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  4. Recombinant Human Pre-mRNA-splicing regulator WTAP (WTAP)

Recombinant Human Pre-mRNA-splicing regulator WTAP (WTAP)

  • 货号：
    CSB-YP618970HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP618970HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP618970HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP618970HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP618970HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    WTAP
  • Uniprot No.：
  • 别名：
    DKFZp686F20131; Female-lethal(2)D homolog; FL2D_HUMAN; hFL(2)D; KIAA0105; MGC3925; Mum2; OTTHUMP00000017522; OTTHUMP00000017523; PNAS 132; PNAS-132; PNAS132; Pre mRNA splicing regulator WTAP; Pre-mRNA-splicing regulator WTAP; Putative pre mRNA splicing regulator female lethal 2D homolog; Putative pre mRNA splicing regulator female lethal(2D); Putative pre-mRNA splicing regulator female lethal(2D) homolog; putative pre-mRNA splicing regulator female-lethal(2D); Wilms tumor 1 associated protein; Wilms tumor 1 associating protein; Wilms tumor 1-associating protein; Wilms' tumor 1 associating protein; Wilms' tumour 1-associating protein; WT1 associated protein; WT1-associated protein; WT1-associating protein; wtap
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-396
  • 氨基酸序列
    MTNEEPLPKK VRLSETDFKV MARDELILRW KQYEAYVQAL EGKYTDLNSN DVTGLRESEE KLKQQQQESA RRENILVMRL ATKEQEMQEC TTQIQYLKQV QQPSVAQLRS TMVDPAINLF FLKMKGELEQ TKDKLEQAQN ELSAWKFTPD SQTGKKLMAK CRMLIQENQE LGRQLSQGRI AQLEAELALQ KKYSEELKSS QDELNDFIIQ LDEEVEGMQS TILVLQQQLK ETRQQLAQYQ QQQSQASAPS TSRTTASEPV EQSEATSKDC SRLTNGPSNG SSSRQRTSGS GFHREGNTTE DDFPSSPGNG NKSSNSSEER TGRGGSGYVN QLSAGYESVD SPTGSENSLT HQSNDTDSSH DPQEEKAVSG KGNRTVGSRH VQNGLDSSVN VQGSVL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Associated component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of RNAs, a modification that plays a role in the efficiency of mRNA splicing and RNA processing. Required for accumulation of METTL3 and METTL14 to nuclear speckle. Acts as a mRNA splicing regulator. Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability. Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes.
  • 基因功能参考文献：
    1. WTAP was over-expressed in glioma tissues, and the expression was closely correlated with glioma grade. PMID: 27370540
    2. WTAP as a regulatory subunit is required for formation of a functional m6A methyltransferase complex including METTL3 and METTL14, which play an important role in the regulation of gene expression and alternative splicing. PMID: 24407421
    3. WTAP regulates migration and invasion of cholangiocarcinoma cells. PMID: 23354623
    4. WTAP has an important role in abnormal proliferation and arrested differentiation of leukemia cells, and WTAP is a novel client protein of Hsp90 PMID: 24413322
    5. the WTAP complex is a novel component of the RNA processing machinery, implying an important role in both posttranscriptional control and cell cycle regulation. PMID: 24100041
    6. WTAP overexpression made cancer cells more tumorigenic PMID: 22957919
    7. WTAP level in poodocytes is a valuable alternative technique in the study of podocyte injury in diabetic nephropathy. PMID: 19969384
    8. WTAP protein involved in the morphogenesis of the genitourinaty tract and in the etiology of isolated hypospadias. PMID: 14675924
    9. WTAP and ATF3 Transcription Factor are activated in Wilms' tumor and accelerate tumorigenesis. PMID: 16912181
    10. WTAP as an essential factor for the stabilization of cyclin A2 mRNA, thereby regulating G2/M cell-cycle transition PMID: 17088532
    11. WTAP is a novel regulator of the cell cycle and cell survival and implicate a WTAP-WT1 axis as a novel pathway for controlling vascular smooth muscle cell phenotype. PMID: 17095724
    12. Nuclear degradation of Wilms tumor 1-associating protein and survivin splice variant switching underlie IGF-1-mediated survival PMID: 19605357

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  • 亚细胞定位：
    Nucleus speckle. Nucleus, nucleoplasm. Cytoplasm.
  • 蛋白家族：
    Fl(2)d family
  • 组织特异性：
    Ubiquitously expressed.
  • 数据库链接：

    HGNC: 16846

    OMIM: 605442

    KEGG: hsa:9589

    STRING: 9606.ENSP00000351141

    UniGene: Hs.446091