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  4. Recombinant Human Prostacyclin synthase (PTGIS), partial

Recombinant Human Prostacyclin synthase (PTGIS), partial

  • 货号：
    CSB-YP624112HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP624112HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP624112HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP624112HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP624112HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    PTGIS
  • Uniprot No.：
  • 别名：
    CYP8; CYP8A1; Cytochrome P450, family 8, subfamily A, polypeptide 1; EC 5.3.99.4; MGC126858; MGC126860; OTTHUMP00000031777; PGIS; Prostacyclin synthase; Prostaglandin I2 (prostacyclin) synthase; Prostaglandin I2 synthase; PTGI; PTGIS; PTGIS_HUMAN
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the biosynthesis and metabolism of eicosanoids. Catalyzes the isomerization of prostaglandin H2 to prostacyclin (= prostaglandin I2), a potent mediator of vasodilation and inhibitor of platelet aggregation. Additionally, displays dehydratase activity, toward hydroperoxyeicosatetraenoates (HPETEs), especially toward (15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoate (15(S)-HPETE).
  • 基因功能参考文献：
    1. In examining the tumor suppressor PTGIS as a potential biomarker for treatment with either 5-Aza-dC or TSA, 5-Aza-dC effectively stabilized cell cycling, restricted genetic instability and derepressed PTGIS expression PMID: 26130461
    2. Based on the predicted crystal structure of CYP8A1( *)5 using the Molecular Operating Environment platform, the distance from CYP8A1 Cys441 to the heme was altered with a significantly changed binding free energy for the mutant protein. PMID: 25623425
    3. results indicate that PGIS expression was associated with radiotherapy efficiency PMID: 25256272
    4. Type-2 diabetes is associated with higher COX-2 expression, but lower eNOS and PGIS expression in subcutaneous arteries. PMID: 24225501
    5. In a case-control study unaffected carriers of a BMPR2 mutation, linked to pulmonary artery hypertension (PAH), were most often found to have prostacyclin synthase promoter sequence variants, which were thought to protect against PAH. PMID: 24605778
    6. These findings suggest that PGIS is induced by hypoxia and regulates the expression of VEGF in fibroblasts. PMID: 23807031
    7. High PTGIS expression is associated with colorectal cancer hepatic metastasis. PMID: 22219064
    8. The CC genotype of C1117A polymorphism is associated with higher risk of LM-CAD PMID: 22072641
    9. Overexpression of PTGIS is associated with liver metastasis in colon cancer. PMID: 22109564
    10. diabetes preferentially increases PGIS nitration that is associated with excessive vascular inflammation in atherosclerotic carotid arteries from patients with type 2 diabetes PMID: 20348234
    11. Review: PGI2 function depends on the different alleles of the PTGIS gene and that they may influence the risk of cardiovascular disease. PMID: 20357747
    12. PGIS overexpression apparently protects insulin-producing cells against cytokine toxicity via suppression of endoplasmic reticulum and mitochondrial stress-mediated cell death pathways PMID: 20159982
    13. MI is associated with the CC genotype of rs5629 in the human CYP8A1 gene. The A-C-T haplotype appears to be a useful genetic marker and the C-T-T haplotype might be a protective factor of MI in Chinese people. PMID: 19147528
    14. Association study between a novel single nucleotide polymorphism of the promoter region of the prostacyclin synthase gene and essential hypertension. PMID: 11924728
    15. NMR and circular dichroism solution structure of microsomal PGIS PMID: 12193162
    16. the fallopian tube expresses both COX isoforms and PGIS PMID: 12213900
    17. the PGIS gene has a splicing mutation in familial hypertension PMID: 12372404
    18. Cyclic mechanical stretch augmented prostacyclin synthase promoter activity, via activation of activator protein-1 site, and prostacyclin synthase mRNA and protein expression in cultured human myometrial cells PMID: 12414894
