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  4. Recombinant Human Neuronal migration protein doublecortin (DCX)

Recombinant Human Neuronal migration protein doublecortin (DCX)

  • 中文名称：
    Recombinant Human Neuronal migration protein doublecortin(DCX)
  • 货号：
    CSB-EP006576HU
  • 规格：
    ￥1836
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    DCX
  • Uniprot No.：
  • 别名：
    DBCN; Dbct; DC; DCX; DCX_HUMAN; Doublecortex; Doublin; FLJ51296; Lis X; Lis-X; Lissencephalin X; Lissencephalin-X; Lissencephaly X linked; Lissencephaly X linked doublecortin; LISX; Neuronal migration protein doublecortin; OTTHUMP00000023859; OTTHUMP00000023860; OTTHUMP00000216315; OTTHUMP00000216316; SCLH; XLIS
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length
  • 来源：
    E.coli
  • 分子量：
    65.3kDa
  • 表达区域：
    1-441aa
  • 氨基酸序列
    MKTLPLHSHCTEMQRLLPKLEMLTLGSSFCSLQGEFCQAMDSFTTVSHVGMCEETDASFNVFSPKFQFDRSHCQSLRFHQNMELDFGHFDERDKTSRNMRGSRMNGLPSPTHSAHCSFYRTRTLQALSNEKKAKKVRFYRNGDRYFKGIVYAVSSDRFRSFDALLADLTRSLSDNINLPQGVRYIYTIDGSRKIGSMDELEEGESYVCSSDNFFKKVEYTKNVNPNWSVNVKTSANMKAPQSLASSNSAQARENKDFVRPKLVTIIRSGVKPRKAVRVLLNKKTAHSFEQVLTDITEAIKLETGVVKKLYTLDGKQVTCLHDFFGDDDVFIACGPEKFRYAQDDFSLDENECRVMKGNPSATAGPKASPTPQKTSAKSPGPMRRSKSPADSANGTSSSQLSTPKSKQSPISTPTSPGSLRKHKDLYLPLSLDDSDSLGDSM
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-SUMO-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Microtubule-associated protein required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCLK1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with PAFAH1B1/LIS-1 of overlapping, but distinct, signaling pathways that promote neuronal migration.
  • 基因功能参考文献：
    1. Age-associated expression of the proliferation and immature neuron markers MKI67 and DCX, respectively, was unrelated, suggesting that neurogenesis-associated processes are independently altered at these points in the development from stem cell to neuron. PMID: 28766905
    2. specifically recognizes compacted GDP-like microtubule lattice PMID: 27238282
    3. this suggests that the microtubule-interacting doublecortin domain observed in cryo-electron micrographs is the C-terminal domain rather than the N-terminal one. PMID: 27226599
    4. From this family, we conclude that a DCX mutation causes a pleiotropic phenotype in the female even if X chromosome inactivation pattern is not skewed, and the novel missense mutation in DCX produced relatively mild dysfunction of the doublecortin protein. PMID: 25868952
    5. We identified a novel DCX mutation c.785A > G, p.Asp262Gly in a family with X-linked lissencephaly and subcortical band heterotopia PMID: 27292316
    6. In high-risk metastatic Neuroblastoma, TH and DCX mRNA quantification could be used for the assessment of response to treatment and for early detection of progressive disease or relapses. PMID: 26498952
    7. Results point to a critical role of doublecortin in the formation of the neuromuscular junctions. PMID: 25817838
    8. It was conclude that microtubule ends have two distinct features that proteins can recognize independently, namely a structural feature related to curvature and nucleotide state. PMID: 25283777
    9. DCX-positive cells occur in a wide range of hypothalamic nuclei in humans, mice and sheep. PMID: 24288185
    10. human DCX protein was expressed in human adipose stem cells, collagen II was decreased while aggrecan, matrilin 2, and GDF5 were increased during the 14-day pellet culture. PMID: 24758934
    11. We propose that DCDC2 is a tumor suppressor gene of HCC. PMID: 24034596
    12. in utero doublecortin knockdown, but not knockout, shows a neocortical neuronal migration phenotype. PMID: 24945770
    13. Immunoblots revealed that depressed subjects displayed increased expression of doublecortin. PMID: 23260340
    14. coexpression of DCX and SPARC collaboratively diminished radioresistance of glioma cells. PMID: 23846421
    15. DCX is dispensable for the development of new neurons in adult mice PMID: 23667508
    16. one deleterious mutation in the DCX gene was identified in a 5-year-old girl with lissencephaly spectrum PMID: 23583063
    17. This finding points to the possible implication of mosaic deletions in the DCX gene in unexplained forms of subcortical band heterotopia (SBH) and may allow for detection of SBH prenatally. PMID: 22833188
    18. DCX binds to polymerization intermediates at growing microtubule ends, in support of a mechanism for stabilizing 13-protofilament microtubules. PMID: 22727374
    19. Data report a negative correlation between DCX mRNA expression and white matter neuron density in schizophrenia, in contrast to a positive correlation in human development where DCX mRNA and white matter neuron density are higher earlier in life. PMID: 21966452
    20. Up-regulation of doublecortin is associated with brain damage in children with acute lymphoblastic leukemia PMID: 21846577
