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  4. Recombinant Human PR domain zinc finger protein 14 (PRDM14)

Recombinant Human PR domain zinc finger protein 14 (PRDM14)

  • 货号：
    CSB-YP863934HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP863934HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP863934HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP863934HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP863934HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    PRDM14
  • Uniprot No.：
  • 别名：
    MGC59730; PFM 11; PFM11; PR domain containing 14; PR domain zinc finger protein 14; PR domain-containing protein 14; PRD14_HUMAN; PRDM 14; PRDM14
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-571
  • 氨基酸序列
    MALPRPSEAV PQDKVCYPPE SSPQNLAAYY TPFPSYGHYR NSLATVEEDF QPFRQLEAAA SAAPAMPPFP FRMAPPLLSP GLGLQREPLY DLPWYSKLPP WYPIPHVPRE VPPFLSSSHE YAGASSEDLG HQIIGGDNES GPCCGPDTLI PPPPADASLL PEGLRTSQLL PCSPSKQSED GPKPSNQEGK SPARFQFTEE DLHFVLYGVT PSLEHPASLH HAISGLLVPP DSSGSDSLPQ TLDKDSLQLP EGLCLMQTVF GEVPHFGVFC SSFIAKGVRF GPFQGKVVNA SEVKTYGDNS VMWEIFEDGH LSHFIDGKGG TGNWMSYVNC ARFPKEQNLV AVQCQGHIFY ESCKEIHQNQ ELLVWYGDCY EKFLDIPVSL QVTEPGKQPS GPSEESAEGY RCERCGKVFT YKYYRDKHLK YTPCVDKGDR KFPCSLCKRS FEKRDRLRIH ILHVHEKHRP HKCSTCGKCF SQSSSLNKHM RVHSGDRPYQ CVYCTKRFTA SSILRTHIRQ HSGEKPFKCK YCGKSFASHA AHDSHVRRSH KEDDGCSCSI CGKIFSDQET FYSHMKFHED Y
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcription factor that has both positive and negative roles on transcription. Required for the maintenance of embryonic stem cell identity and the reacquisition of pluripotency in somatic cells. May play an essential role in germ cell development at 2 levels: the reacquisition of potential pluripotency, including SOX2 up-regulation, and successful epigenetic reprogramming, characterized by EHMT1 repression. Its association with CBFA2T2 is required for the functions in pluripotency and germ cell formation. Directly up-regulates the expression of pluripotency gene POU5F1 through its proximal enhancer. Binds to the DNA consensus sequence 5'-GGTC[TC]CTAA-3'.
  • 基因功能参考文献：
    1. Our results suggest that PRDM14 serves as an initial trigger for epigenetic changes and hyperdynamic plasticity in tumors via bivalent chromatin domains. PMID: 28423353
    2. Results identified GRP78 and HSP90a as binding partners of PRDM14 in triple-negative breast cancer cells, and all participate in cancer regulation. The interactions were direct and required the C-terminal region including the zinc finger motifs of PRDM14. PMID: 29178343
    3. miR-424-->cdc42-->prdm14 axis as a key molecular signalling cascade that might influence breast cancer progression in diabetic patients through hyperactivation of cancer stem cells. PMID: 29024936
    4. High expression of PRDM14 is associated with liver metastasis in pancreatic cancer. PMID: 28498896
    5. PRDM14 role in cell proliferation and cell migration PMID: 27693212
    6. PRDM14 bears a single PR domain and six tandemly repeated zinc fi ngers, which is involved in the process of the deacetylation and methylation of the histone, and is involved in the formation of tumor . [review] PMID: 26903163
    7. In response to cytokines, pluripotent stem cells differentiate first into a heterogeneous mesoderm-like cell population and then into Primordial Germ cell-Like Cells, which exhibit minimal PRDM14 expression. PMID: 25750208
    8. The contribution of methylation-mediated gene silencing of PRDM14 to apoptosis evasion in HPV-positive cancer cells offers novel therapeutic options for HPV-induced cancers. PMID: 25233927
    9. PRDM14 associated with cell differentiation in NSCLC. PMID: 23690269
    10. Study reveals a repressive role of PRDM14 in the maintenance and induction of pluripotency and identifies PRDM14 as a new regulator of PRC2. PMID: 23280602
    11. The high expression of PRDM14 in non-small cell lung cancer is associated with differentiation and histological type. PMID: 20840815
    12. novel hESC regulators wherein PRDM14 exemplifies a key transcription factor required for the maintenance of hESC identity and the reacquisition of pluripotency in human somatic cells PMID: 20953172
    13. PRDM14 mRNA is overexpressed in about two thirds of breast cancers; moreover, immunohistochemical analysis showed that expression of PRDM14 protein is also up-regulated. PMID: 17942894
    14. These results suggest that PRDM14 is involved in the maintenance of the self-renewal of human ES cells by suppression of gene expression. PMID: 18194669

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Class V-like SAM-binding methyltransferase superfamily
  • 组织特异性：
    Expressed in embryonic stem cells. Tends to be overexpressed in breast cancer (at protein level).
  • 数据库链接：

    HGNC: 14001

    OMIM: 611781

    KEGG: hsa:63978

    STRING: 9606.ENSP00000276594

    UniGene: Hs.736037