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  4. Recombinant Human Lymphocyte antigen 96 (LY96) (R56G)

Recombinant Human Lymphocyte antigen 96 (LY96) (R56G)

In Stock
  • 货号：
    CSB-EP013254HU(M)
  • 规格：
    ￥1344
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    ESOP 1; ESOP-1; ESOP1 ; LY 96; Ly-96; LY96; LY96_HUMAN; Lymphocyte antigen 96; md 2; MD 2 protein; MD2 protein; Myeloid differentiation protein 2 ; Protein MD 2; Protein MD-2; Protein MD2
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    E.coli
  • 分子量：
    20.0 kDa
  • 表达区域：
    19-160aa(R56G)
  • 氨基酸序列
    QKQYWVCNSSDASISYTYCDKMQYPISINVNPCIELKGSKGLLHIFYIPRRDLKQLYFNLYITVNTMNLPKRKEVICRGSDDDYSFCRALKGETVNTTISFSFKGIKFSKGKYKCVVEAISGSPEEMLFCLEFVILHQPNSN
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer，50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Binds bacterial lipopolysaccharide (LPS). Cooperates with TLR4 in the innate immune response to bacterial lipopolysaccharide (LPS), and with TLR2 in the response to cell wall components from Gram-positive and Gram-negative bacteria. Enhances TLR4-dependent activation of NF-kappa-B. Cells expressing both LY96 and TLR4, but not TLR4 alone, respond to LPS.
  • 基因功能参考文献：
    1. sTLR4/MD-2 complex inhibits colorectal cancer migration and invasiveness in vitro and in vivo by lncRNA H19 down-regulation. PMID: 29036538
    2. MD-2 siRNA or plasmid further confirmed the efficacy of Iturin A by suppressing MD-2/TLR4 signaling pathway. The in silico docking study showed that the Iturin A interacted well with the MD-2 in MD-2/TLR4 receptor complex. Conclusively, inhibition of MD-2/TLR4 complex with Iturin A offered strategic advancement in cancer therapy. PMID: 28347875
    3. data confirm that engineered human cells expressing TLR4, MD2 and CD14 can respond to CMP with NF-kappaB activation and the response can be influenced by variations in CMP-mannosylation PMID: 29281684
    4. The observations suggest that MD-2 helps to regulate lipopolysaccharide-induced NLRP3 inflammasome activation and the inflammatory response in NR8383 cells, and likely does so by affecting MyD88/NF-kappaB signaling. PMID: 28654770
    5. MD2 plays an important role in induction of allergic sensitization to cat dander and common pollens relevant to human allergic diseases. PMID: 26586036
    6. we report that exogenous CnB is taken up by cells in a time- and concentration-dependent manner via clathrin-dependent receptor-mediated internalization. Our findings further confirm that uptake is mediated by the TLR4/MD2 complex together with the co-receptor CD14 PMID: 27090571
    7. In this study, a novel naturally occurring spliceosome of human MD2, termed as MD2-T3, has been identified. PMID: 27317890
    8. Results show that cigarette smoke may alter innate immune responses reducing the expression of the MD2, a molecule with an important role in TLR4 signaling. PMID: 26068048
    9. Predominantly hydrophobic interactions between MD-2 and TLR4 contribute to the stabilization of the TLR4/MD-2/metal ion complex in a conformation that enables activation. PMID: 25803856
    10. The intensity of the intra-amniotic inflammatory response to bacteria or perhaps to other TLR4 ligands may be facilitated through synthesis and release of sMD2 by the amniochorion. PMID: 25605324
    11. Three genes (LY96, IL8 DPR) were significantly downregulated over time. This finding was confirmed in a validation cohort of stroke patients (n=8). PMID: 25124890
    12. The study revealed the impact of specific residues and regions of MD-2 on the binding of lipolysaccharides and TLR4. PMID: 26320630
    13. Gene polymorphisms of MD2 and GM2A were associated with the occurrence or severity of neonatal necrotizing enterocolitis. PMID: 25816011
    14. In patients undergoing CABG surgery, we found genetic polymorphisms in LY96 associated with decreased risk of postoperative AF. PMID: 26385043
    15. Aminoarabinose residues in lipid A contribute to Burkholderia lipid A binding to the TLR4.Myeloid Differentiation Factor 2 complex. PMID: 26160169
    16. TLR4 along with its accessory protein MD-2 builds a heterodimeric complex that specifically recognizes lipopolysaccharides, which are present on the cell wall of gram-negative bacteria, activating the immune response. (Review) PMID: 25515751
    17. genetic polymorphism is associated with asthma exacerbations in children PMID: 24902762
    18. In monocytes of sepsis, MD-2 expression was found to be regulated by STAT1. PMID: 24858600
    19. Substitution of hMD-2 residue V82 with an amino acid residue with a bulkier hydrophobic side chain enables activation of TLR4/MD-2. PMID: 25203747
    20. This study suggests that genetic variants of LY96 may modulate the effect of smoking on carotid plaque burden. PMID: 24954085
    21. overexpression of MD-2s led to marked reduction in markers of tissue injury and inflammation in models of lung inflammation PMID: 25576596
    22. Purified monomeric ligand.MD-2 complexes reveal molecular and structural requirements for activation and antagonism of TLR4 by Gram-negative bacterial endotoxins. PMID: 24895101
