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  4. Recombinant Human Nucleolar protein 3 (NOL3)

Recombinant Human Nucleolar protein 3 (NOL3)

  • 中文名称：
    人NOL3重组蛋白
  • 货号：
    CSB-EP015921HU
  • 规格：
    ￥1344
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    NOL3
  • Uniprot No.：
  • 别名：
    Apoptosis repressor with CARD; ARC; Muscle enriched cytoplasmic protein; Muscle-enriched cytoplasmic protein; MYC; MYP; Nol3; NOL3_HUMAN; NOP; Nop30; Nucleolar protein 3 (apoptosis repressor with CARD domain) ; Nucleolar protein 3; Nucleolar protein of 30 kDa
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Isoform 2
  • 来源：
    E.coli
  • 分子量：
    38.6kDa
  • 表达区域：
    1-208aa
  • 氨基酸序列
    MGNAQERPSETIDRERKRLVETLQADSGLLLDALLARGVLTGPEYEALDALPDAERRVRRLLLLVQGKGEAACQELLRCAQRTAGAPDPAWDWQHVGPGYRDRSYDPPCPGHWTPEAPGSGTTCPGLPRASDPDEAGGPEGSEAVQSGTPEEPEPELEAEASKEAEPEPEPEPELEPEAEAEPEPELEPEPDPEPEPDFEERDESEDS
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-SUMO-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    May be involved in RNA splicing.; Functions as an apoptosis repressor that blocks multiple modes of cell death. Inhibits extrinsic apoptotic pathways through two different ways. Firstly by interacting with FAS and FADD upon FAS activation blocking death-inducing signaling complex (DISC) assembly. Secondly by interacting with CASP8 in a mitochondria localization- and phosphorylation-dependent manner, limiting the amount of soluble CASP8 available for DISC-mediated activation. Inhibits intrinsic apoptotic pathway in response to a wide range of stresses, through its interaction with BAX resulting in BAX inactivation, preventing mitochondrial dysfunction and release of pro-apoptotic factors. Inhibits calcium-mediated cell death by functioning as a cytosolic calcium buffer, dissociating its interaction with CASP8 and maintaining calcium homeostasis. Negatively regulates oxidative stress-induced apoptosis by phosphorylation-dependent suppression of the mitochondria-mediated intrinsic pathway, by blocking CASP2 activation and BAX translocation. Negatively regulates hypoxia-induced apoptosis in part by inhibiting the release of cytochrome c from mitochondria in a caspase-independent manner. Also inhibits TNF-induced necrosis by preventing TNF-signaling pathway through TNFRSF1A interaction abrogating the recruitment of RIPK1 to complex I. Finally through its role as apoptosis repressor, promotes vascular remodeling through inhibition of apoptosis and stimulation of proliferation, in response to hypoxia. Inhibits too myoblast differentiation through caspase inhibition.
  • 基因功能参考文献：
    1. Results show that in response to DNA damage, p53 total levels increase proportionally to the strength of the damage; however, p53 tetramers are formed at a constant rate under the control of ARC protein. PMID: 25344068
    2. role of apoptosis repressor with a CARD domain (ARC) in the therapeutic resistance of renal cell carcinoma PMID: 28464919
    3. a novel genetic primary myelofibrosis-like mouse model and identify a tumor suppressor role for NOL3 in the pathogenesis of myeloid malignancies. PMID: 28232469
    4. Increased ARC expression is associated with liver metastasis of colorectal cancer. PMID: 26721253
    5. RUNX3, miR-185 and ARC regulate the sensitivity of gastric cancer cells to chemotherapy. PMID: 24763054
    6. ARC is regulated via BIRC2/MAP3K14 signalling and its overexpression in AML or MSCs can function as a resistant factor to birinapant-induced leukaemia cell death. PMID: 25079338
    7. high expression of ARC plays an important role in the pathogenesis of nasopharyngeal carcinoma and leads to X-radiation and cisplatin resistance in nasopharyngeal carcinoma. PMID: 23877130
