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  4. Recombinant Human Methyl-CpG-binding domain protein 1 (MBD1)

Recombinant Human Methyl-CpG-binding domain protein 1 (MBD1)

  • 货号：
    CSB-YP883444HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP883444HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP883444HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP883444HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP883444HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    MBD1
  • Uniprot No.：
  • 别名：
    CXXC 3; CXXC-type zinc finger protein 3; CXXC3; MBD 1; Mbd1; MBD1_HUMAN; MECP1 COMPLEX; Methyl CpG binding domain protein 1; Methyl CpG binding domain protein 1 isoform PCM1; Methyl CpG binding protein MBD1; Methyl CpG binding protein splice variant 1; Methyl CpG binding protein splice variant 2; Methyl CpG binding protein splice variant 3 ; Methyl CpG binding protein splice variant 4; Methyl-CpG-binding domain protein 1; Methyl-CpG-binding protein MBD1; PCM 1; PCM1; Protein containing methyl-CpG-binding domain 1; Regulator of fibroblast growth factor 2 (FGF 2) transcription; RFT; The regulator of fibroblast growth factor 2(FGF 2) transcription
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-605
  • 氨基酸序列
    MAEDWLDCPA LGPGWKRREV FRKSGATCGR SDTYYQSPTG DRIRSKVELT RYLGPACDLT LFDFKQGILC YPAPKAHPVA VASKKRKKPS RPAKTRKRQV GPQSGEVRKE APRDETKADT DTAPASFPAP GCCENCGISF SGDGTQRQRL KTLCKDCRAQ RIAFNREQRM FKRVGCGECA ACQVTEDCGA CSTCLLQLPH DVASGLFCKC ERRRCLRIVE RSRGCGVCRG CQTQEDCGHC PICLRPPRPG LRRQWKCVQR RCLRGKHARR KGGCDSKMAA RRRPGAQPLP PPPPSQSPEP TEPHPRALAP SPPAEFIYYC VDEDELQPYT NRRQNRKCGA CAACLRRMDC GRCDFCCDKP KFGGSNQKRQ KCRWRQCLQF AMKRLLPSVW SESEDGAGSP PPYRRRKRPS SARRHHLGPT LKPTLATRTA QPDHTQAPTK QEAGGGFVLP PPGTDLVFLR EGASSPVQVP GPVAASTEAL LQEAQCSGLS WVVALPQVKQ EKADTQDEWT PGTAVLTSPV LVPGCPSKAV DPGLPSVKQE PPDPEEDKEE NKDDSASKLA PEEEAGGAGT PVITEIFSLG GTRFRDTAVW LPRSKDLKKP GARKQ
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). Acts as transcriptional repressor and plays a role in gene silencing by recruiting ATF7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Probably forms a complex with SETDB1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Isoform 1 and isoform 2 can also repress transcription from unmethylated promoters.
  • 基因功能参考文献：
    1. MBD1 may be a tumor suppressor gene in advanced colorectal cancer (CRC)and affect the development and metastasis of CRC by regulating 8 tumor suppressor genes through binding with SP1. PMID: 28473981
    2. Our findings imply that reduced stability and enhanced dynamics of MBD1 or MBD6 is the origin of ATP7B dysfunction in Wilson disease patients with the G85V or G591D mutation. PMID: 27744583
    3. MBD1 regulates localization and activity of Tet1 in a CXXC3 domain-dependent manner. PMID: 28449087
    4. the binding of MBD1 to nucleosomes demonstrates sequence preferences depending on the position of the methyl groups on the nucleosome. PMID: 28377300
    5. c-myc plays a key role in MBD1 mediated epigenetic silencing of KEAP1. PMID: 26980696
    6. Molecular dynamic simulation reveals mechanism of the recognition of dimethylated CpG sites by MBD1 protein. PMID: 25658035
    7. MBD1 targets short interspersed nuclear elements, but does not exclude RNA Polymerase III. PMID: 25798578
    8. This study investigates the genetic association between methyl-CpG-binding domain (MBD) gene polymorphisms and schizophrenia. PMID: 24849540
    9. We demonstrated the importance of Aire's interaction with the ATF7ip-MBD1 protein complex in maintaining central tolerance PMID: 24464130
    10. an important function of MBD1 in DNA repair and mediation of chemoradioresistance of cancer cells PMID: 23588667
