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  4. Recombinant Human Phosphatidylinositol 5-phosphate 4-kinase type-2 alpha (PIP4K2A)

Recombinant Human Phosphatidylinositol 5-phosphate 4-kinase type-2 alpha (PIP4K2A)

  • 中文名称：
    人PIP4K2A重组蛋白
  • 货号：
    CSB-YP018022HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人PIP4K2A重组蛋白
  • 货号：
    CSB-EP018022HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人PIP4K2A重组蛋白
  • 货号：
    CSB-EP018022HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人PIP4K2A重组蛋白
  • 货号：
    CSB-BP018022HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人PIP4K2A重组蛋白
  • 货号：
    CSB-MP018022HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    1 phosphatidylinositol 4 phosphate 5 kinase 2 alpha ; 1 phosphatidylinositol 4 phosphate 5 kinase; 1 phosphatidylinositol 4 phosphate kinase; 1-phosphatidylinositol-5-phosphate 4-kinase 2-alpha; 4 5 kinase B isoform; 4 5 kinase C isoform; 4 5 kinase isoform 2 alpha; Diphosphoinositide kinase 2 alpha; Diphosphoinositide kinase 2-alpha; FLJ13267; Phosphatidylinositol 4 phosphate 5 kinase type 2 alpha; Phosphatidylinositol 4 phosphate 5 kinase type II alpha; Phosphatidylinositol-5-phosphate 4-kinase type II alpha; Phosphatidylinositol-5-phosphate 4-kinase type-2 alpha; PI(5)P 4-kinase type II alpha; PI42A_HUMAN; PI5P4KA; PIP4K2A; PIP4KII-alpha; PIP5K2 ; PIP5K2 alpha; PIP5K2A; PIP5KII alpha; PIP5KII-alpha; PIP5KIIA; PIP5KIII; PIPK ; PtdIns ; PtdIns(4)P-5-kinase B isoform; PtdIns(4)P-5-kinase C isoform; PtdIns(5)P-4-kinase isoform 2-alpha; Type II phosphatidylinositol 4 phosphate 5 kinase 53 K isoform
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-406
  • 氨基酸序列
    ATPGNLGSS VLASKTKTKK KHFVAQKVKL FRASDPLLSV LMWGVNHSIN ELSHVQIPVM LMPDDFKAYS KIKVDNHLFN KENMPSHFKF KEYCPMVFRN LRERFGIDDQ DFQNSLTRSA PLPNDSQARS GARFHTSYDK RYIIKTITSE DVAEMHNILK KYHQYIVECH GITLLPQFLG MYRLNVDGVE IYVIVTRNVF SHRLSVYRKY DLKGSTVARE ASDKEKAKEL PTLKDNDFIN EGQKIYIDDN NKKVFLEKLK KDVEFLAQLK LMDYSLLVGI HDVERAEQEE VECEENDGEE EGESDGTHPV GTPPDSPGNT LNSSPPLAPG EFDPNIDVYG IKCHENSPRK EVYFMAIIDI LTHYDAKKKA AHAAKTVKHG AGAEISTVNP EQYSKRFLDF IGHILT
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the phosphorylation of phosphatidylinositol 5-phosphate (PtdIns5P) on the fourth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). Has both ATP- and GTP-dependent kinase activities. May exert its function by regulating the levels of PtdIns5P, which functions in the cytosol by increasing AKT activity and in the nucleus signals through ING2. May regulate the pool of cytosolic PtdIns5P in response to the activation of tyrosine phosphorylation. Required for lysosome-peroxisome membrane contacts and intracellular cholesterol transport through modulating peroxisomal PtdIns(4,5)P2 level. In collaboration with PIP4K2B, has a role in mediating autophagy in times of nutrient stress. Required for autophagosome-lysosome fusion and the regulation of cellular lipid metabolism. May be involved in thrombopoiesis, and the terminal maturation of megakaryocytes and regulation of their size. Negatively regulates insulin signaling through a catalytic-independent mechanism. PIP4Ks interact with PIP5Ks and suppress PIP5K-mediated PtdIns(4,5)P2 synthesis and insulin-dependent conversion to PtdIns(3,4,5)P3.
  • 基因功能参考文献：
    1. Recombinant PIP4K2A has the RNA binding activity and can associate specifically with Plasmodium 3'UTR RNAs. PMID: 29518392
    2. the rs7088318 (PIP4K2A) and rs2239633 (CEBPE) polymorphisms were not associated with ALL risk. PMID: 28476190
    3. meta-analysis of association of SNPs rs7088318 and rs4748793 and acute lymphoblastic leukemia susceptibility PMID: 27149463
    4. PIP5K2A is possibly involved in a mechanism protecting against tardive dyskinesia-inducing neurotoxicity. PMID: 25548108
    5. The diplotype ATTGCT/ATTGCT of the PIP4K2A gene confers approximately three-times higher incomplete responsiveness towards antipsychotics in severely ill patients. PMID: 25025909
