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  4. Recombinant Human Monoglyceride lipase (MGLL)

Recombinant Human Monoglyceride lipase (MGLL)

  • 中文名称：
    人MGLL重组蛋白
  • 货号：
    CSB-EP013787HU
  • 规格：
    ￥1344
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    MGLL
  • Uniprot No.：
  • 别名：
    EC 3.1.1.23; HU K5 ; HU-K5; HUK5 ; Lysophospholipase homolog; Lysophospholipase like; Lysophospholipase-like; MAGL; MGL; MGLL; MGLL_HUMAN; Monoacylglycerol lipase; Monoglyceride lipase
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length
  • 来源：
    E.coli
  • 分子量：
    60.3kDa
  • 表达区域：
    1-303aa
  • 氨基酸序列
    MPEESSPRRTPQSIPYQDLPHLVNADGQYLFCRYWKPTGTPKALIFVSHGAGEHSGRYEELARMLMGLDLLVFAHDHVGHGQSEGERMVVSDFHVFVRDVLQHVDSMQKDYPGLPVFLLGHSMGGAIAILTAAERPGHFAGMVLISPLVLANPESATTFKVLAAKVLNLVLPNLSLGPIDSSVLSRNKTEVDIYNSDPLICRAGLKVCFGIQLLNAVSRVERALPKLTVPFLLLQGSADRLCDSKGAYLLMELAKSQDKTLKIYEGAYHVLHKELPEVTNSVFHEINMWVSQRTATAGTASPP
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal GST-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Converts monoacylglycerides to free fatty acids and glycerol. Hydrolyzes the endocannabinoid 2-arachidonoylglycerol, and thereby contributes to the regulation of endocannabinoid signaling, nociperception and perception of pain. Regulates the levels of fatty acids that serve as signaling molecules and promote cancer cell migration, invasion and tumor growth.
  • 基因功能参考文献：
    1. Using a multimethod approach, the s show that the dynamically relevant Trp-289 and Leu-232 residues serve as communication hubs within an allosteric protein network that controls signal propagation to the active site, and thus, regulates active-inactive interconversion of human MGL. PMID: 29379013
    2. RNA interference, specific pharmacological inhibitor JZL-184 and gene knock-in of MAGL were utilized to investigate the effects of MAGL on hepatocellular carcinoma (HCC) cell proliferation, apoptosis, and invasion. MAGL played important roles in both proliferation and invasion of HCC cells. PMID: 27767105
    3. This review summarizes the basics of monoglyceride metabolism and provides an overview on the therapeutic potential of MGL. [Review] PMID: 28213089
    4. MGLL may have a role in progression of gastrointestinal stromal tumors PMID: 27366945
    5. the upregulation of MAGL in hepatocellular carcinoma cells promoted cell growth and invasiveness abilities, and mediated epithelial-mesenchymal transition PMID: 27884159
    6. we clarify the key role of Phe159 and Ile179, two conserved residues within the lid domain, in regulating substrate specificity in MAGL. We conclude by proposing that other structurally related lipases may share this lid-domain-mediated mechanism for substrate specificity. PMID: 28088576
    7. the presence and differential distribution of fatty acid amide hydrolase (FAAH) and monoglyceride lipase (MGLL) in relation to CB1 during the maturation of human oocytes, was investigated. PMID: 26948343
    8. there was evidence that MGLL rs604300 genotype interacts with early life adversity to predict threat-related basolateral amygdala habituation, a neural phenotype linked to the endocannabinoid system and addiction PMID: 26595473
    9. This study unravels a novel mechanism of SND1 function and identifies MGLL as a unique tumor suppressor for HCC. MGLL might function as a homeostatic regulator of Akt restraining its activation. PMID: 26997225
    10. The data highlight specific inter-residue interactions within hMGL PMID: 26555264
    11. Monoacylglycerol lipase sulfenylation might act as an intrinsic neuroprotective mechanism by potentiating 2-AG signaling at CB1 receptors. PMID: 26000748
    12. Molecular dynamics and nudged elastic band simulations were used to explore the conformational transition pathway of the helix alpha4 of human monoacylglycerol lipase. PMID: 25078047
    13. role of monoacylglycerol lipase (MAGL) in the cancer progress PMID: 24633487
    14. Our findings establish that MAGL promotes metastases in nasopharyngeal carcinoma PMID: 25120746
