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  4. Recombinant Human Pleckstrin homology domain-containing family O member 1 (PLEKHO1)

Recombinant Human Pleckstrin homology domain-containing family O member 1 (PLEKHO1)

  • 货号：
    CSB-YP706633HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP706633HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP706633HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP706633HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP706633HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    PLEKHO1
  • Uniprot No.：
  • 别名：
    C-Jun-binding protein; Casein kinase 2-interacting protein 1; CK2 interacting protein 1; CK2 interacting protein 1 HQ0024c protein; CK2-interacting protein 1; CKIP 1; CKIP-1; CKIP1; JBP; OC120; Osteoclast maturation associated gene 120 protein; Osteoclast maturation-associated gene 120 protein; PH domain-containing family O member 1; PKHO1_HUMAN; Pleckstrin homology domain containing family O member 1; Pleckstrin homology domain-containing family O member 1; PLEKHO1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-409
  • 氨基酸序列
    MMKKNNSAKR GPQDGNQQPA PPEKVGWVRK FCGKGIFREI WKNRYVVLKG DQLYISEKEV KDEKNIQEVF DLSDYEKCEE LRKSKSRSKK NHSKFTLAHS KQPGNTAPNL IFLAVSPEEK ESWINALNSA ITRAKNRILD EVTVEEDSYL AHPTRDRAKI QHSRRPPTRG HLMAVASTST SDGMLTLDLI QEEDPSPEEP TSCAESFRVD LDKSVAQLAG SRRRADSDRI QPSADRASSL SRPWEKTDKG ATYTPQAPKK LTPTEKGRCA SLEEILSQRD AASARTLQLR AEEPPTPALP NPGQLSRIQD LVARKLEETQ ELLAEVQGLG DGKRKAKDPP RSPPDSESEQ LLLETERLLG EASSNWSQAK RVLQEVRELR DLYRQMDLQT PDSHLRQTTP HSQYRKSLM
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Plays a role in the regulation of the actin cytoskeleton through its interactions with actin capping protein (CP). May function to target CK2 to the plasma membrane thereby serving as an adapter to facilitate the phosphorylation of CP by protein kinase 2 (CK2). Appears to target ATM to the plasma membrane. Appears to also inhibit tumor cell growth by inhibiting AKT-mediated cell-survival. Also implicated in PI3K-regulated muscle differentiation, the regulation of AP-1 activity (plasma membrane bound AP-1 regulator that translocates to the nucleus) and the promotion of apoptosis induced by tumor necrosis factor TNF. When bound to PKB, it inhibits it probably by decreasing PKB level of phosphorylation.
  • 基因功能参考文献：
    1. we firstly revealed the association of CKIP-1 rs2306235 polymorphism with an increased risk of CHF in Chinese Han populations. PMID: 28402261
    2. we provided the first line of evidence that CKIP-1 regulated megakaryocytic differentiation and CKIP-1 knockout mice showed defective megakaryopoiesis and platelet production, suggesting a novel role for CKIP-1 in hematopoietic differentiation. PMID: 28404913
    3. these data integrated CKIP-1 expression and function as a novel transcriptional regulator of macrophage polarization. PMID: 28212865
    4. the increased PLEKHO1 could suppress Smad-dependent BMP signaling to inhibit bone formation during aging. PMID: 28083909
    5. High expression levels of CKIP1 were associated with the development of lung cancer, and that CKIP1 knockdown may block tumor cell growth mainly by promoting cell apoptosis. PMID: 25966172
    6. New light on the molecular mechanism of cell adhesion-mediated drug resistance in Non-Hodgkin's Lymphoma (NHL) and targeting CKIP-1 may be a novel therapeutic target for NHL. PMID: 27840970
    7. There is a major role of the CK2alpha-interacting protein CKIP-1 in activation of PAK1 for neoplastic prostate cells transformation. PMID: 26160174
    8. CKIP-1 interacts with CARMA1 and has an inhibitory effect on PKCtheta;-CBM-NF-kappaB signaling. PMID: 24465689
    9. CKIP-1 controlled Smurf1 expression in colon cancer PMID: 23995790
    10. overexpression triggers classical monocyte activation and transactivation of the TNF promoter PMID: 23583459
    11. CKIP-1, through its leucine zipper, interacts with the Rpt6 and mediates the Smurf1-Rpt6 interaction. PMID: 23032291
    12. CKIP-1 is involved in various important signaling pathways, controlling cell growth, apoptosis, differentiation, cytoskeleton and bone formation--REVIEW PMID: 22878216
    13. The N-terminal PH domain and C-terminal auto-inhibitory region of CKIP-1 coordinate to determine its subcellular localization and the nucleus-plasma membrane shuttling. PMID: 20171213
    14. examination of CK2alpha for a region that mediates interactions with CKIP-1 revealed a putative HIKE domain; this motif by itself may not be sufficient to mediate interactions. PMID: 15254037
    15. CKIP-1 plays a role in the regulation of the actin cytoskeleton through its interactions with actin capping protein. PMID: 15831458
    16. Data provide evidence that ATM, a predominantly nuclear kinase, could be relocalized to the plasma membrane by CKIP-1. PMID: 16325375
    17. CKIP-1 has a role in cell morphology that depends on its interaction with actin-capping protein PMID: 16987810
    18. The results provide a novel role of CKIP-1 in cytokine signaling response and the biochemical mechanism, by which two previously identified modulators IFP35 and Nmi are involved via interactions. PMID: 17197158
    19. Results indicate that CKIP-1, a novel Akt PH domain-interacting protein, would be a candidate of tumor suppressor with an Akt inhibitory function. PMID: 17942896
    20. CKIP-1 is cleaved by caspase-3 during apoptosis. C-terminal fragments translocate to the cytoplasm and nucleus and inhibit AP-1 activity via direct interaction with c-Jun and JunD. CKIP-1 also forms a feedback with caspase-3 and enhances apoptosis. PMID: 15706351
    21. CKIP-1 could interact with ATM and recruits nuclear ATM proteins partially to the plasma membrane. The results provide the first evidence that ATM, a predominantly nuclear kinase, could be re-localized to the plasma membrane. PMID: 16325375

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  • 亚细胞定位：
    Cell membrane; Peripheral membrane protein. Nucleus. Cytoplasm.
  • 组织特异性：
    Abundantly expressed in skeletal muscle and heart, moderately in kidney, liver, brain and placenta and sparingly in the pancreas and lung. Easily detectable in cell lines such as MOLT-4, HEK293 and Jurkat.
  • 数据库链接：

    HGNC: 24310

    OMIM: 608335

    KEGG: hsa:51177

    STRING: 9606.ENSP00000358120

    UniGene: Hs.438824