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  4. Recombinant Human Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A)

Recombinant Human Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A)

  • 货号：
    CSB-YP880971HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP880971HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP880971HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP880971HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP880971HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    MAP1LC3A
  • Uniprot No.：
  • 别名：
    ATG8E; Autophagy related protein LC3 A; Autophagy related ubiquitin like modifier LC3 A; Autophagy-related protein LC3 A; Autophagy-related ubiquitin-like modifier LC3 A; LC3; LC3A; MAP1 light chain 3 like protein 1; MAP1 light chain 3-like protein 1; MAP1A/1B light chain 3 A; MAP1A/MAP1B LC3 A; MAP1A/MAP1B light chain 3 A; MAP1ALC3; MAP1BLC3; Map1lc3a; Microtubule associated proteins 1A/1B light chain 3; Microtubule-associated protein 1 light chain 3 alpha; Microtubule-associated proteins 1A and 1B, light chain 3; Microtubule-associated proteins 1A/1B light chain 3A; MLP3A_HUMAN
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-120
  • 氨基酸序列
    MPSDRPFKQR RSFADRCKEV QQIRDQHPSK IPVIIERYKG EKQLPVLDKT KFLVPDHVNM SELVKIIRRR LQLNPTQAFF LLVNQHSMVS VSTPIADIYE QEKDEDGFLY MVYASQETFG
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). While LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover.
  • 基因功能参考文献：
    1. Expression and Clinical Significance of LC-3 and P62 in Non-small Cell Lung Cancer PMID: 29945702
    2. High LC3 Expression is Associated with Cancer Progression and Chemo-Resistance in Esophageal Squamous Cell Carcinoma. PMID: 29022200
    3. Data suggest that, in patients with diabetic kidney disease, urinary excretion of mRNAs for MAP1LC3A, WIPI2, and RB1CC1 is down-regulated as compared to healthy control subjects; these transcripts may serve as urinary autophagy biomarkers. (MAP1LC3A = microtubule associated protein 1 light chain 3; WIPI2 = WD repeat domain phosphoinositide-interacting protein 2; RB1CC1 = RB1 inducible coiled-coil 1) PMID: 28760651
    4. Autophagy proteins were discovered on phagosomes engulfing foreign bacteria, leading to the proposal of LC3-associated phagocytosis (LAP). [review] PMID: 28755428
    5. data raise important issues regarding the interpretation of LC3 lipidation data and the use of autophagy modulators PMID: 28296541
    6. High LC3 expression is associated with endometriosis. PMID: 28348069
    7. Co-expression of FKBP8 with LC3A profoundly induces Parkin-independent mitophagy. Strikingly, even when acting as a mitophagy receptor, FKBP8 avoids degradation by escaping from mitochondria. In summary, this study identifies novel roles for FKBP8 and LC3A, which act together to induce mitophagy. PMID: 28381481
    8. we report that autophagy is associated with apoptosis processes, involving LC3 and TRIF-colocation in human HCC cells. Regulation of autophagy and the TLR3-TRIF pathway may be effective in the treatment of liver cancer. PMID: 27338037
    9. The autophagy induced by 2-PCPA requires LC3-II processing machinery..2-PCPA treatment induces the change of global gene expression program, including a series of autophagy-related genes, such as SQSTM1/p62. Taken together, our data indicate that KDM1A/LSD1 inhibitors induce autophagy through affecting the expression of autophagy-related genes and in a BECN1-independent manner PMID: 28800922
    10. LC3 and p62/SQSTM1 have roles in early-stage non-small cell lung cancer PMID: 27250032
    11. Hotspot mutations in the arginine-rich stretch in MAP1LC3A resulting in reduced cleavage of MAP1LC3A by ATG4B both in vitro and in vivo, suggesting a functional implication of this gene mutation in cancer development. PMID: 27256984
    12. analysis of soluble SQSTM1 complexes and soluble complexes formed between SQSTM1 oligomers and LC3 using a combination of fluorescence microscopy-based biophysical approaches in living cells PMID: 27442348
    13. we found that ATG14 interacted with Ulk1 and LC3, and knock down of Ulk1 prevented the lipidation of LC3 and autophagy in HeLa-ATG14 cells. We also identified a phosphatidylethanolamine (PE) binding region in ATG14, and the addition of Ulk1 to Hela-ATG14 cells decreased the ATG14-PE interaction. PMID: 28069524
    14. Legionella pneumophila RavZ extracts LC3-PE from the membrane before deconjugation. RavZ initially recognizes the LC3 molecule on membranes via its N-terminal LC3-interacting region (LIR) motif. PMID: 28395732
    15. The results are consistent with a model in which RPIA suppresses autophagy and LC3 processing by modulation of redox signaling. PMID: 27328773
    16. can act as a negative mediator in autophagy through stimulation of the AKT-MTORC1 pathway and direct interaction with LC3. PMID: 27540684
    17. Data show that p62 interaction with LC3, leading to the delivery of p62 and its cargoes to the autophagosome. PMID: 28740232
    18. LC3 puncta were still present in the granular layer of nonlesional psoriatic epidermis but at a much lower level compared with healthy adult epidermis, whereas in lesional psoriatic skin, LC3 was not expressed at all. PMID: 27021405
    19. Results suggest that P. gingivalis internalization is not necessary for LC3 lipidation. PMID: 26452236
    20. Although neither GABARAPs nor LC3s are required for autophagosome biogenesis, loss of all Atg8s yields smaller autophagosomes and a slowed initial rate of autophagosome formation. PMID: 27864321
