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  4. Recombinant Human Pancreas/duodenum homeobox protein 1 (PDX1)

Recombinant Human Pancreas/duodenum homeobox protein 1 (PDX1)

  • 中文名称：
    人PDX1重组蛋白
  • 货号：
    CSB-YP017746HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人PDX1重组蛋白
  • 货号：
    CSB-EP017746HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人PDX1重组蛋白
  • 货号：
    CSB-EP017746HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人PDX1重组蛋白
  • 货号：
    CSB-BP017746HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人PDX1重组蛋白
  • 货号：
    CSB-MP017746HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    PDX1
  • Uniprot No.：
  • 别名：
    Glucose sensitive factor; Glucose-sensitive factor; GSF; IDX 1; IDX-1; IDX1; Insulin promoter factor 1; Insulin promoter factor 1 homeodomain transcription factor; Insulin upstream factor 1; IPF 1; IPF-1; IPF1 ; Islet/duodenum homeobox 1; Islet/duodenum homeobox-1; IUF 1; IUF-1; IUF1; MODY4; Pancreas/duodenum homeobox 1; Pancreas/duodenum homeobox protein 1; pancreatic and duodenal homeobox P; PDX 1; PDX-1; PDX1; PDX1_HUMAN; Somatostatin transactivating factor 1; Somatostatin-transactivating factor 1; STF 1; STF-1; STF1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-283
  • 氨基酸序列
    MNGEEQYYAA TQLYKDPCAF QRGPAPEFSA SPPACLYMGR QPPPPPPHPF PGALGALEQG SPPDISPYEV PPLADDPAVA HLHHHLPAQL ALPHPPAGPF PEGAEPGVLE EPNRVQLPFP WMKSTKAHAW KGQWAGGAYA AEPEENKRTR TAYTRAQLLE LEKEFLFNKY ISRPRRVELA VMLNLTERHI KIWFQNRRMK WKKEEDKKRG GGTAVGGGGV AEPEQDCAVT SGEELLALPP PPPPGGAVPP AAPVAAREGR LPPGLSASPQ PSSVAPRRPQ EPR
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Activates insulin, somatostatin, glucokinase, islet amyloid polypeptide and glucose transporter type 2 gene transcription. Particularly involved in glucose-dependent regulation of insulin gene transcription. As part of a PDX1:PBX1b:MEIS2b complex in pancreatic acinar cells is involved in the transcriptional activation of the ELA1 enhancer; the complex binds to the enhancer B element and cooperates with the transcription factor 1 complex (PTF1) bound to the enhancer A element. Binds preferentially the DNA motif 5'-[CT]TAAT[TG]-3'. During development, specifies the early pancreatic epithelium, permitting its proliferation, branching and subsequent differentiation. At adult stage, required for maintaining the hormone-producing phenotype of the beta-cell.
  • 基因功能参考文献：
    1. heat shock proteins are involved in PDX1-mediated effects on the genesis of gastric cancer PMID: 30310259
    2. Investigated the effect of gestational diabetes mellitus (GDM) on the expression and methylation of PGC-1alpha and PDX1 in placenta and their effects on fetal glucose metabolism. Blood glucose was negatively correlated with the expression of PGC-1alpha and PDX1 mRNA in the placenta. PMID: 29730292
    3. PDX1, Neurogenin-3, and MAFA are critical transcription regulators for beta cell development and regeneration. (Review) PMID: 29096722
    4. study provides a novel culture protocol for generating pancreas-committed cells from hAECs and reveals an interplay between Pdx1 and activin A/nicotinamide signaling in early pancreatic fate determination. PMID: 28632450
    5. Data indicate the secretory granule membrane glycoprotein 2 as a marker for PDX1+/NKX6-1+ pancreatic progenitors (PPs). PMID: 28835709
    6. expression of regulatory master genes of embryonic development in pancreatic cancer cells on the intracellular concentration of the master regulator PDX1 PMID: 28864896
    7. Efficiency of differentiation of Induced pluripotent stem cells to insulin producing cells can be increased by concurrent expression of PDX1 and NKX6.1 during progenitor cells maturation. PMID: 27998294
    8. Mutation in PDX1 gene is associated with Maturity Onset Diabetes of the Young. PMID: 28095440
    9. Results found that Pdx-1 overexpression exerts its effects on islet cell replication via a noncell-autonomous mechanism involving induction of a secreted soluble factor or factors. Also, Inhbb is identified as one factor mediating the proliferative effect of Pdx-1. PMID: 27620967
