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  4. Recombinant Human Methyl-CpG-binding domain protein 2 (MBD2), partial

Recombinant Human Methyl-CpG-binding domain protein 2 (MBD2), partial

  • 中文名称：
    人MBD2重组蛋白
  • 货号：
    CSB-YP890654HU1
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人MBD2重组蛋白
  • 货号：
    CSB-EP890654HU1-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人MBD2重组蛋白
  • 货号：
    CSB-BP890654HU1
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人MBD2重组蛋白
  • 货号：
    CSB-MP890654HU1
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    MBD2
  • Uniprot No.：
  • 别名：
    Demethylase; DMTase; MBD 2; Mbd2; MBD2_HUMAN; MBD2a; Methyl CpG binding domain protein 2; Methyl CpG binding protein MBD2; Methyl-CpG-binding domain protein 2; Methyl-CpG-binding protein MBD2; NY CO 41
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemimethylated DNA as well. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing. Functions as a scaffold protein, targeting GATAD2A and GATAD2B to chromatin to promote repression. May enhance the activation of some unmethylated cAMP-responsive promoters.
  • 基因功能参考文献：
    1. Suggest that reduced MBD2 expression could contribute to chronic airway inflammation in COPD. PMID: 29535511
    2. knockdown of MBD2 by siRNA attenuated vancomycin-induced apoptosis, caspase activity, and the expression of BAX and cleaved caspase 3. PMID: 29022913
    3. CpG and methylation-dependent DNA binding and dynamics of the MBD2 protein at the single-molecule level have been reported. PMID: 28637186
    4. Results show that MBD2 mediates epigenetic silencing of Htra1. PMID: 27388476
    5. MBD2 has a critical role in 'rewriting' the cancer methylome at specific regulatory regions. PMID: 27593931
    6. MBD2 expression was elevated in HCC tissue, which suggesting MBD2 as a candidate prognostic marker of HCC. PMID: 27315121
    7. downregulation of miR-221 inhibits cell migration and invasion at least partially through targeting MBD2 in the human OSCC cell line UM1. PMID: 26788506
    8. Specifically, methyl-CpG-binding domain protein 2 (MBD2) is revealed to be recruited to DNA damage sites after laser microirradiation, which was mediated through MBD domain and MBD2 C-terminus. PMID: 26827827
    9. MBD2 targets short interspersed nuclear elements, but does not exclude RNA Polymerase III. PMID: 25798578
    10. The dynamics of MBD2 deposition across methylated DNA regions was associated with the oncogenic transformation of human mammary cells. PMID: 26007656
    11. Data suggest that MBD2 binds primarily at highly methylated regions, with a strong preference for CpG islands and highlights that MBD2 binding sites display increased methylation in primary breast cancer tissues as compared to normal mammary cells. PMID: 24927503
    12. Biophysical analyses show that the MBD2IDR is an intrinsically disordered region (IDR). However, despite this inherent disorder, MBD2IDR increases the overall binding affinity of MBD2 for methylated DNA. PMID: 25753662
    13. This study investigates the genetic association between methyl-CpG-binding domain (MBD) gene polymorphisms and schizophrenia. PMID: 24849540
    14. alternatively spliced isoforms support pluripotency of stem cells PMID: 24813856
    15. The methylated-DNA binding protein MBD2 enhances NGFI-A (egr-1)-mediated transcriptional activation of the glucocorticoid receptor. PMID: 25135974
    16. These data point to a potential new approach in targeting the DNA methylation machinery by combination of MBD2 and DNMT inhibitors. PMID: 25178277
    17. MBD2 seems to play a selective role in gene repression depending on the CpG content of the promoter region PMID: 23888954
    18. Methylated DNA binding domain protein 2 (MBD2) coordinately silences gene expression through activation of the microRNA hsa-mir-496 promoter in breast cancer cell line. PMID: 24204564
    19. reduced mRNA expression of MBD2 and MBD3 is implicated in gastric carcinogenesis. PMID: 24338710
    20. global DNA hypomethylation is one of the possible epigenetic variations associated with the complex etiology of TOF and significantly correlates with the aberrant expression of MBD2 mRNA in patients with TOF. PMID: 23820632
    21. MBD2 binds a significant fraction of the hypomethylated genes. PMID: 23955541
    22. Abnormal expression levels of DNA (cytosine-5-)-methyltransferase 1 and MBD2 mRNA may be important causes of the global hypomethylation observed in CD4+T cells in systemic lupus erythematosus. PMID: 23127209
    23. In metastatic colorectal cancer cells, reduced levels of miR-221* and miR-224 increase levels of MBD2, thereby decreasing expression of the metastasis suppressor maspin. PMID: 23770133
