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  4. Recombinant Human Nuclear receptor subfamily 1 group I member 3 (NR1I3)

Recombinant Human Nuclear receptor subfamily 1 group I member 3 (NR1I3)

  • 货号：
    CSB-YP622930HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP622930HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP622930HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP622930HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    NR1I3
  • Uniprot No.：
  • 别名：
    CAR; CAR BETA; CAR1; CARA; Constitutive activator of retinoid response; constitutive active receptor; Constitutive active response; constitutive androstane nuclear receptor variant 2; constitutive androstane nuclear receptor variant 3; constitutive androstane nuclear receptor variant 4; constitutive androstane nuclear receptor variant 5; Constitutive androstane receptor; MB67; MGC97144; NR1I3; NR1I3_HUMAN; Nuclear receptor subfamily 1 group I member 3; orphan nuclear hormone receptor; Orphan nuclear receptor MB67; Orphan nuclear receptor NR1I3
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-352
  • 氨基酸序列
    MASREDELRN CVVCGDQATG YHFNALTCEG CKGFFRRTVS KSIGPTCPFA GSCEVSKTQR RHCPACRLQK CLDAGMRKDM ILSAEALALR RAKQAQRRAQ QTPVQLSKEQ EELIRTLLGA HTRHMGTMFE QFVQFRPPAH LFIHHQPLPT LAPVLPLVTH FADINTFMVL QVIKFTKDLP VFRSLPIEDQ ISLLKGAAVE ICHIVLNTTF CLQTQNFLCG PLRYTIEDGA RVSPTVGFQV EFLELLFHFH GTLRKLQLQE PEYVLLAAMA LFSPDRPGVT QRDEIDQLQE EMALTLQSYI KGQQRRPRDR FLYAKLLGLL AELRSINEAY GYQIQHIQGL SAMMPLLQEI CS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Binds and transactivates the retinoic acid response elements that control expression of the retinoic acid receptor beta 2 and alcohol dehydrogenase 3 genes. Transactivates both the phenobarbital responsive element module of the human CYP2B6 gene and the CYP3A4 xenobiotic response element.
  • 基因功能参考文献：
    1. Expression of microRNA potentially regulated by AhR and CAR in malignant tumors of the endometrium has been reported. PMID: 30225717
    2. Phosphomimetic substitution of Thr-38 with Asp increased co-immunoprecipitation of the CAR DNA Binding Domain with CAR Ligand Binding Domain in Huh-7 cells. The underlying molecular mechanism that regulates CAR activation is its homodimer-monomer-heterodimer conversion; the monomer and homodimer are phosphorylated at Thr-38 and inactive. PMID: 29133527
    3. Results suggest that TRIM24 is a novel coactivator of the CAR that is involved in cell- and/or promoter- selective transactivation. PMID: 29101097
    4. An overview of NR1I2 and NR1I3 pharmacogenetic studies in various therapeutic fields. PMID: 29199543
    5. replication confirmed at genome-wide significance the association of loci at FOXE1 with hypothyroidism, and PDE8B, CAPZB and PDE10A with serum TSH. A total of 12 SNPs seemed to explain nearly 7% of the serum TSH variation PMID: 28777242
    6. Dual ligands of CAR/PXR show distinct gene regulation patterns by regulating cross-talk between CAR and PXR. PMID: 27197997
    7. Data suggest both PXR and CAR are expressed in testis/Sertoli cells; exposure of Sertoli cells (in vitro model of blood-testis barrier) to PXR or CAR ligands (including antiretroviral drugs) up-regulates expression of Pgp/ABCB1, BCRP, and MRP4. (PXR = pregnane X receptor; CAR = constitutive androstane receptor; Pgp/ABCB1 = P-glycoprotein ABCB1; BCRP = breast cancer resistance protein; MRP4 =multidrug resistance protein 4) PMID: 28970358
    8. Genome-wide comparison of the inducible transcriptomes of nuclear receptors CAR, PXR and PPARalpha in primary human hepatocytes has been presented. PMID: 26994748
    9. These data provide new insights into the regulation by CAR of glycolytic and lipogenic genes and on pathogenesis of steatosis PMID: 27180240