    10. Our findings indicate that MET induces the differentiation of Pdx1-expressing cells within the definitive endoderm in a time-dependent manner, and suggest useful application for regenerative medicine. PMID: 28202025
    11. Pdx1 and MafA play crucial roles in the pancreas and maintain mature beta-cell function. Our results showed that the expression of Pdx1 and MafA were significantly upregulated after a sleeve gastrectomy for morbid obesity. PMID: 26781599
    12. Data show that the expression patterns of sex determining region Y-box 2 (SOX2) and pancreatic and duodenal homeobox 1 (Pdx1)) highly correlated with prognosis of pancreatic neuroendocrine tumors (p-NETs). PMID: 26491904
    13. The ERK-PDX-1 interaction in PANC-1 cells. PMID: 26695692
    14. Foxo1 and PDX1 are colocalized in fetal pancreatic islets, suggesting they function in parallel in beta cells. PMID: 26727107
    15. This study investigated the role of Pdx1 and its mechanism in differentiating induced pluripotent stem cells (iPSCs) into islet beta cells. PMID: 26345820
    16. the human Pdx1 is highly effective in triggering human biliary tree stem cells differentiation toward functional beta-pancreatic cells. PMID: 26252949
    17. Introduction of the PDX1 gene makes cells acquire a functional insulin secretory capacity PMID: 25817342
    18. These results suggest that IFN-gamma induced beta-cell dysfunction is mediated through decreased nuclear localization of Pdx-1. PMID: 25839229
    19. The rs12255372 SNP of TCF7L2 and D76N of PDX-1 genes may confer susceptibility to T2DM in the population living in Mashhad, Iran. PMID: 26058934
    20. Pdx1 has a role in pancreatic differentiation in human amniotic fluid-derived stem cells PMID: 23147868
    21. physiological and pathophysiological conditions dynamically regulated Pdx1 binding to these Swi/Snf complexes in vivo PMID: 25801033
    22. we found that PDX1 binds and suppresses expression of endogenous liver genes. These findings rebrand PDX1 as a context-dependent transcriptional repressor and activator within the same cell type. PMID: 25843046
    23. PDX-1mRNA is detectable in samples of pancreatic cancer. Its quantification may be helpful to improve the diagnosis of pancreatic cancer. PMID: 25454709
    24. These results indicate that PDX-1 mRNA may offer a promising approach to produce safe IPCs for clinical diabetes mellitus treatment. PMID: 25201603
    25. CONCLUSIONS: Our results demonstrate the use of in vitro-synthesized PDX1 messenger RNA to differentiate hUC-MSCs into islet-like cells and pave the way toward the development of reprogramming and directed-differentiation methods PMID: 25287601
    26. MAFA, MAFB, NKX6.1, and PDX1 activity provides a gauge of islet beta cell function, with loss of MAFA (and/or MAFB) representing an early indicator of beta cell inactivity PMID: 23863625
    27. A rare (0.20%) frameshift variant in PDX1, encoding p.Gly218Alafs*12, associates with high risk of type 2 diabetes (OR = 2.27, P = 7.3 x 10(-7)). PMID: 24464100
    28. Performed fully atomistic Molecular Dynamics simulations both in crystal and aqueous environments in order to elucidate the nature of the difference in binding contacts between Pdx1 and DNA (PDB 2H1K) . PMID: 23950697
    29. Developed a liquid-phase, non-radioactive, sensitive and specific luciferase immunoprecipitation systems assay to detect pdx1 autoantibodies in human sera. PMID: 23696946
    30. Pdx1 is a transcription factor required for the formation of endocrine pancreas that is inhibited during Coxsackievirus B4 infection of human pancreas ductal cells PMID: 23775130
    31. recessive PDX1 mutations are a rare but important cause of isolated permanent neonatal diabetes in patients without pancreatic hypoplasia/agenesis PMID: 23320570