    23. Studies suggest that myeloid differentiation 2 (MD-2) as a therapeutic target for sepsis. PMID: 24896325
    24. Mechanistically, engagement of MD-2 by PTX3-opsonized Aspergillus conidia activated the TLR4/Toll/IL-1R domain-containing adapter inducing IFN-beta-dependent signaling pathway converging on IL-10. PMID: 25049357
    25. Collectively, these data showed for the first time that, HIV-1 Tat interacts physically with high affinity with TLR4-MD2 to promote proinflammatory cytokines (TNF-alpha) and the immunosuppressive cytokine IL-10 both. PMID: 24165011
    26. Data suggest that zhankuic acid A (ZAA), which competes with LPS for binding to MD-2 as a TLR4/MD-2 antagonist, may be a potential therapeutic agent for gram-negative bacterial infections. PMID: 24532584
    27. these findings indicate that MD-2 is involved in IFN-gamma signalling and IFN-gamma-augmented MMP-1 up-regulation by LPS. PMID: 23800176
    28. Extracellular complex formation of TLR4 with secreted MD-2 was detectable using monoclonal antibodies. PMID: 24021278
    29. both oral and enteric C. concisus strains upregulated expression of TLR4 and MD-2 in HT-29 cells. PMID: 23437263
    30. Consistently, soluble TLR4 expressed without MD2 inhibited metal- but not LPS-induced responses, opening new therapeutic perspe PMID: 23059983
    31. Respiratory syncytial virus F protein requires MD-2 for the induction of the TLR4-mediated inflammatory response. PMID: 22872782
    32. In LOS.MD-2 complexes, one of the six fatty acyl chains of LOS is more susceptible to paramagnetic attenuation, suggesting protrusion of that fatty acyl chain from the hydrophobic pocket of MD-2, independent of association with TLR4. PMID: 22433852
    33. Lipopolysaccharide cytokine response was efficiently and equally abolished by MD-2 and CD14 neutralization; the response induced by whole E. coli bacteria was only modestly reduced by MD-2 neutralization, whereas CD14 neutralization was more efficient. PMID: 21948372
    34. have identified specific structural and dynamic features of the MD-2-endotoxin complexes that may control dimerization of TLR4 molecules. PMID: 21924775
    35. The association between occupational endotoxin exposure and wheeze in agricultural workers was significantly modified by genetic variants in CD14 and MD2 PMID: 21389010
    36. Data demonstrate that during bacterial infection, newborns amplify the TLR2-MyD88 pathway in G+ bacterial infection and the TLR4/MD2/MyD88 pathway in G- bacterial infection, implicating the innate immune pathway in response to bacterial infection. PMID: 20805788
    37. higher expression levels in tissue from patients with colorectal cancer PMID: 20699280
    38. Computational study of the interactions of the unmodified dendrimer, glucosamine, and of the partially glycosylated dendrimer with TLR4 and MD-2 using molecular docking and molecular dynamics techniques. PMID: 21738462
    39. tyrosine phosphorylation of MD-2 is important for signaling following exposure to lipopolysaccharide PMID: 21918188
    40. Cytokines can enhance TLR4 and MD-2 expressions and promote the reaction with LPS in intestinal epithelial cells. PMID: 20848773
    41. Interferon-gamma-induced MD-2 protein expression and lipopolysaccharide (LPS) responsiveness in corneal epithelial cells is mediated by Janus tyrosine kinase-2 activation and direct binding of STAT1 protein to the MD-2 promoter. PMID: 21572044
    42. In this study, we produced variants of MD-1 and MD-2 in Pichia pastoris. PMID: 21130168
    43. Innate immune recognition of meningococcal capsular polysaccharides by macrophages can occur via TLR2- and TLR4-MD-2 pathways. PMID: 21191086
    44. Neisserial lipooligosaccharides from different meningococcal and gonococcal strains have different potencies to activate NF-kappaappaB through the Toll-like receptor 4-MD-2 pathway in monocytes. PMID: 21037101
    45. Data conclude that these gene variants in the MD-2 gene promoter region are not associated with tuberculosis, and apparently do not play a role in susceptibility to tuberculosis in the Chinese population. PMID: 20730709
    46. species-specific activation of lipid IV(A) PMID: 20592019
    47. increased expression in intestinal epithelial cells in patients with inflammatory bowel disease PMID: 19710105
    48. In this study, we have identified a novel alternatively spliced isoform of human MD-2, termed MD-2 short (MD-2s), which lacks the region encoded by exon 2 of the MD-2 gene. PMID: 20435923
    49. Polymorphisms of the promoter region of MD-2 gene at position - 1625 C/G is correlated with MODS and sepsis after severe trauma in Chinese Han population. PMID: 19209789
    50. sMD-2 and sCD14 inhibit the growth of both Gram-positive and Gram-negative bacteria. PMID: 20377740

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  • 亚细胞定位：
    Secreted, extracellular space. Secreted.
  • 数据库链接：

    HGNC: 17156

    OMIM: 605243

    KEGG: hsa:23643

    STRING: 9606.ENSP00000284818

    UniGene: Hs.726603