    24. MBD3 is enriched at active promoters, whereas MBD2 is bound at methylated promoters and enriched at exon sequences of active genes. PMID: 23361464
    25. These factors lead to a binding affinity hierarchy of p66alpha for the different MBD2 homologues (MBD2 approximately MBD3 > MBD3L1 approximately MBD3L2). PMID: 23239876
    26. repressive functions of MBD2-containing NuRD complexes are dependent on cooperative interactions between the major domains of CHD4 with histones and DNA and on binding of methylated DNA by MBD2 PMID: 23071088
    27. MiR-373 behaves as a direct transcriptional target and negative regulator of MBD2 activity through a feedback loop of CpG island methylation in hilar cholangiocarcinoma PMID: 22876037
    28. The association between MBD2 binding and transcriptional repression weakened as the distance between binding site and TSS increased, suggesting that MBD2 represses transcriptional initiation PMID: 22048253
    29. A two-stage association study identifies methyl-CpG-binding domain protein 2 gene polymorphisms as candidates for breast cancer susceptibility. PMID: 22258532
    30. These results show a role for MBD2 in cancer progression and provide support for the prospect of targeting MBD2 therapeutically in aggressive breast cancers. PMID: 21693597
    31. MBD2 overexpression during gliomagenesis may drive tumor growth by suppressing the antiangiogenic activity of a key tumor BAI1. PMID: 21724586
    32. human epsilon-globin gene is subject to multilayered silencing mediated in part by MBD2 PMID: 21296012
    33. In hilar cholangiocarcinoma, down-expression of miR-373 leads to increase of MBD2, which in turn suppresses the methylation-mediated gene such as RASSF1A. PMID: 21086164
    34. This study demonstrated that patients with SLE had a significantly lower level of DNA methylation than the controls, and that expression of both DNMT1 and MBD2 mRNA was significantly increased in the SLE patients compared with controls. PMID: 21078759
    35. Expression level of MBD2 is significantly lower in endometriotic lesions compared with disease-free controls. PMID: 21316665
    36. miR-373 is a methylation-mediated gene and the implication of MBD2 in methylation-mediated suppression of miR-373 plays an important role in tumourigenesis and development in hilar cholangiocarcinoma. PMID: 21165562
    37. The anti-TNFalpha biological agents do not seem to affect DNA methylation and mRNA expressions of DNMT1 and MBD2 in RA PMID: 20937307
    38. MBD2 and ERalpha drive opposite effects on pS2 expression, which are associated with specific steady state levels of histone H3 acetylation and methylation marks PMID: 20300195
    39. Methyl-CpG binding domain protein 2 represses transcription from hypermethylated pi-class glutathione S-transferase gene promoters in hepatocellular carcinoma cells PMID: 11960994
    40. MBD2 protein activates CpG sites within the promoter region of reporter genes PMID: 12177048
    41. interaction with two highly related p66 proteins PMID: 12183469
    42. interacts with latency-associated nuclear antigen of Kaposi's Sarcoma-associated herpesvirus (KSHV)to tether KSHV to cell chromosomes PMID: 12388720
    43. MBDin relieves MBD2 repression potential and reactivates transcription from methylated promoters PMID: 12588985
    44. Results show that methyl-CpG binding domain protein 1 (MBD1) is expressed in tumor cells, but methyl-CpG binding domain protein 2 (MBD2) and methyl CpG binding protein 2 (MeCP2) are not. PMID: 12646234
    45. MBD2a and RNA helicase A cooperatively enhanced CREB-dependent gene expression. PMID: 12665568
    46. MBD2 has a role in the methylation-mediated inhibition of ribosomal RNA gene expression PMID: 14610093
    47. Antisenses oligoDNA suppresses tumor growth in nude mice, a potential anticaner therapy approach. PMID: 14688029
    48. MBD2 gene expression may be significant factor in tumorigenesis. PMID: 15112265
    49. role of MBD3L1 as a methylation-dependent transcriptional repressor that may interchange with MBD3 as an MBD2-interacting component of the NuRD complex PMID: 15456747
    50. MBD3L2 interacts with MBD3 and components of the NuRD complex and can oppose MBD2-MeCP1-mediated methylation silencing PMID: 15701600

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  • 亚细胞定位：
    Nucleus. Note=Nuclear, in discrete foci. Detected at replication foci in late S phase.
  • 组织特异性：
    Highly expressed in brain, heart, kidney, stomach, testis and placenta.
  • 数据库链接：

    HGNC: 6917

    OMIM: 603547

    KEGG: hsa:8932

    STRING: 9606.ENSP00000256429

    UniGene: Hs.25674