    8. ARC is a previously unrecognized inhibitor of apoptosis in beta-cells and that its protective effects are mediated through suppression of the ER stress response pathway. PMID: 22933109
    9. This study utilized unbiased, genome-wide approaches to identify a NOL3 mutation that likely causes Familial cortical myoclonus. PMID: 22926851
    10. HIF-1alpha directly bound to hypoxia-responsive element located at -419 to -414 of ARC gene, which is essential for HIF-1-induced expression. PMID: 22475487
    11. Data show that ARC promotes breast carcinogenesis by driving primary tumor growth, invasion, and metastasis as well as by promoting chemoresistance in invasive cells. PMID: 22037876
    12. ARC, previously unlinked to pulmonary hypertension, is a critical determinant of vascular remodeling in this syndrome. PMID: 22082675
    13. Results suggest that ARC expression levels are highly prognostic in AML and that ARC is a potential therapeutic target in AML. PMID: 21041716
    14. Ras induces ARC in epithelial cancers, and ARC plays a role in the oncogenic actions of Ras PMID: 20392691
    15. These results suggest that the antiapoptotic effect of apoptotic repressor with caspase recruitment domain is, in part, due to inhibition of voltage-gated potassium channels in cardiomyocytes. PMID: 12734105
    16. calcium binding mediates regulation of caspase 8 and cell death by ARC PMID: 15509781
    17. Unexpectedly, ARC was localized almost exclusively to the nuclei of cancer cells, which was unlike the cytoplasmic localization of ARC in non-cancer cells PMID: 15848180
    18. ARC was present in the cytoplasm and nuclei of epithelial cells in invasive ductal carcinoma PMID: 15861191
    19. is downregulated in human failing myocardium PMID: 16505176
    20. nuclear apoptosis repressor with caspase recruitment domain (ARC)is induced in cancer cells and negatively regulates p53 PMID: 18087040
    21. the high level of ARC protein and the constitutive phosphorylation of ARC in cancer cells may play an important role in the protection of cancer cells against oxidative stress PMID: 18172857
    22. Transfection of cDNA encoding ARC into Me1007 cells inhibited both caspase-8 activation and apoptosis induced by thapsigargin or tunicamycin. PMID: 18245485
    23. ARC is a novel marker of human colon cancer and suggest that it may be a general feature of epithelial cancers. PMID: 18469522
    24. the balance between antiapoptotic ARC and proapoptotic caspase-8 is the only one to be disturbed during carcinogenesis and tumour progression of renal cell carcinomas PMID: 18516683
    25. ARC undergoes poly-ubiquitination and subsequent proteasome-dependent degradation. Mutation of ARC's lysine residues prevents this and enhances its pro-survival effects. PMID: 17142452
    26. ARC holds multiple death pathways in check by non-homotypic death-fold interactions. Loss of ARC disinhibits these, leading to accelerated DISC assembly and Bax activation and may be an apoptotic trigger in heart failure and ischemia-reperfusion. PMID: 15383280
    27. The CARD of ARC binds the Bax C-terminus, preventing Bax activation and activation of the intrinsic mitochondrial pathway PMID: 15383280
    28. ARC is recruited to the Fas DISC. By interacting with Fas and FADD through CARD-DD and CARD-DED interactions, ARC prevents DISC assembly and procaspase-8 activation. PMID: 15383280

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  • 相关疾病：
    Myoclonus, familial cortical (FCM)
  • 亚细胞定位：
    [Isoform 1]: Nucleus, nucleolus.; [Isoform 3]: Cytoplasm.; [Isoform 2]: Cytoplasm. Mitochondrion. Sarcoplasmic reticulum. Membrane; Lipid-anchor.
  • 组织特异性：
    Highly expressed in heart and skeletal muscle. Detected at low levels in placenta, liver, kidney and pancreas.
  • 数据库链接：

    HGNC: 7869

    OMIM: 605235

    KEGG: hsa:8996

    STRING: 9606.ENSP00000268605

    UniGene: Hs.513667