    15. In subcutaneous adipose tissue, DAGL-a mRNA was upregulated and fatty acid amide hydrolase (FAAH) and monoacylglycerol lipase (MAGL) mRNAs were down-regulated in obese subjects, but the diets had no influence. PMID: 24616451
    16. In obese humans, FAAH or MGL activity in adipocytes is not affected by diabetes, dyslipidaemia or other markers of metabolic dysfunction. PMID: 24593280
    17. Mutation of Cys242 was also found to impair inhibition of monoacylglycerol lipase. PMID: 24368842
    18. MGL interaction with a phospholipid membrane bilayer induces regional changes in the enzyme's conformation that favor its recruiting lipophilic substrate from membrane stores to the active site resulting in enhanced MGL catalytic activity. PMID: 23553709
    19. Data indicate that N,N-dimethyl-5-(4-phenoxyphenyl)-2H-tetrazole-2-carboxamide inhibited monoacylglycerol lipase (MAGL) with IC50 0.028 muM. PMID: 23455058
    20. MGL expression declines after peaking in infancy. PMID: 22827915
    21. results suggest that MGL plays a negative regulatory role in phosphatidylinositol-3 kinase/Akt signaling and tumor cell growth PMID: 22349814
    22. No association was found between our inflammatory bowel disease cohort and the candidate single nucleotide polymorphisms for MGL (CD/HC: P=0.37 and UC/HC: P=0.25). PMID: 22664939
    23. MAGL is elevated in androgen-independent versus androgen-dependent human prostate cancer cell lines, and pharmacological or RNA-interference disruption of this enzyme impairs prostate cancer aggressiveness. PMID: 21802006
    24. High MAGL is associated with colorectal cancer. PMID: 21543155
    25. Data present a high-resolution structure of a ligand-bound, soluble form of human monoglyceride lipase. PMID: 21308848
    26. One interval in the FAAH promoter and three intervals in the MGLL gene were associated with high BMI. PMID: 21118518
    27. Arachidonoylglycerol (2-AG) is the most abundant endocannabinoid in the brain and is believed to be hydrolyzed primarily by serine hydrolase monoacylglycerol lipase (MAGL) in a transgenic mouse model with targeted disruption of MAGL. PMID: 20855465
    28. Identification of an active site hydrogen bond network in human monoacylglycerol lipase. PMID: 20464001
    29. An apolar helix covering the active site also gives structural insight into the amphitropic character of MAGL, and likely explains how MAGL interacts with membranes to recruit its substrate. PMID: 19957260
    30. MGL shares the classic fold of the alpha/beta hydrolase family but depicts an unusually large hydrophobic occluded tunnel with a highly flexible lid at its entry and the catalytic triad buried at its end. PMID: 19962385
    31. Study shows that monoacylglycerol lipase (MAGL) is highly expressed in aggressive cancer cells and primary tumors; it regulates a fatty acid network enriched in oncogenic signaling lipids that promotes migration, invasion, survival, and tumor growth. PMID: 20079333
    32. not associated with susceptibility to alcoholism in a Japanese population. PMID: 17621164
    33. full proteomic characterization of hMGL was carried out, which showed (1) an absence of intramolecular disulfide bridges in the functional, recombinant enzyme and (2) the post-translational removal of the enzyme's N-terminal methionine PMID: 18452279
    34. Identification of amino acids critical to the catalytic activity PMID: 18721756
    35. Gene Ontology analysis indicated a significant alteration of oxygen transport (increased hemoglobin gene expression) and lipid metabolism [including monoglyceride lipase and low density lipoprotein receptor-related protein 5 (LRP5) gene]. PMID: 19233690

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  • 亚细胞定位：
    Cytoplasm, cytosol. Membrane; Peripheral membrane protein.
  • 蛋白家族：
    AB hydrolase superfamily, Monoacylglycerol lipase family
  • 组织特异性：
    Detected in adipose tissue, lung, liver, kidney, brain and heart.
  • 数据库链接：

    HGNC: 17038

    OMIM: 609699

    KEGG: hsa:11343

    STRING: 9606.ENSP00000265052

    UniGene: Hs.277035