    10. Integrative analysis of four RNA-Seq datasets and differential expression revealed for the first time, splicing alterations of SLC39A14 and NR1I3 in hepatocellular carcinoma. PMID: 26711644
    11. These results reveal both novel and known targets of hCAR and support the role of hCAR in maintaining the homeostasis of metabolism and cell proliferation in the liver. PMID: 26275810
    12. The homozygous variant genotype of MPJ6_1I3008 is associated with a significant reduced risk of neonatal hyperbilirubinemia in females. PMID: 26188155
    13. Around 42.5% of the overall interindividual variability in warfarin dose requirements was explained : VKORC1 genotype accounted for 29.6%, CYP2C9 genotype for 4.3%, age for 3.6%, the CYP4F2 genotype for 3.3%, and CAR/HNF4alpha (rs2501873/rs3212198) for 1.7% PMID: 25356900
    14. It is suggested that the functions of PXR, CAR and AhR may be closely implicated in the pathogeneses of metabolic vascular diseases, such as hyperlipidemia, atherogenesis, and hypertension. PMID: 24859622
    15. Suggest that PXR and CAR double humanized mice are more sensitive rifampcin induction of cytochrome P450 and UDP-glucuronosyltransferases. PMID: 25835148
    16. PRMT5 enhances transcriptional activity of constitutive androstane receptor.PRMT5 is a gene-selective co-activator for CAR. PMID: 25721668
    17. The nuclear receptor constitutive androstane receptor/NR1I3 enhances the profibrotic effects of transforming growth factor beta and contributes to the development of experimental dermal fibrosis. PMID: 25155144
    18. Donor CYP3A5, NR1I3 gene polymorphisms. PMID: 24351870
    19. ubiquitin-proteasomal regulation of CCRP and HSP70 are important contributors to the regulation of cytoplasmic CAR levels, and hence the ability of CAR to respond to PB or PB-like inducers PMID: 24789201
    20. The polymorphisms investigated in PPARA (rs1800206), RXRA (rs11381416), and NR1I2 (rs1523130) did not influence the lipid-lowering efficacy and safety of statin and our results show the possible influence of NR1I3 genetic variant on the safety of statin. PMID: 24232815
    21. miR-137 is a crucial regulator of cancer response to doxorubicin treatment PMID: 23934188
    22. The CAR variant, which results in a poor metabolizer phenotype, could account for a higher degree of external genitalia virilization in 21OHD females. PMID: 22978668
    23. Gadd45B protects the liver through two entirely different processes: binding MKK7 to block damaging signal transduction or binding CAR to coactivate anabolic transcription. (Review) PMID: 24104474
    24. Activated p38 MAPK is required for CAR to selectively activate a set of genes that encode drug-metabolizing enzymes. PMID: 23539296
    25. Common genetic variants in the xenobiotic transport and metabolism regulator genes PXR and CAR do not have a significant association with multiple myeloma risk. PMID: 23303387
    26. CYP2B6 516G>T (P < 0.0001) and CAR rs2307424 C>T (P = 0.002) were significantly related to efavirenz plasma concentrations. PMID: 23172109
    27. Constitutive androstane receptor-mediated enzyme induction in the liver depends on the presence of beta-catenin at the time of xenobiotic treatment. PMID: 23578392
    28. Report CAR expression in human parotid glands. PMID: 23614276
    29. Polymorphisms in the RXRA and NR1I3 genes influence lipid profile in a Southern Brazilian population. PMID: 23079705
    30. DAX-1 as a novel and potent constitutive androstane receptor (CAR) corepressor and suggest that DAX-1 functions as a coordinate hepatic regulator of CAR's biological function. PMID: 22896671
    31. DP97 was found to be a gene (or promoter)-selective co-activator for hCAR PMID: 22910411