    32. PASK phosphorylates and inactivates GSK3beta, thereby preventing PDX-1 serine phosphorylation and alleviating GSK3beta-mediated PDX-1 protein degradation in pancreatic beta-cells. PMID: 23853095
    33. studies indicated p38 MAP kinase is a positive regulator of PDX-1 stability and p38 exerts its stabilizing effect on PDX-1 through a phosphorylation-dependent inhibition of PDX-1 ubiquitination. PMID: 23331010
    34. PDX-1 is a therapeutic target for both hormonal symptoms and tumor volume in models of pancreatic cancer, insulinoma and islet neoplasia PMID: 22905092
    35. Up-regulation of Pdx1 is a feature of cholangiocarcinogenesis associated with chronic cholangitis. PMID: 22594685
    36. data demonstrate epigenetic modifications of PDX-1 may reduce its expression in diabetic islets which may lead to impaired insulin expression and secretion; data further suggest hyperglycemia may be a factor behind increased DNA methylation and decreased expression of PDX-1 PMID: 22570331
    37. Combined transfection of the three transcriptional factors, PDX-1, NeuroD1, and MafA, causes differentiation of bone marrow mesenchymal stem cells into insulin-producing cells PMID: 22761608
    38. PDX1 repression of FABP1, LCT, and SI suggests a role for PDX1 in patterning anterior intestinal development. PMID: 22640736
    39. Analysis of cell death genes led to the identification of Noxa and Puma as direct targets of Pdx1 PMID: 22322596
    40. Through multiple mechanisms, SREBP-1c, when induced in a lipotoxic state, repressed PDX-1 expression contributing to the inhibition of insulin expression and beta cell dysfunction. PMID: 21652712
    41. a significant relation of PDX1 and CDX2 expressions among phenotypic classification of gastric carcinomas reveals an idea about similar functions for PDX1 and CDX2 in the evolution of gastric carcinoma. PMID: 21909647
    42. PDX-1 plays an important role in the induction of CD44+/CD105+ human amniotic fluid cells (HuAFCs) into functional pancreatic beta-cell-like cells in vitro. PMID: 21612404
    43. Gastrin-positive neuroendocrine tumors, whether of duodenal or pancreatic origin, frequently expressed PDX1 (17/18), ISL1 (14/18), and NGN3 (14/18). PMID: 21739268
    44. PDX1, important for the regulation of insulin metabolism regulation, was able to repress the TBX15 promoter in a methylation-dependent manner, which might, at least partially, explain the specific mRNA decrease of TBX15 observed in vIUGR placentas. PMID: 20962579
    45. ATF2 interacts with beta-cell-enriched transcription factors, MafA, Pdx1, and beta2, and activates insulin gene transcription. PMID: 21278380
    46. pdx-1 functions as a "pancreas-permitting gene". PMID: 20668890
    47. Data suggest that NKX6.1 activates immature pancreatic markers but not pancreatic hormone gene expression in human liver cells, and suggest a potential role for NKX6.1 in promoting PDX-1 reprogrammed maturation along a beta-cell-like lineage. PMID: 21108535
    48. TFF1, TFF2, and PDX1 were expressed only in lobular endocervical glandular hyperplasia. PMID: 21228366
    49. Results indicate that beta-cell dysfunction during progression of CP correlates with the decrease in PDX-1 gene expression. PMID: 20467340
    50. in adult mouse beta cells, Pcif1 limits Pdx1 protein accumulation and thus the expression of insulin and other gene targets important in the maintenance of beta cell mass and function. PMID: 20811152

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  • 相关疾病：
    Pancreatic agenesis 1 (PAGEN1); Diabetes mellitus, non-insulin-dependent (NIDDM); Maturity-onset diabetes of the young 4 (MODY4)
  • 亚细胞定位：
    Nucleus. Cytoplasm, cytosol.
  • 蛋白家族：
    Antp homeobox family, IPF1/XlHbox-8 subfamily
  • 组织特异性：
    Duodenum and pancreas (Langerhans islet beta cells and small subsets of endocrine non-beta-cells, at low levels in acinar cells).
  • 数据库链接：

    HGNC: 6107

    OMIM: 125853

    KEGG: hsa:3651

    STRING: 9606.ENSP00000370421

    UniGene: Hs.32938