able to catalyze synthesis of the Gal(alpha1-4)Gal and Gal(alpha1-4)GalNAc moieties. PMID: 22965229
    12. Increased globotriaosylceramide levels are detected in a transgenic mouse expressing human alpha1,4-galactosyltransferase in a mouse model for treating Fabry disease. PMID: 20961863
    13. 2 Taiwanese individuals with p phenotype were homozygous for a & 428. No expression of P(k) & no enzyme activity were observed in cells transfected with the mutant construct. PMID: 21092013
    14. Total urinary protein isoforms of globotriaosylceramide were used to identify Fabry disease in women. PMID: 21186071
    15. Data showed that GRINA-C was associated with Gb3 synthase, and may demonstrate a new type of posttranscriptional regulation of glycosyltransferases. PMID: 20837469
    16. of A4GALT mRNA in cultured human bone marrow cells revealed novel transcripts containing only the noncoding exon 1 and a sequence (here termed exon 2a) from intron 1. PMID: 20971946
    17. We established by quantitative real-time PCR that both fresh colonic epithelial sections and HCT-8 cells express Gb3 synthase mRNA and the alternate Shiga toxin receptor globotetraosylceramide. PMID: 20732996
    18. expression in megakaryoblastic leukemia cells PMID: 11782470
    19. The P blood group phenotype is due to several disctint nonfunctional alleles of A4GALT without any predominant allele. PMID: 11896312
    20. results strongly suggested that P1 synthase is identical to Gb3/CD77 synthase PMID: 12888565
    21. Data show that a novel A4GALT missense mutation causes the p phenotype in Amish individuals. PMID: 18067504
    22. the transcriptional regulation of the Gb3S gene by Sp1 might affect the verotoxin sensitivity of endothelial cell and Hemolytic uremic syndrome progression. PMID: 18757779
    23. P(k) expression strongly influences susceptibility to HIV-1 infection, which implicates P(k) as a new endogenous cell-surface factor that may provide protection against HIV-1 infection. PMID: 19139081
    24. These data show that globotriaosylceramide (Gb3) content, which is regulated by the expression of Gb3 synthase, determines the sensitivity of HeLa cells toward Shiga toxin. PMID: 19470247

    显示更多

    收起更多

  • 亚细胞定位：
    Golgi apparatus membrane; Single-pass type II membrane protein.
  • 蛋白家族：
    Glycosyltransferase 32 family
  • 组织特异性：
    Ubiquitous. Highly expressed in kidney, heart, spleen, liver, testis and placenta.
  • 数据库链接：

    HGNC: 18149

    OMIM: 111400

    KEGG: hsa:53947

    STRING: 9606.ENSP00000249005

    UniGene: Hs.105956