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  4. Recombinant Human NF-kappa-B-repressing factor (NKRF), partial

Recombinant Human NF-kappa-B-repressing factor (NKRF), partial

  • 中文名称：
    人NKRF重组蛋白
  • 货号：
    CSB-EP015836HU
  • 规格：
    ￥1344
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    NKRF
  • Uniprot No.：
  • 别名：
    NKRF; ITBA4; NRF; NF-kappa-B-repressing factor; NFkB-repressing factor; Protein ITBA4; Transcription factor NRF
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 来源：
    E.coli
  • 分子量：
    81.1kDa
  • 表达区域：
    4-688aa
  • 氨基酸序列
    ILQMAEGIDIGEMPSYDLVLSKPSKGQKRHLSTCDGQNPPKKQAGSKFHARPRFEPVHFVASSSKDERQEDPYGPQTKEVNEQTHFASMPRDIYQDYTQDSFSIQDGNSQYCDSSGFILTKDQPVTANMYFDSGNPAPSTTSQQANSQSTPEPSPSQTFPESVVAEKQYFIEKLTATIWKNLSNPEMTSGSDKINYTYMLTRCIQACKTNPEYIYAPLKEIPPADIPKNKKLLTDGYACEVRCQNIYLTTGYAGSKNGSRDRATELAVKLLQKRIEVRVVRRKFKHTFGEDLVVCQIGMSSYEFPPALKPPEDLVVLGKDASGQPIFNASAKHWTNFVITENANDAIGILNNSASFNKMSIEYKYEMMPNRTWRCRVFLQDHCLAEGYGTKKTSKHAAADEALKILQKTQPTYPSVKSSQCHTGSSPRGSGKKKDIKDLVVYENSSNPVCTLNDTAQFNRMTVEYVYERMTGLRWKCKVILESEVIAEAVGVKKTVKYEAAGEAVKTLKKTQPTVINNLKKGAVEDVISRNEIQGRSAEEAYKQQIKEDNIGNQLLRKMGWTGGGLGKSGEGIREPISVKEQHKREGLGLDVERVNKIAKRDIEQIIRNYARSESHTDLTFSRELTNDERKQIHQIAQKYGLKSKSHGVGHDRYLVVGRKRRKEDLLDQLKQEGQVGHYELVMPQ
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Interacts with a specific negative regulatory element (NRE) 5'-AATTCCTCTGA-3' to mediate transcriptional repression of certain NK-kappa-B responsive genes. Involved in the constitutive silencing of the interferon beta promoter, independently of the virus-induced signals, and in the inhibition of the basal and cytokine-induced iNOS promoter activity. Also involved in the regulation of IL-8 transcription.
  • 基因功能参考文献：
    1. NKRF is a nucleolar HSP essential for nucleolus homeostasis and cell survival under proteotoxic stress. NKRF is a thermosensor translocating from the nucleolus to the nucleoplasm during heat stress; nucleolar pools are replenished upon HSF1-mediated NKRF resynthesis. NKRF is an unconventional HSP crucial for correct rRNA processing. Under stress conditions, NKRF directs XRN2 nucleolus/nucleoplasm trafficking. PMID: 28096332
    2. Data suggest that L protein from LCMV interactions with host proteome, specifically DDX3X, NKRF, and TRIM21. (LCMV = Lymphocytic choriomeningitis mammarenavirus; DDX3X = DEAD-box helicase 3; NKRF = NF-kappa-B-repressing factor; TRIM21 = tripartite motif-containing protein-21) PMID: 29261807
    3. depletion of NKRF, XRN2 or DHX15 impairs an early pre-rRNA cleavage step. PMID: 28115624
    4. NF-kappaB-repressing factor (NKRF) phosphorylation modulates promoter-proximal transcription elongation of NF-kappaB/NKRF-regulated genes via direct interactions with elongation complex in response to specific stimuli PMID: 26340924
    5. The up-regulated NKRF serves as an endogenous repressor for IP-10 and IL-8 synthesis to hinder host from robust response to MTb infection. PMID: 25135111
    6. MIR29 targets and reduces expression of CLDN1 and NKRF to increase intestinal permeability in inflammatory bowel disease. PMID: 25277410
    7. NRF may serve as an endogenous repressor to prevent robust increase in IP-10/CXCL10 and IL-8/CXCL8 when TB bacterial load is high. PMID: 24223729
    8. The expression of NKRF is decreased in PBMCs of patients with stable COPD. The downregulation of NKRF was found to be closely related to enhanced IL-8 production from these peripheral blood cells through de-repression of the IL-8 promoter. PMID: 22441735
    9. The results indicate that JKTBP1 regulates the level of NRF protein expression by binding to both NRF 5' and 3' UTRs. PMID: 21300069
    10. role of NF-kappa B-repressing factor (NRF) in basal repression of the hiNOS gene PMID: 12381793
    11. cDNA is 3247 bp long, contains three exons and maps to human chromosome Xq24. NRF is widely expressed in human tissues. PMID: 14744032
    12. Full-length NRF is highly enriched in nucleoli and only a small fraction of NRF is found in the nucleoplasm and cytoplasm PMID: 15226370
    13. NRF interrupts the regulatory coupling of LTR binding factors and transcription elongation events; this inhibitory mechanism might contribute to transcriptional quiescence of integrated HIV-1 provirus PMID: 16107696
    14. Induction of MMP-9 gene expression is regulated by oscillatory/cumulative activation of NFkappaB in a colon cancer cell line. PMID: 17186550
    15. The data of this study show that JKTBP1 and the 14-nt element act independently to mediate NRF internal ribosome entry segment activity. PMID: 17592041
    16. saimiri transformation-associated protein of subgroup C induces NF-kappaB activation PMID: 18560378
    17. Induction of NRF in human airway smooth muscle cells by neutrophil elastase mediates the suppression of interleukin (IL)-8/CXCL8 expression. PMID: 19542452

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  • 亚细胞定位：
    Nucleus, nucleolus.
  • 组织特异性：
    Widely and constitutively expressed. Expressed at lower level in colon, peripheral blood lymphocytes, lung and kidney.
  • 数据库链接：

    HGNC: 19374

    OMIM: 300440

    KEGG: hsa:55922

    STRING: 9606.ENSP00000442308

    UniGene: Hs.437084