    11. higher MBD1 expression correlated with lymph node metastasis and poor survival in pancreatic cancer; gain- and loss-of-function studies in vitro validated MBD1 as a potent oncogene promoting pancreatic cancer cell invasion and epithelial-mesenchymal transition PMID: 23331011
    12. MBD1-containing chromatin associated factor 2, epithelial malignancy-related vimentin and exocytosis-related annexin A2 were changed upon exposure to airborne nanoparticle PM(0.056). PMID: 21491466
    13. This review tries to match MeCP2 structural domains, or their lack, and specific chromatin features needed for proper recruitment of MeCP2 to its functions as either activator or repressor. We specifically focused on MeCP2's role in Rett syndrome PMID: 21326358
    14. lack of binding to mCpG and interaction with NuRD/Mi2 components HDAC1 and MTA2 PMID: 12124384
    15. investigation of role of specific mutations in autism PMID: 12384770
    16. Results show that methyl-CpG binding domain protein 1 (MBD1) is expressed in tumor cells, but methyl-CpG binding domain protein 2 (MBD2) and methyl CpG binding protein 2 (MeCP2) are not. PMID: 12646234
    17. MCAF interacts with the transcriptional repression domain of MBD1 PMID: 12665582
    18. Suv39h1 enhanced MBD1-mediated transcriptional repression via MBD, not the C-terminal transcriptional repression domain of MBD1. MBD1 links to histone deacetylases through Suv39h1, causing methylation and deacetylation of histones for gene inactivation PMID: 12711603
    19. Additional evidence of variable expression in the Rett disorder phenotype is presented by a small mixed gender group of children with autistic disorder. PMID: 12770674
    20. role in chromatin compaction PMID: 12788925
    21. determination of link of p59 OASL with MBD1 transcriptional control in the context of an interferon-stimulated cell, and provision of basis for future studies to examine the functional role of this interaction PMID: 14728690
    22. regulates G1-S transition and apoptosis via p53/p21(Waf1) pathway PMID: 15081425
    23. Data show that the methyl-CpG binding protein MBD1 forms a stable complex with histone H3-K9 methylase SETDB1. PMID: 15327775
    24. These data suggest that MBD1.MCAF1.SETDB1 complex facilitates the formation of heterochromatic domains, emphasizing the role of MCAF/AM family proteins in epigenetic control, and describe a new family member, MCAF2. PMID: 15691849
    25. Methyl-CpG binding protein MBD1 binds to teneurin 1. PMID: 15777793
    26. PML-RARalpha functions by recruiting an HDAC3-MBD1 complex that contributes to the establishment and maintenance of the silenced chromatin state PMID: 16432238
    27. MBD1- and MCAF1-mediated heterochromatin formation involves SUMO modification PMID: 16757475
    28. Sex-specific time windows for concomitant upregulation of MBD1 are associated with prenatal remethylation of the human male and female germ line. PMID: 16998846
    29. Transcriptional silencing by MBD1 is regulated by a PIAS-mediated conjugation of SUMO1, which antagonizes the formation of a repressive complex with SETDB1. PMID: 17066076
    30. MBD1 and Polycomb group proteins have overlapping roles in epigenetic gene silencing and heterochromatin foci formation through their interactions. PMID: 17428788
    31. some insight into the functional mechanism of MBD1 in the development of pancreatic cancer PMID: 18445260
    32. Silencing of MBD1 and MeCP2 in prostate-cancer-derived PC3 cells produces differential gene expression profiles and cellular phenotypes PMID: 18666890
    33. Results suggest that MBD1 polymorphisms might be involved in the development of lung cancer in Chinese population. PMID: 18668384

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  • 亚细胞定位：
    Nucleus. Nucleus matrix. Nucleus speckle. Chromosome.
  • 组织特异性：
    Widely expressed.
  • 数据库链接：

    HGNC: 6916

    OMIM: 156535

    KEGG: hsa:4152

    STRING: 9606.ENSP00000405268

    UniGene: Hs.405610