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  4. Recombinant Human Nuclear inhibitor of protein phosphatase 1 (PPP1R8)

Recombinant Human Nuclear inhibitor of protein phosphatase 1 (PPP1R8)

  • 货号：
    CSB-EP618641HU
  • 规格：
    ￥1344
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    PPP1R8
  • Uniprot No.：
  • 别名：
    Activator of RNA decay; ARD 1; ARD-1; ARD1; Homolog of E.coli RNase E; NIPP 1; NIPP-1; NIPP1; Nuclear inhibitor of protein phosphatase 1; nuclear inhibitor of protein phosphatase-1 alpha; Nuclear subunit of PP1; PP1R8_HUMAN; PPP1R8; PRO2047 ; Protein phosphatase 1 regulatory inhibitor subunit 8; Protein phosphatase 1 regulatory subunit 8; RNase E; RNase E, E. coli, homolog of
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Isoform 2
  • 来源：
    E.coli
  • 分子量：
    52.1kDa
  • 表达区域：
    1-209aa
  • 氨基酸序列
    MGGEDDELKGLLGLPEEETELDNLTEFNTAHNKRISTLTIEEGNLDIQRPKRKRKNSRVTFSEDDEIINPEDVDPSVGRFRNMVQTAVVPVKKKRVEGPGSLGLEESGSRRMQNFAFSGGLYGGLPPTHSEAGSQPHGIHGTALIGGLPMPYPNLAPDVDLTPVVPSAVNMNPAPNPAVYNPEAVNEPKKKKYAKEAWPGKKPTPSLLILENGTHMASSDACHECKSMIAAAG
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal GST-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Inhibitor subunit of the major nuclear protein phosphatase-1 (PP-1). It has RNA-binding activity but does not cleave RNA and may target PP-1 to RNA-associated substrates. May also be involved in pre-mRNA splicing. Binds DNA and might act as a transcriptional repressor. Seems to be required for cell proliferation.; Isoform Gamma is a site-specific single-strand endoribonuclease that cleaves single strand RNA 3' to purines and pyrimidines in A+U-rich regions. It generates 5'-phosphate termini at the site of cleavage. This isoform does not inhibit PP-1. May be implicated in mRNA splicing.
  • 基因功能参考文献：
    1. NIPP1 could be activated by hypoxia and contributed to hypoxia-induced invasive and metastatic potential in hepatocellular carcinoma. PMID: 27644248
    2. Data suggest overexpression of NIPP1 in HeLa cells causes induction of genes of mesenchymal lineage with reduction in cell proliferation; thus, key dephosphorylation events may be involved in epithelial-mesenchymal transition or transdifferentiation. PMID: 25907536
    3. The protein phosphatase-1 regulator NIPP1 is also a splicing factor involved in a late step of spliceosome assembly PMID: 11909864
    4. NIPP1 has a role in the nuclear targeting and/or retention of PP1 PMID: 15501817
    5. Required for the global trimethylation of histone3 at lysine27 and is implicated in gene silencing by enhancer of zeste homolog (EZH)2. PMID: 17724462
    6. NIPP1 is present in a complex with EED and EZH2 in vivo and has distinct binding sites for these proteins. PMID: 17804093
    7. NMR structure of the NIPP1 FHA domain. PMID: 18253837
    8. NIPP1 works as a molecular sensor for PP1 to recognize phosphorylated Sap155. PMID: 18842582

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  • 亚细胞定位：
    Nucleus. Nucleus speckle. Note=Primarily, but not exclusively, nuclear.; [Isoform Gamma]: Cytoplasm. Note=Found mainly in the cytoplasm.
  • 组织特异性：
    Ubiquitously expressed, with highest levels in heart and skeletal muscle, followed by brain, placenta, lung, liver and pancreas. Less abundant in kidney. The concentration and ratio between isoforms is cell-type dependent. Isoform Alpha (>90%) and isoform
  • 数据库链接：

    HGNC: 9296

    OMIM: 602636

    KEGG: hsa:5511

    STRING: 9606.ENSP00000311677

    UniGene: Hs.533474