    6. PIP4K2A to be essential for the clonogenic and leukemia-initiating potential of human AML cells, and for the clonogenic potential of murine MLL-AF9 AML cells. PMID: 24681948
    7. We also showed that PIPKIIalpha silencing can induce alpha and gamma globin expression and decrease cell proliferation in K562 cells. PMID: 24788727
    8. Overexpression of type 2A PI(4)P5-kinase (PIP5K 2A) significantly reduced tamoxifen inhibition of Kv7.2/Kv7.3 and Kv7.2 R463Q channels. PMID: 24086693
    9. The data suggest that PI5P4Ka N251S does not significantly differ in activity from the wild-type enzyme, throwing a new light on its association with schizophrenia. PMID: 24081551
    10. Knocking down PI5P4Kalpha and beta in a breast cancer cell line bearing an amplification of the gene encoding PI5P4K beta and deficient for p53 impaired growth on plastic and in xenografts. PMID: 24209622
    11. Variation at 10p12.2 (PIP4K2A) and 10p14 (GATA3) influences risk of acute lymphoblastic anemia and tumor subtype. (Meta-analysis) PMID: 23996088
    12. PIP5K2A variant influences susceptibility to schizophrenia in the Russian population of Siberia. s studied 355 patients with schizophrenic disorders from the Russian population of Siberia. PMID: 23739505
    13. The present study demonstrated that expression of PIP4K2A mRNA is significantly increased in LCL derived from patients with schizophrenia PMID: 21377334
    14. Studies indicate that the gene PIP5K2A was significantly elevated in stroma cells in distant metastasis group, compared to stroma in no distant metastasis group. PMID: 21124964
    15. PIP4Kbeta interacts with and modulates nuclear localization of the PIP4Kalpha. PMID: 20583997
    16. A homogeneous sample of 280 schizophrenia patients and 230 healthy controls of Hungarian, Caucasian descent were genotyped for polymorphisms in schizophrenia candidate genes NRG1, DTNBP1, RGS4, G72/G30, and PIP5K2A. PMID: 19937977
    17. study supports a minor role for PIP4K2A in schizophrenia etiology in the Irish population PMID: 19475563
    18. PIP5K2A is a novel signaling element in the regulation of EAAT3 activity PMID: 19644675
    19. translocation by protein kinase C is required for platelet alpha-granule secretion PMID: 12509423
    20. Single nucleotide polymorphism is associated with schizophfenia. PMID: 16801950
    21. The strongest evidence for schizophrenia association was found for the A-allele of SNP rs10828317 in the PIP5K2A gene, which was associated with both clinical subtypes. This SNP leads to a change in protein composition. PMID: 17410640
    22. PIP5K2A gene is implicated in schizophrenia in Chinese population PMID: 17555944
    23. rs11013052 was significantly associated with schizophrenia in this Indonesian family sample PMID: 18314871
    24. unlike PtdIns5P production enhanced by cell stress, we show that this pool of PtdIns5P is specifically regulated by the inositol lipid kinase PIP4K2a PMID: 18364242
    25. We find that wild-type PIP5K2A but not the schizophrenia-associated mutant (N251S)-PIP5K2A activates heteromeric KCNQ2/KCNQ3 and KCNQ3/KCNQ5, the molecular correlate of neuronal M channels. PMID: 18545987
    26. PIP4KIIA may be one of the factors related to the regulation of the beta-globin gene expression and the different levels of Hb H in alpha-thalassemic patients PMID: 19656170

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  • 亚细胞定位：
    Cell membrane. Nucleus. Lysosome. Cytoplasm. Photoreceptor inner segment. Cell projection, cilium, photoreceptor outer segment.
  • 组织特异性：
    Expressed ubiquitously, with high levels in the brain. Present in most tissues, except notably skeletal muscle and small intestine.
  • 数据库链接：

    HGNC: 8997

    OMIM: 603140

    KEGG: hsa:5305

    STRING: 9606.ENSP00000365757

    UniGene: Hs.57079