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  4. Recombinant Human MAP/microtubule affinity-regulating kinase 4 (MARK4)

Recombinant Human MAP/microtubule affinity-regulating kinase 4 (MARK4)

  • 货号：
    CSB-YP013499HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP013499HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP013499HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP013499HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP013499HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    MARK4
  • Uniprot No.：
  • 别名：
    FLJ90097; KIAA1860; MAP / microtubule affinity regulating kinase 4; MAP / microtubule affinity regulating kinase 4L; MAP/microtubule affinity regulating kinase 4; MAP/microtubule affinity regulating kinase like 1; MAP/microtubule affinity-regulating kinase 4; MAP/microtubule affinity-regulating kinase-like 1; MARK 4; MARK 4L; MARK L1; MARK4; MARK4 serine / threonine protein kinase; MARK4 serine/threonine protein kinase; MARK4_HUMAN; MARK4S; MARKL 1; MARKL1; Nbla00650; Putative protein product of Nbla00650
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-752
  • 氨基酸序列
    MSSRTVLAPG NDRNSDTHGT LGSGRSSDKG PSWSSRSLGA RCRNSIASCP EEQPHVGNYR LLRTIGKGNF AKVKLARHIL TGREVAIKII DKTQLNPSSL QKLFREVRIM KGLNHPNIVK LFEVIETEKT LYLVMEYASA GEVFDYLVSH GRMKEKEARA KFRQIVSAVH YCHQKNIVHR DLKAENLLLD AEANIKIADF GFSNEFTLGS KLDTFCGSPP YAAPELFQGK KYDGPEVDIW SLGVILYTLV SGSLPFDGHN LKELRERVLR GKYRVPFYMS TDCESILRRF LVLNPAKRCT LEQIMKDKWI NIGYEGEELK PYTEPEEDFG DTKRIEVMVG MGYTREEIKE SLTSQKYNEV TATYLLLGRK TEEGGDRGAP GLALARVRAP SDTTNGTSSS KGTSHSKGQR SSSSTYHRQR RHSDFCGPSP APLHPKRSPT STGEAELKEE RLPGRKASCS TAGSGSRGLP PSSPMVSSAH NPNKAEIPER RKDSTSTPNN LPPSMMTRRN TYVCTERPGA ERPSLLPNGK ENSSGTPRVP PASPSSHSLA PPSGERSRLA RGSTIRSTFH GGQVRDRRAG GGGGGGVQNG PPASPTLAHE AAPLPAGRPR PTTNLFTKLT SKLTRRVADE PERIGGPEVT SCHLPWDQTE TAPRLLRFPW SVKLTSSRPP EALMAALRQA TAAARCRCRQ PQPFLLACLH GGAGGPEPLS HFEVEVCQLP RPGLRGVLFR RVAGTALAFR TLVTRISNDL EL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Serine/threonine-protein kinase. Phosphorylates the microtubule-associated protein MAPT/TAU. Also phosphorylates the microtubule-associated proteins MAP2 and MAP4. Involved in regulation of the microtubule network, causing reorganization of microtubules into bundles. Required for the initiation of axoneme extension during cilium assembly. Regulates the centrosomal location of ODF2 and phosphorylates ODF2 in vitro. Plays a role in cell cycle progression, specifically in the G1/S checkpoint. Reduces neuronal cell survival. Plays a role in energy homeostasis by regulating satiety and metabolic rate. Promotes adipogenesis by activating JNK1 and inhibiting the p38MAPK pathway, and triggers apoptosis by activating the JNK1 pathway. Phosphorylates mTORC1 complex member RPTOR and acts as a negative regulator of the mTORC1 complex, probably due to disruption of the interaction between phosphorylated RPTOR and the RRAGA/RRAGC heterodimer which is required for mTORC1 activation.
  • 基因功能参考文献：
    1. data demonstrate that miR-515-5p dramatically inhibits cell migration by directly down-regulating MARK4 expression in two different cancer types PMID: 26882547
    2. Our results show that MARK4 acts as a negative regulator of the Hippo kinase cassette to promote YAP/TAZ activity and that loss of MARK4 restrains the tumorigenic properties of breast cancer cells. PMID: 28183853
    3. Atypical PKC phosphorylates serine-threonine residues of MARK4. PMID: 26346160
    4. Suggest role for polypyrimidine-tract binding protein in regulating alternative splicing of MARK4 in gliomas. PMID: 25578778
    5. MARK4 is a key component in the regulation of microtubule dynamics and has major role in cell cycle progression, particularly at the G1/S transition. PMID: 25123532
    6. Molecular dynamic simulation data predicted the three dimensional structure for the kinase domain of MARK4 and its structural properties. PMID: 24763618
    7. A strong and significant elevation of MARK4 expression and MARK4-tau interactions in AD brains correlates with the Braak stages of the disease, suggesting that the MARK4-tau interactions are of functional importance in the progression of AD. PMID: 23666762
    8. MARK4 is a critical positive regulator of early steps in ciliogenesis. PMID: 23400999
    9. MARK4 is a new negative regulator of mTORC1 PMID: 23184942
    10. The balance between the MARK4 isoforms is carefully guarded during neural differentiation but may be subverted in gliomagenesis. PMID: 22156016
    11. Even though an association of the polymorphisms rs2850328 and rs2395 and breast cancer was not detected in our case-control study population, other variants within the PPP3CA and MARK4 genes may still be associated with breast cancer. PMID: 22506312
    12. Observational study, meta-analysis, and genome-wide association study of gene-disease association and gene-gene interaction. (HuGE Navigator) PMID: 20460622
    13. demonstrated that the endogenous MARK4L colocalizes with centrosomes at all mitotic stages and resides in centrosome-enriched fractions. PMID: 19759416
    14. Normally expressed in neural progenitors, re-expressed in gliomas and may become a target of amplification upon chr. 19 rearrangement. PMID: 12735302
    15. MARK4 is likely to be directly involved in microtubule organization in neuronal cells PMID: 14594945
    16. NUAK1 and MARK4 are substrates of USP9X PMID: 18254724

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  • 亚细胞定位：
    Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, microtubule organizing center. Cytoplasm, cytoskeleton, cilium basal body. Cytoplasm, cytoskeleton, cilium axoneme. Cytoplasm. Cell projection, dendrite.
  • 蛋白家族：
    Protein kinase superfamily, CAMK Ser/Thr protein kinase family, SNF1 subfamily
  • 组织特异性：
    Ubiquitous. Isoform 2 is brain-specific. Expressed at highest levels in brain and testis. Also expressed in heart, lung, liver, muscle, kidney and spleen.
  • 数据库链接：

    HGNC: 13538

    OMIM: 606495

    KEGG: hsa:57787

    STRING: 9606.ENSP00000262891

    UniGene: Hs.34314