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  4. Recombinant Human Protein-L-isoaspartate (D-aspartate) O-methyltransferase (PCMT1)

Recombinant Human Protein-L-isoaspartate (D-aspartate) O-methyltransferase (PCMT1)

  • 货号：
    CSB-YP017618HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP017618HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP017618HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP017618HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP017618HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    PCMT1
  • Uniprot No.：
  • 别名：
    PCMT1; Protein-L-isoaspartate(D-aspartate) O-methyltransferase; PIMT; EC 2.1.1.77; L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl/D-aspartyl methyltransferase; Protein-beta-aspartate methyltransferase
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-227
  • 氨基酸序列
    AWKSGGASH SELIHNLRKN GIIKTDKVFE VMLATDRSHY AKCNPYMDSP QSIGFQATIS APHMHAYALE LLFDQLHEGA KALDVGSGSG ILTACFARMV GCTGKVIGID HIKELVDDSV NNVRKDDPTL LSSGRVQLVV GDGRMGYAEE APYDAIHVGA AAPVVPQALI DQLKPGGRLI LPVGPAGGNQ MLEQYDKLQD GSIKMKPLMG VIYVPLTDKE KQWSRWK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Initiates the repair of damaged proteins by catalyzing methyl esterification of L-isoaspartyl and D-aspartyl residues produced by spontaneous isomerization and racemization of L-aspartyl and L-asparaginyl residues in aging peptides and proteins. Acts on EIF4EBP2, microtubule-associated protein 2, calreticulin, clathrin light chains a and b, Ubiquitin C-terminal hydrolase isozyme L1, phosphatidylethanolamine-binding protein 1, stathmin, beta-synuclein and alpha-synuclein.
  • 基因功能参考文献：
    1. decrease of PCMT1 significantly increased the proportion of D-Asp residues in PHB1 and had significant and fatal impacts on morphology and functions of the mitochondria, such as ATP production and the mitochondrial fusion-fission system PMID: 27327778
    2. PIMT heterozygosity for R36C, G175R, R17H, or R17S would be detrimental to successful aging, whereas homozygosity (should it ever occur) would produce devastating neuropathology PMID: 28100787
    3. Strong PIMT expression was a predictive marker of poor prognosis for surgically resected lung adenocarcinoma. PMID: 26997432
    4. The data of this study indicated that DA-associated PIMT downregulation is an important event contributing to neuronal cell death PMID: 25800307
    5. ERK2-mediated phosphorylation of transcriptional coactivator binding protein PIMT/NCoA6IP at Ser298 augments hepatic gluconeogenesis. PMID: 24358311
    6. Overexpression of PCMT1 attentuates Mst1 kinase activation and its apoptotic effects in response to hypoxia-induced injury in cardiomyocytes. PMID: 23647599
    7. Data indicate that human PROTEIN ISOASPARTYL METHYLTRANSFERASE (PIMT) can initiate isoAsp conversion to Asp, and is able to restore Arabidopsis PRH75's complex biochemical activity provided isoAsp formation has not led to conformational alterations. PMID: 23903319
    8. Data show six differentially expressed proteins were identified as HSP70, PPIA and alpha-Enolase (up-regulated) S100-A9, PIMT and beta-5 tubulin (down-regulated), most of which had been shown to play a potential role in the pathogenesis of atherosclerosis. PMID: 21839816
    9. The results implied that maternal polymorphisms in PCMT1 might be a potential genetic risk factor for isolated anencephaly in the Chinese population of Lvliang. PMID: 22647835
    10. Study provides new insight into the molecular mechanisms by which PIMT suppresses the p53 activity through carboxyl methylation, and suggests a therapeutic target for cancers. PMID: 22735455
    11. PIMT may act as a co-activator in ERalpha-mediated transcription of TFF1 through its recruitment to the promoter via interacting with ERalpha. PMID: 22382029
    12. Control of PCMT1 expression by microRNA 15a/16-1 may thus represent a late checkpoint in apoptosis regulation PMID: 22033921
    13. A tight cross-regulation exists between ERK and PIMT in regards to their activation and expression during the epithelial mesenchymal transition. PMID: 21841813
    14. study demonstrates a novel role for PIMT as a negative regulator of Abeta peptide formation and a potential protective factor in the pathogenesis of Alzheimer disease PMID: 21372823
    15. Protein L-Isoaspartyl Methyltransferase PMID: 11792715
    16. crystal structure complexed with adenosyl homocysteine (AdoHcy) to 1.6-A resolution PMID: 11847284
    17. Our results showed that the Ile120Val polymorphism of PCMT1 gene is a genetic modifier for the risk of spina bifida. Val/Val genotype was associated with a reduction in risk for spina bifida. PMID: 16256389
    18. A potential role for PIMT in biological processes such as wound healing, cell migration, and tumor metastasis dissemination. PMID: 17167531
    19. These results suggest that PIMT repair of abnormal proteins is necessary to maintain normal MAPK signaling. PMID: 18381200
    20. Four polymorphisms in the protein L-isoaspartyl-O-methyltransferase (PCMT1) gene, encoding a protein repair enzyme, are associated with premature ovarian failure (POF). PMID: 18582870

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  • 亚细胞定位：
    Cytoplasm, cytosol.
  • 蛋白家族：
    Methyltransferase superfamily, L-isoaspartyl/D-aspartyl protein methyltransferase family
  • 数据库链接：

    HGNC: 8728

    OMIM: 176851

    KEGG: hsa:5110

    STRING: 9606.ENSP00000356348

    UniGene: Hs.279257