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  4. Recombinant Human Nuclear receptor subfamily 5 group A member 2 (NR5A2)

Recombinant Human Nuclear receptor subfamily 5 group A member 2 (NR5A2)

  • 货号：
    CSB-YP016066HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP016066HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP016066HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP016066HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP016066HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    NR5A2
  • Uniprot No.：
  • 别名：
    Alpha-1-fetoprotein transcription factor; B1-binding factor; B1F; B1F2; CPF; CYP7A promoter-binding factor; FTF; FTZ F1; FTZ F1beta; FTZ-F1; FTZ-F1beta; hB1F 2; hB1F; hB1F-2; Hepatocytic transcription factor; Liver receptor homolog 1; LRH-1; LRH1; Nr5a2; NR5A2_HUMAN; Nuclear receptor subfamily 5 group A member 2
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-541
  • 氨基酸序列
    MSSNSDTGDL QESLKHGLTP IGAGLPDRHG SPIPARGRLV MLPKVETEAL GLARSHGEQG QMPENMQVSQ FKMVNYSYDE DLEELCPVCG DKVSGYHYGL LTCESCKGFF KRTVQNNKRY TCIENQNCQI DKTQRKRCPY CRFQKCLSVG MKLEAVRADR MRGGRNKFGP MYKRDRALKQ QKKALIRANG LKLEAMSQVI QAMPSDLTIS SAIQNIHSAS KGLPLNHAAL PPTDYDRSPF VTSPISMTMP PHGSLQGYQT YGHFPSRAIK SEYPDPYTSS PESIMGYSYM DSYQTSSPAS IPHLILELLK CEPDEPQVQA KIMAYLQQEQ ANRSKHEKLS TFGLMCKMAD QTLFSIVEWA RSSIFFRELK VDDQMKLLQN CWSELLILDH IYRQVVHGKE GSIFLVTGQQ VDYSIIASQA GATLNNLMSH AQELVAKLRS LQFDQREFVC LKFLVLFSLD VKNLENFQLV EGVQEQVNAA LLDYTMCNYP QQTEKFGQLL LRLPEIRAIS MQAEEYLYYK HLNGDVPYNN LLIEMLHAKR A
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Nuclear receptor that acts as a key metabolic sensor by regulating the expression of genes involved in bile acid synthesis, cholesterol homeostasis and triglyceride synthesis. Together with the oxysterol receptors NR1H3/LXR-alpha and NR1H2/LXR-beta, acts as an essential transcriptional regulator of lipid metabolism. Plays an anti-inflammatory role during the hepatic acute phase response by acting as a corepressor: inhibits the hepatic acute phase response by preventing dissociation of the N-Cor corepressor complex. Binds to the sequence element 5'-AACGACCGACCTTGAG-3' of the enhancer II of hepatitis B virus genes, a critical cis-element of their expression and regulation. May be responsible for the liver-specific activity of enhancer II, probably in combination with other hepatocyte transcription factors. Key regulator of cholesterol 7-alpha-hydroxylase gene (CYP7A) expression in liver. May also contribute to the regulation of pancreas-specific genes and play important roles in embryonic development. Activates the transcription of CYP2C38.
  • 基因功能参考文献：
    1. Lrh-1 transcriptionally regulates Oat2. PMID: 29669824
    2. Rev-erbalpha regulates Cyp7a1 and cholesterol metabolism through its repression of the Lrh-1 receptor. PMID: 29237721
    3. transcriptional regulation by NR5A2 links differentiation and inflammation in the pancreas; findings support the notion that, in the pancreas, the transcriptional networks involved in differentiation-specific functions also suppress inflammatory programs; under conditions of genetic or environmental constraint, these networks can be subverted to foster inflammation PMID: 29443959
    4. our data indicated that miR-381 inhibited migration and invasion of non-small cell lung cancer (NSCLC)by targeting LRH-1, and may represent a novel potential therapeutic target and prognostic marker for NSCLC. PMID: 29048619
    5. NR5A2-mediated cancer cell survival is facilitated through augmentation of GATA6 and anti-apoptotic factor BCL-XL levels. PMID: 27586588
    6. the expression level of LRH-1 can be used as a marker in the early diagnosis of unexplained recurrent spontaneous abortion. PMID: 28531169
    7. Liver receptor homologue1 (LRH1) is a direct target of miR30d in colorectal carcinoma cells. PMID: 28440426
    8. Results show that LRH-1 is a direct target gene of miR-374b and that decreased miR-374b expression may contribute to the promotion of LRH-1-mediated tumorigenesis of colon cancer. PMID: 29128635