    21. Methylation silencing of MAP1LC3Av1 promotes gastric carcinogenesis. PMID: 28214334
    22. Development of LC3/GABARAP sensors containing a LIR and a hydrophobic domain to monitor autophagy. PMID: 28320742
    23. Aldo was revealed to induce autophagy, as indicated by the increased conversion from microtubuleassociated protein 1A/1Blight chain 3 (LC3)I to LC3II, the increased expression levels of autophagyrelated gene 7 (Atg7) and the increased degradation of p62, which was accompanied by MC proliferation PMID: 27748808
    24. LC3 overexpression is associated with colorectal cancer. PMID: 27444698
    25. these findings were reversed with the LC3I/II-shPTEN treatment. Therefore, the loss of PTEN may promote the development of primary resistance to trastuzumab in breast cancer via autophagy defects PMID: 26563373
    26. autophagy of cytoplasmic bulk cargo was completely LC3 independent. PMID: 26237084
    27. Results of our present study demonstrated that beclin 1 and LC3 immunoreactivity increased in carcinoma cells following exemestane treatment and that the status of pre-treatment stromal beclin 1 is associated with higher carcinoma cell proliferation and poor clinical and pathological responses to NAE PMID: 26984766
    28. Consistent with the in vitro results, we observed that the expression of autophagy marker LC3 was increased. PMID: 26676801
    29. Up-regulation of LC3 is associated with carcinogenesis and progression of Salivary Adenoid Cystic Carcinoma. PMID: 26350055
    30. up-regulated expression during the monocyte-to-dendritic cell transition PMID: 26395023
    31. LC3 is exploited by coxsackievirus in both autophagy-dependent and -independent manners in vivo PMID: 26090585
    32. Autophagy status, as determined by combined LC3, Beclin 1 and p62 expression, might be independently associated with poor survival in patients with gastric cancer. PMID: 26722036
    33. Western blot and immunofluorescence validated antibody specificity, knockdown efficiency and autophagy induction prior to fixation in formalin and embedding in paraffin. LC3B and p62 antibodies were validated on cell pellets PMID: 26150155
    34. In colorectal cancer, differentiation degree and lymph node metastasis were associated with LC3 overexpression. LC3 expression was significantly higher in cancerous tissue than in adjacent tissue. PMID: 26097572
    35. high nuclear HMGB1 levels and cytoplasmic HMGB1 expression predicted poor overall survival (OS) and disease-free survival (DFS). Meanwhile, high LC3 expression was associated with favorable prognosis. PMID: 26084608
    36. These results demonstrate the essential role of BECN1 in the functional formation of autophagosomes, but not in LC3B lipidation. PMID: 25955014
    37. The s show that LC3 is recruited specifically around secreting Shigella flexneri bacteria that are still present in vacuoles formed during entry and cell-to-cell spread. PMID: 26015503
    38. Downregulation of beta 3 -integrin with cRGD peptide resulted in enhanced LC3-II and Beclin-1 and decreased Bcl-2 expression. PMID: 25824726
    39. These data add novel mechanistic insights into the regulation of noncanonical LC3 lipidation and its associated processes, including LC3-associated phagocytosis. PMID: 25484071
    40. direct association with LC3 and p62 is promoted in osteoblasts by bioactive silica nanoparticles PMID: 24806912
    41. Results showed that detergent-insoluble LC3-I and -II levels were significantly higher in multiple system atrophy brain tissue than those in controls PMID: 22959883
    42. Clusterin facilitates stress-induced lipidation of LC3 and autophagosome biogenesis to enhance cancer cell survival. PMID: 25503391
    43. This study aimed to explore autophagy-related protein light chain 3 (LC3)-II expression levels in thyroid diseases. PMID: 25661375
    44. CHDH is required for PARK2-mediated mitophagy for the recruitment of SQSTM1 and LC3 onto the mitochondria for cargo recognition PMID: 25483962
    45. PLP2-TM interacts with the key autophagy regulators, LC3 and Beclin1, and promotes Beclin1 interaction with STING, the key regulator for antiviral IFN signaling, to negatively regulate antiviral innate immunity. PMID: 25311841
    46. highlight the role that VAMP3 and VAMP7 play in selection of the pathways leading to generation of ultrastructurally different LC3 compartments PMID: 25046114
    47. Patients with strong immunoreactivity to BECLIN 1 or LC3 had a significantly better overall survival (OS) than patients with negative to moderate immunoreactivity (p = 0.036 and 0.018, respectively). PMID: 25871810
    48. A significant association of LC3 expression with disease progression including a large tumor size, higher stage, and worse prognosis of hepatocellular carcinoma. PMID: 25005847
    49. high level of expression of BECLIN 1 and LC3 in tumours is well correlated with the overall survival of the patients. PMID: 25136588
    50. Results show that acetylation-deacetylation cycle ensures that LC3 effectively redistributes in an activated form from nucleus to cytoplasm, where it plays a central role in autophagy. PMID: 25601754

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  • 亚细胞定位：
    Cytoplasm, cytoskeleton. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor. Cytoplasmic vesicle, autophagosome. Note=LC3-II binds to the autophagic membranes.
  • 蛋白家族：
    ATG8 family
  • 组织特异性：
    Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes.
  • 数据库链接：

    HGNC: 6838

    OMIM: 601242

    KEGG: hsa:84557

    STRING: 9606.ENSP00000363970

    UniGene: Hs.632273