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  4. Recombinant Human Profilin-2 (PFN2)

Recombinant Human Profilin-2 (PFN2)

  • 货号：
    CSB-YP017825HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP017825HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP017825HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP017825HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP017825HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    D3S1319E; PFL; PFN 2; Pfn2; PROF2_HUMAN; Profilin II; Profilin-2; Profilin2; ProfilinII
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-140
  • 氨基酸序列
    MAGWQSYVDN LMCDGCCQEA AIVGYCDAKY VWAATAGGVF QSITPIEIDM IVGKDREGFF TNGLTLGAKK CSVIRDSLYV DGDCTMDIRT KSQGGEPTYN VAVGRAGRVL VFVMGKEGVH GGGLNKKAYS MAKYLRDSGF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG.
  • 基因功能参考文献：
    1. Study indicated that PFN2 may function as a negative regulator of colorectal cancer (CRC) metastasis and, thus, may represent a potential target for CRC therapy. PMID: 30015842
    2. High PFN2 expression might serve as a valuable predictor for poor overall survival of HNSC. DNA amplification and hypomethylation might be two mechanisms of PFN2 dysregulation. PMID: 29322815
    3. PFN2 gene, related to regulation of actin cytoskeleton in protein complex F, might play momentous roles in the initiation and development of consecutive Trauma-Induced Sepsis. PMID: 29642183
    4. PFN2 has a novel role in promoting Esophageal squamous cell carcinoma progression and metastasis. PMID: 27188458
    5. Study demonstrated that miR30a inhibits epithelial-mesenchymal transition (EMT) and invasion in highly invasive non-small cell lung cancer (NSCLC) cell lines via targeting PFN2 suggesting that miR30a with PFN2 may play an essential role in the development of NSCLC by modulating EMT and cell invasion. PMID: 28405690
    6. Disease causing mutations in inverted formin 2 regulate its binding to G-actin, F-actin capping protein (CapZ alpha-1) and profilin 2. PMID: 26764407
    7. Therefore, this study indicates that profilin 2 affects the metastatic potential and stemness of colorectal CSCs by regulating EMT- and stemness-related proteins. PMID: 25964982
    8. Pfn2 suppresses the recruitment of HDAC1 to Smad2 and Smad3 promoters, increasing their expression, and, in turn, enhancing TGFB1 induced epithelial-mesenchymal transformation and VEGF and CTGF production. PMID: 26354229
    9. Oral squamous cell carcinomas with weak PFN2 expression were associated with a significantly worse prognosis than strongly expressed tumours. PMID: 21725608
    10. These findings raise the possibility that Rgl3 mediates interaction between Ras/Rap-family proteins and profilin II, an important activator of actin polymerization. PMID: 17382517

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  • 亚细胞定位：
    Cytoplasm, cytoskeleton.
  • 蛋白家族：
    Profilin family
  • 组织特异性：
    Highly expressed in brain, skeletal muscle and kidney and less strongly in heart, placenta, lung and liver.
  • 数据库链接：

    HGNC: 8882

    OMIM: 176590

    KEGG: hsa:5217

    STRING: 9606.ENSP00000239940

    UniGene: Hs.91747