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  4. Recombinant Human Pancreas transcription factor 1 subunit alpha (PTF1A)

Recombinant Human Pancreas transcription factor 1 subunit alpha (PTF1A)

  • 货号：
    CSB-YP752531HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP752531HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP752531HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP752531HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP752531HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    PTF1A
  • Uniprot No.：
  • 别名：
    bHLH transcription factor p48; bHLHa29; Class A basic helix-loop-helix protein 29; Class II bHLH protein PTF1A; Exocrine pancreas specific transcription factor p48; p48 DNA binding subunit of transcription factor PTF1; p48 DNA-binding subunit of transcription factor PTF1; PACA; PAGEN2; Pancreas specific transcription factor 1a; Pancreas transcription factor 1 subunit alpha; Pancreas-specific transcription factor 1a; PTF 1A; PTF1 p48; PTF1-p48; Ptf1a; PTF1A_HUMAN; PTF1P48
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-328
  • 氨基酸序列
    MDAVLLEHFP GGLDAFPSSY FDEDDFFTDQ SSRDPLEDGD ELLADEQAEV EFLSHQLHEY CYRDGACLLL QPAPPAAPLA LAPPSSGGLG EPDDGGGGGY CCETGAPPGG FPYSPGSPPS CLAYPCAGAA VLSPGARLRG LSGAAAAAAR RRRRVRSEAE LQQLRQAANV RERRRMQSIN DAFEGLRSHI PTLPYEKRLS KVDTLRLAIG YINFLSELVQ ADLPLRGGGA GGCGGPGGGG RLGGDSPGSQ AQKVIICHRG TRSPSPSDPD YGLPPLAGHS LSWTDEKQLK EQNIIRTAKV WTPEDPRKLN SKSSFNNIEN EPPFEFVS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcription factor implicated in the cell fate determination in various organs. Binds to the E-box consensus sequence 5'-CANNTG-3'. Plays a role in early and late pancreas development and differentiation. Important for determining whether cells allocated to the pancreatic buds continue towards pancreatic organogenesis or revert back to duodenal fates. May be involved in the maintenance of exocrine pancreas-specific gene expression including ELA1 and amylase. Required for the formation of pancreatic acinar and ductal cells. Plays an important role in cerebellar development. Directly regulated by FOXN4 and RORC during retinal development, FOXN4-PTF1A pathway plays a central role in directing the differentiation of retinal progenitors towards horizontal and amacrine fates.
  • 基因功能参考文献：
    1. non-neural progenitor transcription factor Ptf1a alone is sufficient to directly reprogram mouse and human fibroblasts into self-renewable induced neural stem cells capable of differentiating into functional neurons, astrocytes and oligodendrocytes, and improving cognitive dysfunction of Alzheimer's disease mouse models when transplanted PMID: 30030434
    2. Study describes the wide range of gene control by PTF1A that maintains the specific characteristics of pancreatic acinar cell identity and differentiation. PTF1A controls the pancreatic acinar transcription program by direct action at a thousand genes and in collaboration with other less cell type-restricted factors to ensure acinar cell homeostasis and to suppress other cell-type-specific programs. PMID: 27697859
    3. Data show isolated Pancreatic Aplasia Due to a Hypomorphic PTF1A Mutation PMID: 27284104
    4. The results of this study suggested Ptf1a-dependent cell-fate misspecification as a novel mechanism of human brainstem pathology. PMID: 26937009
    5. PTF1A enhancer mutations have been shown to cause neonatal diabetes associated with pancreatic agenesis PMID: 26184423
    6. Loss of Ptf1a alone is sufficient to induce acinar-to-ductal metaplasia, potentiate inflammation, and induce a KRAS-permissive, pancreatic ductal adenocarcinoma-like gene expression profile. PMID: 26151762
    7. The results of the present study do not support the hypothesis that high penetrant mutations in these regions of PTF1A are involved in the development of human VATER/VACTERL association or NTDs PMID: 25775927
    8. ata indicate that E3 ubiquitin ligase thyroid hormone receptor-interacting protein 12 (TRIP12) promotes proteasomal degradation of pancreas transcription factor 1a (PTF1a)and regulates PTF1a activities. PMID: 25355311
    9. Recessive mutations in a distal PTF1A enhancer cause isolated pancreatic agenesis. PMID: 24212882
    10. ICAT is a novel Ptf1a interactor that regulates pancreatic acinar differentiation and displays altered expression. PMID: 23339455
    11. ptf1a functions as a "pancreatic determiner". PMID: 20668890
    12. P48 interacts with the RBP-L and RBP-J subunits primarily through two short conserved tryptophan-containing motifs, similar to the motif of the Notch intracellular domain (NotchIC) that interacts with RBP-J. PMID: 16354684
    13. Controls the fate, survival, differentiation, and migration of climbing fiber neurons during their development in the transgenic murine inferior olivary nucleus. PMID: 17928434
    14. A critical and selective role of p/CAF in PTF1-dependent gene activation during acinar differentiation. PMID: 18834332
    15. Here we report a new case of cerebellar agenesis and neonatal diabetes mellitus whose parents are PTF1A mutation carriers. PMID: 19650412
    16. Two separable genomic regions code for initiation and positive feedback of Ptf1a transgene expression in the developing nervous system. PMID: 19741120

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  • 相关疾病：
    Pancreatic and cerebellar agenesis (PACA); Pancreatic agenesis 2 (PAGEN2)
  • 亚细胞定位：
    Nucleus. Cytoplasm.
  • 组织特异性：
    Pancreas-specific (at protein level). Loss of expression is seen in ductal type pancreas cancers.
  • 数据库链接：

    HGNC: 23734

    OMIM: 607194

    KEGG: hsa:256297

    STRING: 9606.ENSP00000365687

    UniGene: Hs.351503