    32. phenobarbital-mediated down-regulation of miR-122 is an early and important event in the AMPK-dependent CAR activation and transactivation of its target genes PMID: 22815988
    33. In severe intrahepatic cholestasis of pregnancy the CAR expression in placenta syncytiotrophoblastic cells increased appreciably, which may be involved in the maintenance of placenta barrier function and protection. PMID: 21733368
    34. CAR transactivates ABCG2 through the DR5 motif located in its distal promoter in hepatocytes and the motif prefers CAR to pregnane X receptor. PMID: 22093699
    35. activated ERK1/2 interacts with CAR and represses dephosphorylation of Thr-38, providing a cell signal-regulated mechanism for CAR activation. PMID: 21873423
    36. CAR mRNA levels were unchanged while protein levels were markedly induced in obstructive cholestasis as compared with the controls. PMID: 21359593
    37. These data indicate that genetic variability in CYP2B6 and constitutive androstane receptor contributes to early treatment discontinuation for efavirenz-based antiretroviral regimens PMID: 21715435
    38. These findings provide the first evidence for P-glycoprotein regulation by pregnane X receptor and constitutive androstane receptor at the human blood brain barrier. PMID: 21517853
    39. results suggest that HNF4alpha plays an important role in the constitutive expression of hepatic UGT2B7, and CAR acts as a negative regulator by interfering with HNF4alpha binding activity PMID: 21415305
    40. constitutive androstane receptor (hCAR) mediated the CYP2B6 induction by cigarette smoke extract in Hep2G cells; data suggest that smoking up-regulates CYP2B6 through hCAR in vivo PMID: 20966044
    41. FXR activation in obstructive cholestasis might worsen liver injury by hijacking a protective mechanism regulated by CAR and provides a new molecular explanation to the pathophysiology of cholestasis. PMID: 21296199
    42. CCAAT/enhancer-binding protein alpha (C/EBPalpha) and hepatocyte nuclear factor 4alpha (HNF4alpha) synergistically cooperate with constitutive androstane receptor to transactivate the human cytochrome P450 2B6 (CYP2B6) gene PMID: 20622021
    43. Four haplotypes were determined for CAR single nucleotide polymorphisms. PMID: 20218903
    44. Data observed that OSM positively augmented the CAR and UGT1A1 expressions and CAR-mediated signaling in vivo and in vitro, through cross talk between the nuclear CAR receptor and the plasma membrane OSM receptor, via the MAPK cascade. PMID: 20197307
    45. Results characterize the conserved threonine 38 of human constitutive active/androstane receptor (CAR) as the primary residue that regulates nuclear translocation and activation of CAR. PMID: 19858220
    46. CAR antagonizes ER-mediated transcriptional activity by squelching limiting amounts of p160 coactivators, such as SRC-1 and GRIP-1. PMID: 12114525
    47. Transcriptional regulation of the human CYP3A4 gene by the constitutive androstane receptor. PMID: 12130689
    48. Regulation of human CYP2C9 by the constitutive androstane receptor: discovery of a new distal binding site. PMID: 12181452
    49. identified as a key regulator of acetaminophen metabolism and hepatotoxicity PMID: 12376703
    50. Substantial interindividual differences exist in hepatic constitutive androstane receptor expression; a 240-fold interindividual variability in hepatic mRNA levels has been detected. PMID: 12485946

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  • 亚细胞定位：
    Nucleus. Cytoplasm. Cytoplasm, cytoskeleton.
  • 蛋白家族：
    Nuclear hormone receptor family, NR1 subfamily
  • 组织特异性：
    Predominantly expressed in liver.
  • 数据库链接：

    HGNC: 7969

    OMIM: 603881

    KEGG: hsa:9970

    STRING: 9606.ENSP00000356958

    UniGene: Hs.349642