    19. gestational age decrease in the inhibitory prostaglandin I2 synthase is consistent with lessening of its influence in myometrium at the time of labor PMID: 12414902
    20. effect of overexpression of this enzyme transfected into vascular smooth muscle cells in rats PMID: 12664597
    21. residue(s) within helix F/G loop of PGIS may be involved in forming the substrate access channel and located in a position that influences the membrane-bound PGIS catalytic function PMID: 12741817
    22. splicing mutation found in hypertension PMID: 12751759
    23. We successfully identified haplotypes of the PGIS gene, and these haplotypes were not associated with essential hypertension. PMID: 12924623
    24. results suggest specific VNTR polymorphism in the 5'-upstream promoter region of the PGIS gene regulated prostacyclin production, but did not seem to be associated with development of CTEPH (chronic thromboembolic pulmonary hypertension) PMID: 15182267
    25. Epigenetic inactivation of the PTGIS gene is a recurrent alteration in colorectal carcinogenesis PMID: 16007128
    26. The two major genetic loci Pgis1 and Pgis2 of murine spondylitis are homologous to chromosome regions in human genome, which control ankylosing spondylitis in human patients. PMID: 16081819
    27. PGIS has a typical low-spin heme with a hydrophobic active site. PMID: 16406803
    28. Dengue virus could promote the expression of PGIS mRNA in HUVEC and increase the level of PGI(2), which may increase the vascular permeability. PMID: 17210107
    29. In vitro shear stress can promote PGI(2 )secretion by human endothelial progenitor cells. PMID: 17221326
    30. Adenovirus PGIS gene transfer reduced PPAR delta expression and inhibited neointimal formation after balloon injury in accordance with the reduction in the phosphorylation of p38 MAPK PMID: 17303142
    31. PGIS promoter haplotype can play an important role in the predisposition for lung cancer and CpG methylation provides an epigenetic mechanism for the down-regulated PGIS expression. PMID: 17374734
    32. An intramuscular injection of AAV-PGIS prevents monocrotaline-pulmonary arterial hypertension in rats and provides a new therapeutic alternative for preventing pulmonary arterial hypertension in humans. PMID: 17635855
    33. These studies have provided the structural information for the interaction of the PGIS with its substrate mimic. PMID: 18081314
    34. Populations with both COX-2 -765GG and PGIS CC genotypes were more at risk for myocardial infarction. PMID: 19040046
    35. PTGIS genetic variation is associated with incident myocardial infarction. Carriers of rs20417C allele may derive greater benefits from aspirin use. PMID: 19046748
    36. The CC genotype and C allele of the prostacyclin synthase gene might be a risk factor of MI in Uigur population in Xinjiang. PMID: 19065539
    37. Characterization of the recombinant PGIS intermediates is reported in reactions with other peroxides, peracetic acid, and iodosylbenzene, providing a mechanistic model of a peroxidase reaction catalyzed by the class III cytochromes P450. PMID: 19187034
    38. rs237484 is in proximity to the potassium voltage gate channel gene (KCNB1) and close to the prostaglandin I2 (prostacyclin) synthase gene (PTGIS). PMID: 19265782
    39. The CC genotype of CYP8A1 is associated with myocardial infarction(MI), respectively. PMID: 19327107
    40. In the Uigur population CC genotype of rs5629 and the C-T-T haplotype of prostacyclin synthase gene are associated with myocardial infarction but the A-C-T haplotype of prostacyclin synthase gene might be a protective factor. PMID: 19719985

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  • 相关疾病：
    Essential hypertension (EHT)
  • 亚细胞定位：
    Endoplasmic reticulum membrane; Single-pass membrane protein.
  • 蛋白家族：
    Cytochrome P450 family
  • 组织特异性：
    Widely expressed; particularly abundant in ovary, heart, skeletal muscle, lung and prostate.
  • 数据库链接：

    HGNC: 9603

    OMIM: 145500

    KEGG: hsa:5740

    STRING: 9606.ENSP00000244043

    UniGene: Hs.302085