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  4. Recombinant Human Methyl-CpG-binding domain protein 3 (MBD3)

Recombinant Human Methyl-CpG-binding domain protein 3 (MBD3)

  • 货号：
    CSB-YP013532HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP013532HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP013532HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP013532HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP013532HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    MBD3
  • Uniprot No.：
  • 别名：
    AI181826; AU019209; MBD 3; Mbd3; MBD3: methyl CpG binding domain protein 3; MBD3_HUMAN; Methyl CpG binding domain protein 3; Methyl CpG binding protein MBD3; Methyl-CpG-binding domain protein 3; Methyl-CpG-binding protein MBD3
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-291
  • 氨基酸序列
    MERKRWECPA LPQGWEREEV PRRSGLSAGH RDVFYYSPSG KKFRSKPQLA RYLGGSMDLS TFDFRTGKML MSKMNKSRQR VRYDSSNQVK GKPDLNTALP VRQTASIFKQ PVTKITNHPS NKVKSDPQKA VDQPRQLFWE KKLSGLNAFD IAEELVKTMD LPKGLQGVGP GCTDETLLSA IASALHTSTM PITGQLSAAV EKNPGVWLNT TQPLCKAFMV TDEDIRKQEE LVQQVRKRLE EALMADMLAH VEELARDGEA PLDKACAEDD DEEDEEEEEE EPDPDPEMEH V
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Acts as transcriptional repressor and plays a role in gene silencing. Does not bind to DNA by itself. Binds to DNA with a preference for sites containing methylated CpG dinucleotides (in vitro). Binds to a lesser degree DNA containing unmethylated CpG dinucleotides. Recruits histone deacetylases and DNA methyltransferases.
  • 基因功能参考文献：
    1. Study have demonstrated that MBD3/ NuRD inhibits formation of liver induced cancer stem cells (iCSCs). In addition, MBD3 suppression induces c-JUN, resulting in the induction of pluripotent genes in iCSCs. PMID: 27894081
    2. Suggest that MBD3 exerts influences on both active and silenced genes in glioma cells. PMID: 27835581
    3. MBD3 inhibits epithelial-mesenchymal transition in pancreatic cancer cells probably via TGF-beta/Smad signaling and may be a candidate for diagnostics and prognosis of pancreatic cancer. PMID: 27898661
    4. Overexpression of MBD3 is associated with neoplasms. PMID: 27465550
    5. Insufficient MBD3 induced by small interfering RNA (siRNA) was found to result in a global DNA hypermethylation as well as increased methylation in the promoter CpG islands (CGIs) of a number of cell cycle related genes. PMID: 25753672
    6. This study investigates the genetic association between methyl-CpG-binding domain (MBD) gene polymorphisms and schizophrenia. PMID: 24849540
    7. These data suggest that MBD3, and by extension the NuRD complex, may have multiple roles in fine tuning expression for both active and silent genes, representing an important step in defining regulatory mechanisms PMID: 24385926
    8. reduced mRNA expression of MBD2 and MBD3 is implicated in gastric carcinogenesis. PMID: 24338710
    9. MBD3 is enriched at active promoters, whereas MBD2 is bound at methylated promoters and enriched at exon sequences of active genes. PMID: 23361464
    10. These factors lead to a binding affinity hierarchy of p66alpha for the different MBD2 homologues (MBD2 approximately MBD3 > MBD3L1 approximately MBD3L2). PMID: 23239876
    11. MBD3 mutations are not responsible for ICR1 hypomethylation in Silver-Russell syndrome. PMID: 20004753
    12. MBD3 is highly expressed in glioblastome multiforme compared to astrocytoma and anaplastic astrocytoma PMID: 11836615
    13. interaction with two highly related p66 proteins PMID: 12183469
    14. localization in Aurora-A-positive centrosomes in M phase PMID: 12354758
    15. MBD3L2 interacts with MBD3 and components of the NuRD complex and can oppose MBD2-MeCP1-mediated methylation silencing PMID: 15701600
    16. MBD3 assembles into mutually exclusive distinct Mi-2/NuRD-like complex, called MBD3/NuRD. PMID: 16428440
    17. This is the first demonstration that MBD3 is involved in inducing and maintaining the demethylated state of a specific promoter. PMID: 17452452
    18. data demonstrate a causal relationship between MBD3 and DNA demethylation of genomic targets in cells. PMID: 18602768
    19. Study demonstrates a direct role of the MBD3, a subunit of nucleosome remodeling and deacetylase corepressor complex complex in aberrant gene repression and transmission of epigenetic repressive marks in acute promyelocytic leukemia. PMID: 18644863
    20. These findings collectively support a role for MBD3 in cell cycle progression and cell death as a modulator of HDAC-mediated transcription. PMID: 19041848

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  • 亚细胞定位：
    Nucleus. Chromosome. Note=Nuclear, in discrete foci. Detected on chromatin, at promoter regions of active genes.
  • 数据库链接：

    HGNC: 6918

    OMIM: 603573

    KEGG: hsa:53615

    STRING: 9606.ENSP00000156825

    UniGene: Hs.178728