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  4. Recombinant Human Neuronal calcium sensor 1 (NCS1)

Recombinant Human Neuronal calcium sensor 1 (NCS1)

  • 货号：
    CSB-YP008983HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP008983HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP008983HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP008983HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    NCS1
  • Uniprot No.：
  • 别名：
    9430075O15Rik; A730032G13Rik; AI836659; DKFZp761L1223; FLUP; FREQ; Frequenin; Frequenin homolog (Drosophila); Frequenin homolog; Frequenin like protein; Frequenin, Drosophila, homolog of; Frequenin-like protein; Frequenin-like ubiquitous protein; Mfreq; NCS 1; NCS-1; ncs1; NCS1_HUMAN; Neuronal calcium sensor 1; Neuronal Calicum Sensor 1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-190
  • 氨基酸序列
    GKSNSKLKP EVVEELTRKT YFTEKEVQQW YKGFIKDCPS GQLDAAGFQK IYKQFFPFGD PTKFATFVFN VFDENKDGRI EFSEFIQALS VTSRGTLDEK LRWAFKLYDL DNDGYITRNE MLDIVDAIYQ MVGNTVELPE EENTPEKRVD RIFAMMDKNA DGKLTLQEFQ EGSKADPSIV QALSLYDGLV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Neuronal calcium sensor, regulator of G protein-coupled receptor phosphorylation in a calcium dependent manner. Directly regulates GRK1 (RHOK), but not GRK2 to GRK5. Can substitute for calmodulin. Stimulates PI4KB kinase activity. Involved in long-term synaptic plasticity through its interaction with PICK1. May also play a role in neuron differentiation through inhibition of the activity of N-type voltage-gated calcium channel.
  • 基因功能参考文献：
    1. NCS-1, a calcium-binding protein, is associated with clinicopathologic features of aggressiveness in breast cancer cells and worse outcome in two breast cancer patient cohorts PMID: 28275088
    2. Results show that although NCS-1 Var2mRNA can be detected, protein expression was not detectable. When overexpressed in cell lines NCS-1 Var2 appears to be folded properly, yet it has a lower calcium affinity. Because most NCS-1 binding to its protein partners requires calcium binding, it is unlikely that NCS-1 Var2 would play a major regulatory role in cells. PMID: 27575489
    3. structural and dynamical response of NCS-1 protein to elevated temperature may be one of its intrinsic functional properties PMID: 27007011
    4. Lithium normalizes gamma band oscillations mediated by P/Q-type calcium channels modulated by NCS-1. PMID: 27033453
    5. Study determined the salient features of the folding free-energy landscapes of the Mg2+-bound and apo states of NCS-1 PMID: 26153708
    6. results reveal the important role of salt bridges on the structural properties of NCS-1 protein and that R102Q mutation disables the dynamic relocation of C-terminus, which may block the binding of NCS-1 protein to its receptors PMID: 25343687
    7. single-molecule optical tweezers were used to monitor misfolding reactions of the human neuronal calcium sensor-1. PMID: 25157171
    8. A strong liason was characterized between myristoylation and cryptic EF-1 in NCS-1. PMID: 25565019
    9. The functional link in substantia nigra dopamine neurons between Cav1.3- L-type-Ca2+ channels and D2-autoreceptor activity is an adaptive signalling network controlled by NCS-1. PMID: 24934288
    10. NCS1 has evolved a remarkable complex interdomain cooperativity and a fundamentally different folding mechanism compared to structurally related proteins. PMID: 24012477
    11. data suggest that genetic variants in the NCS-1 gene contribute to susceptibility of Cocaine Abuse in individuals of African descent. PMID: 22999924
    12. [review] Receptor interaction properties in schizophrenia are dependent upon NCS-1, a protein found to regulate the phosphorylation, trafficking, and signaling profile of the D2 dopamine receptor (D2R) in neurons. PMID: 21777187
    13. the C-terminal tail is important for regulating the conformational stability of NCS1 in Ca(2+)-activated state PMID: 22227393
    14. analysis of the interaction between the D2 dopamine receptor and neuronal calcium sensor-1 PMID: 21875085
    15. it is possible to protect cells from paclitaxel-induced degradation of NCS-1 by inhibiting calpain activity PMID: 21808066
    16. NCS-1 is a novel regulator of cardiac calcium signaling, specifically in immature and hypertrophic hearts. PMID: 21737792
    17. Impairment of the normal cycling of NCS-1 by the R102Q mutation could have subtle effects on neuronal signalling and physiology in the developing and adult brain. PMID: 20479890
    18. up-regulated in the prefrontal cortex of schizophrenic and bipolar patients PMID: 12496348
    19. Analysis with chimeric proteins between KChIP2 and NCS-1 reveals that the three regions of KChIP2 are necessary and sufficient for its effective binding to Kv4.3 protein PMID: 12928444
    20. may serve as regulator of certain isoforms of phosphatidylinositol 4-kinase PMID: 14512421
    21. key role for residues within the motif EELTRK in NCS-1 in keeping the myristoyl group exposed and allowing the protein to be constitutively membrane-associated. PMID: 14726528
    22. NCS-1 is shown to be mainly associated with azurophilic granules in human neutrophils and promyelocitic cells and, therefore could play an instrumental role in the calcium-dependent secretion of azurophilic granules PMID: 14760944
    23. These results demonstrate a novel role for NCS-1 and PI4Kbeta in regulating ERK1/2 signaling and inflammatory reactions in mast cells. PMID: 16837555
    24. NCS-1 expression was diminished in CD4+T lymphocytes, CD19+ B lymphocytes and CD14+ monocytes of bipolar disorder patients and also decreased in CD4+ T lymphocytes and CD56+ natural killer cells of schizophrenia patients PMID: 19091302
    25. occurrence of an unusual TG 3' splice site in intron 2 PMID: 17672918

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  • 亚细胞定位：
    Golgi apparatus. Cell junction, synapse, postsynaptic density. Cytoplasm, perinuclear region. Cytoplasm. Cell membrane; Peripheral membrane protein. Membrane; Lipid-anchor.
  • 蛋白家族：
    Recoverin family
  • 数据库链接：

    HGNC: 3953

    OMIM: 603315

    KEGG: hsa:23413

    STRING: 9606.ENSP00000361475

    UniGene: Hs.642946