    9. NR5A2 may be important in the pathophysiology of preterm birth and exploring noncoding regulators of NR5A2 is warranted PMID: 26761123
    10. Our study suggests that miR-219-5p regulated the proliferation, migration, and invasion of human gastric cancer cells by suppressing LRH-1. PMID: 27983934
    11. Liver receptor homolog-1 was identified as a direct target gene of miR-136 PMID: 28710032
    12. miR-27b-3p levels were found to be significantly negatively correlated with both NR5A2 and CREB1 levels in breast cancer tissues. PMID: 27809310
    13. The fusion transcript NR5A2-KLHL29FT was identified in normal and cancerous colonic epithelia. It is due to an uncharacterized polymorphic germline insertion of the NR5A2 sequence from chromosome 1 into the KLHL29 locus at chromosome 2, rather than a chromosomal rearrangement. NR5A2-KLH29FT expression levels were significantly lower in colon cancers than in matched normal colonic epithelia. PMID: 28081303
    14. Additionally, the s solved the structure of the human LRH-1 DNA-binding domain bound to a DR0 motif located within the Oct4 promoter. PMID: 27984042
    15. miR-376c inhibits non-small-cell lung cancer cell growth and invasion by targeting LRH-1 PMID: 27049310
    16. The first crystal structure of the LRH-1-PGC1alpha complex , which depicts hydrophobic contacts is identified and described. PMID: 28363985
    17. The dramatic repositioning is influenced by a differential ability to establish stable face-to-face pi-pi-stacking with the LRH-1 residue His-390, as well as by a novel polar interaction mediated by the RJW100 hydroxyl group. The differing binding modes result in distinct mechanisms of action for the two agonists. PMID: 27694446
    18. The role of NR5A2 in regulating pancreatic cancer stem cell properties and epithelial-mesenchymal transition of pancreatic carcinoma cells PMID: 27996162
    19. demonstrate aberrant expressions of SF-1 and LRH-1 in endometriotic granulosa-lutein cells PMID: 26530052
    20. Down-regulation of MicroRNA-381 promotes cell proliferation and invasion in colon cancer cells through up-regulation of LRH-1. PMID: 26320367
    21. The present study indicates that miR-381 may be a novel tumor suppressor that blocks HCC growth and invasion by targeting LRH-1. PMID: 26677080
    22. The SNPs rs3790843 and rs3790844 in the NR5A2 gene are associated with pancreatic cancer risk in Japanese subjects. PMID: 26592175
    23. SERBP1 is a component of the LRH-1 transcriptional complex. PMID: 26398198
    24. These findings demonstrate that in vitro LRH-1 can act like SF-1 and compensate for its deficiency. PMID: 25896302
    25. These findings show that POD-1/TCF21 regulates SF-1 and LRH-1 by distinct mechanisms, contributing to the understanding of POD-1 involvement and its mechanisms of action in adrenal and liver tumorigenesis. PMID: 26421305
    26. Studies indicate that liver receptor homolog-1 (LRH-1) is critical involvement in multiple types of cancer, and represents a desirable target for therapeutic applications. PMID: 25951367
    27. in spermatozoa the LRH-1 effects are closely integrated with the estrogen signaling, supporting LRH-1 as a downstream effector of the estradiol pathway on some sperm functions. PMID: 26241668
    28. LRH-1 drives colon cancer cell growth by repressing the expression of the CDKN1A gene in a p53-dependent manner. PMID: 26400164
    29. Data suggest LRH1/NR5A2 exhibits phospholipid-mediated allosteric control of protein-protein binding interface in interactions with TIF2 (co-activator; transcription intermediary factor 2) and SHP (co-repressor; small heterodimer partner protein). PMID: 26553876
    30. Results identify LRH-1 as a critical component of the anti-inflammatory and fungicidal response of alternatively activated macrophages that acts upstream from the IL-13-induced 15-HETE/PPARgamma axis. PMID: 25873311
    31. AFPR may play a pivotal role in HBV-related hepatocarcinogenesis. PMID: 25943101
    32. our findings present supportive evidence that ApoM is a regulator of human LRH-1 transcription, and further reveal the importance of ApoM as a critical regulator of bile acids metabolism PMID: 25987835
