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  4. Recombinant Human PDZ and LIM domain protein 4 (PDLIM4)

Recombinant Human PDZ and LIM domain protein 4 (PDLIM4)

  • 中文名称：
    人PDLIM4重组蛋白
  • 货号：
    CSB-YP017735HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人PDLIM4重组蛋白
  • 货号：
    CSB-EP017735HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人PDLIM4重组蛋白
  • 货号：
    CSB-EP017735HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人PDLIM4重组蛋白
  • 货号：
    CSB-BP017735HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人PDLIM4重组蛋白
  • 货号：
    CSB-MP017735HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    PDLIM4
  • Uniprot No.：
  • 别名：
    Enigma homolog; LIM domain protein; LIM domain protein RIL; LIM protein RIL; PDLI4_HUMAN; PDLIM 4; PDLIM4; PDZ and LIM domain 4; PDZ and LIM domain protein 4; Reversion induced LIM protein; Reversion-induced LIM protein
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-330
  • 氨基酸序列
    MPHSVTLRGP SPWGFRLVGG RDFSAPLTIS RVHAGSKAAL AALCPGDLIQ AINGESTELM THLEAQNRIK GCHDHLTLSV SRPEGRSWPS APDDSKAQAH RIHIDPEIQD GSPTTSRRPS GTGTGPEDGR PSLGSPYGQP PRFPVPHNGS SEATLPAQMS TLHVSPPPSA DPARGLPRSR DCRVDLGSEV YRMLREPAEP VAAEPKQSGS FRYLQGMLEA GEGGDWPGPG GPRNLKPTAS KLGAPLSGLQ GLPECTRCGH GIVGTIVKAR DKLYHPECFM CSDCGLNLKQ RGYFFLDERL YCESHAKARV KPPEGYDVVA VYPNAKVELV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Suppresses SRC activation by recognizing and binding to active SRC and facilitating PTPN13-mediated dephosphorylation of SRC 'Tyr-419' leading to its inactivation. Inactivated SRC dissociates from this protein allowing the initiation of a new SRC inactivation cycle. Involved in reorganization of the actin cytoskeleton. In nonmuscle cells, binds to ACTN1 (alpha-actinin-1), increases the affinity of ACTN1 to F-actin (filamentous actin), and promotes formation of actin stress fibers. Involved in regulation of the synaptic AMPA receptor transport in dendritic spines of hippocampal pyramidal neurons directing the receptors toward an insertion at the postsynaptic membrane. Links endosomal surface-internalized GRIA1-containing AMPA receptors to the alpha-actinin/actin cytoskeleton. Increases AMPA receptor-mediated excitatory postsynaptic currents in neurons.; Involved in reorganization of the actin cytoskeleton and in regulation of cell migration. In response to oxidative stress, binds to NQO1, which stabilizes it and protects it from ubiquitin-independent degradation by the core 20S proteasome. Stabilized protein is able to heterodimerize with isoform 1 changing the subcellular location of it from cytoskeleton and nuclei to cytosol, leading to loss of isoforms 1 ability to induce formation of actin stress fibers. Counteracts the effects produced by isoform 1 on organization of actin cytoskeleton and cell motility to fine-tune actin cytoskeleton rearrangement and to attenuate cell migration.
  • 基因功能参考文献：
    1. Methylation of HIN-1, RASSF1A, RIL and CDH13 in breast cancers was associated with clinical characteristics, but only RASSF1A methylation was associated with time to first recurrence and overall survival. PMID: 22695491
    2. Actin cytoskeleton remodeling by the alternatively spliced isoform of PDLIM4/RIL protein. PMID: 21636573
    3. RIL and its variant sequences may be an important determinant of osteoporosis PMID: 12908099
    4. Ril modulates actin stress fiber turnover and enhances the association with actinin and actins. PMID: 14729062
    5. Results showed that the hypermethylation of PDLIM4 gene could be used as a sensitive molecular tool in detection of prostate. PMID: 16489065
    6. RIL methylation is a marker of adverse prognosis independent of chromosome 5 and 7 deletions. PMID: 17332327
    7. several genes that are known to be regulated by DNA methylation were up-regulated dramatically by integrin alpha6beta4 expression, including S100A4, FST, PDLIM4, CAPG, and Nkx2.2. PMID: 19011242
    8. PDLIM4 may function as a tumor suppressor, involved in the control of cell proliferation by associating with actin in prostate cancer cells. PMID: 19212833
    9. Reversion-induced LIM interaction with Src reveals a novel Src inactivation cycle. PMID: 19307596

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  • 亚细胞定位：
    [Isoform 1]: Cytoplasm, cytoskeleton. Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Cell projection, lamellipodium. Cell projection, dendritic spine. Early endosome membrane; Peripheral membrane protein; Cytoplasmic side. Recycling endosome membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, synapse, synaptosome.; [Isoform 2]: Cytoplasm.
  • 组织特异性：
    [Isoform 2]: Found in brain.
  • 数据库链接：

    HGNC: 16501

    OMIM: 603422

    KEGG: hsa:8572

    STRING: 9606.ENSP00000253754

    UniGene: Hs.424312