    21. DCX synthesis induces apoptosis in brain tumor stem cells (BTSC) through a novel JNK1/neurabin II/DCX/PP1/caspase-3 pathway. PMID: 21477071
    22. study describes male siblings with Lennox-Gastaut syndrome and pachygyria with a novel missense mutation in the DCX gene PMID: 20726879
    23. The DCX binding on the microtubules surface indirectly stabilizes conserved tubulin-tubulin lateral contacts in the microtubules lumen, operating independently of the nucleotide bound to tubulin. PMID: 20974813
    24. variants in doublecortin- and calmodulin kinase like 1, a gene up-regulated by BDNF, have roles in memory and general cognitive abilities PMID: 19844571
    25. Evidence for a role for miR-128 in neuroblastoma progression and aggressiveness through reelin and DCX expression is reported. PMID: 19713529
    26. During development of the cerebral cortex DCX is expressed in both pyramidal and non-pyramidal migrating cells, as well as in Cajal-Retzius cells. PMID: 12427674
    27. Missense DCX mutations may manifest as non-syndromic mental retardation with cryptogenic epilepsy in female subjects and subcortical band heterotopia (SBH) in boys. PMID: 12838518
    28. Different clinical and morphological phenotypes in monozygotic twins with identical DCX mutation. PMID: 14999500
    29. both mutant and wild type DCX are able to bind and bundle microtubules; however, mutants possess a decreased ability to perturb the mitotic machinery, to cause abnormal spindle orientation, and to impair mitotic progression PMID: 15045646
    30. DCX maps at Xq22.3 and is caused by a homozygous mutation. It acts during corticogenesis on radial migratory pathways. PMID: 15057976
    31. We analysed the human DCX regulatory sequence and confined it to a 3.5-kb fragment upstream of the ATG start codon. This fragment is sufficient and specific to drive expression of reporter genes in embryonic and adult neuronal precursors PMID: 15663475
    32. doublecortin (DCX) gene as a new molecular marker of neuroblastoma cells PMID: 15714065
    33. Collectively, the immunohistochemistry, Western blots and Northern blots conclusively demonstrate expression of DCX by human brain tumors. PMID: 16195916
    34. Doublecortin can be regarded as specific neuronal marker only in normal developing brain, but lacks specificity in glioneuronal and glial tumours and other non-neuronal human tissues where it is expressed in a wide variety of tumours and tissues. PMID: 16520969
    35. X-ray structure of a mutant of N-DCX, in which the C-terminal fragment (residues 139-147) unexpectedly shows an altered, "open" conformation PMID: 16835924
    36. Our data in the mouse, identifying roles for Dcx in hippocampal and corpus callosal development, might suggest intrinsic roles for human DCX in the development of these structures. PMID: 17111359
    37. Doublecortin (DCX) is one of the three genes found from Affymetrix gene chip analysis related to glioma patient survival. PMID: 17178868
    38. MLPA uncovers large genomic deletions of the DCX gene in a subset of patients with SBH in whom no mutations are found after gene sequencing. Deletions of DCX are an underascertained cause of SBH. PMID: 17283321
    39. Results demonstrate that DCX can be used as a marker to distinguish articular chondrocytes from other chondrocytes and to evaluate the quality of tissue engineered or regenerated cartilage in terms of their "articular" or "non-articular" nature. PMID: 17897623
    40. Doublecortin is applicable for the detection of individual infiltrating glioma cells when combined with other markers. PMID: 18415660
    41. X-linked lissencephaly patients with mutations in the C-DC domain tended to have a less severe lissencephaly (grade 4-5 in 58.3%) compared with those in the N-DC domain (grade 4-5 in 36.3%. PMID: 18685874
    42. intragenic deletions and duplications of the DCX gene account for a significant number of patients with isolated lissencephaly sequence and subcortical band heterotopia, where no molecular defect had previously been identified. PMID: 19050731
    43. Doublecortin (DCX) upregulation, correlates with better neurologic outcome in children following traumatic head injury. PMID: 19221293

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  • 相关疾病：
    Lissencephaly, X-linked 1 (LISX1); Subcortical band heterotopia X-linked (SBHX)
  • 亚细胞定位：
    Cytoplasm. Cell projection, neuron projection.
  • 组织特异性：
    Highly expressed in neuronal cells of fetal brain (in the majority of cells of the cortical plate, intermediate zone and ventricular zone), but not expressed in other fetal tissues. In the adult, highly expressed in the brain frontal lobe, but very low ex
  • 数据库链接：

    HGNC: 2714

    OMIM: 300067

    KEGG: hsa:1641

    STRING: 9606.ENSP00000337697

    UniGene: Hs.34780