    33. these data demonstrate that copper-mediated nuclear receptor dysfunction disrupts liver function in WD and potentially in other disorders associated with increased hepatic copper levels. PMID: 26241054
    34. Analysis of breast cancer samples reveals that a high LRH-1 level is inversely correlated with CDKN1A expression in breast cancer patients and is associated with poor prognosis PMID: 25435372
    35. loss of LRH-1 by siRNA or miR-451 mimics significantly impaired Wnt/beta-catenin activity, leading to G0/G1 cell cycle arrest PMID: 25869073
    36. the NR5A2 rs3790844 polymorphism is associated with increased OS of GC patients in the dominant model, and similar results were found among the female group and tumor size >5 cm group for NR5A2 rs3790843 polymorphism. PMID: 25514243
    37. This study demonstrates a critical proproliferative role for LRH-1 in established colon cancer cell lines. PMID: 25675535
    38. during chronic colitis, TNF suppresses intestinal steroidogenic gene expression by inhibiting the activity of NR5A2, thus decreasing glucocorticoid synthesis and sustaining chronic inflammation. PMID: 24570488
    39. LRH1 overexpression is associated with increased pancreatic cancer growth and metastatic spread PMID: 24769073
    40. Data (including data from transgenic overexpression/gene silencing) suggest that NR5A2 modulates signal transduction/cell proliferation in mammary cells; mammary morphology exhibits significant reduction in lateral budding after NR5A2 overexpression. PMID: 24564400
    41. report the genome-wide location and molecular function of LRH-1 in breast cancer cells and reveal its therapeutic potential for the treatment of breast cancers, notably for tumors resistant to treatments currently used in therapies. PMID: 24520076
    42. Data conclude that SF-1 regulates aromatase expression in GCT; over-expression of LRH-1 suggests that this receptor may be involved in the pathogenesis of GCT by mechanisms other than the regulation of aromatase. PMID: 23537609
    43. Lrh-1 is necessary for maintenance of the corpus luteum, for promotion of decidualization and for formation of the placenta. PMID: 23817023
    44. Data indicate that sequence divergence has differentially impacted ligand binding and protein dynamics in NR5A2. PMID: 23737522
    45. Experimental evaluation of the predicted ligands identified two compounds that inhibit the transcriptional activity of LRH-1 and diminish the expression of the receptor's target genes. PMID: 23667258
    46. Expression of human LRH-1 is regulated in a tissue-specific manner, and that the novel promoter region is controlled by the Sp-family, NR5A-family and PGC-1alpha in ovarian granulosa cells in a coordinated fashion. PMID: 23471216
    47. this study indicates that LRH-1 acts as a transcriptional activator in the regulation of OCT4 gene expression through the cooperative interaction with three binding sites directly or/and indirectly. PMID: 23000165
    48. HNF4alpha and LRH-1 promote active transcription histone marks on the Cyp7a1 promoter that are reversed by FGF19 in a SHP-dependent manner PMID: 23038264
    49. The lipid-free receptor undergoes previously unrecognized structural fluctuations, allowing it to interact with widely expressed co-repressors. PMID: 22504882
    50. Data show that single nucleotide polymorphisms (SNPs) in ABO, sonic hedgehog (SHH), telomerase reverse transcriptase (TERT), nuclear receptor subfamily 5, group A, member 2 (NR5A2) were found to be associated with pancreatic cancer risk. PMID: 22125638

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Nuclear hormone receptor family, NR5 subfamily
  • 组织特异性：
    Abundantly expressed in pancreas, less in liver, very low levels in heart and lung. Expressed in the Hep-G2 cell line. Isoform 1 and isoform 2 seem to be present in fetal and adult liver and Hep-G2 cells.
  • 数据库链接：

    HGNC: 7984

    OMIM: 604453

    KEGG: hsa:2494

    STRING: 9606.ENSP00000356331

    UniGene: Hs.33446