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  4. Recombinant Human Protein S100-A1 (S100A1), partial

Recombinant Human Protein S100-A1 (S100A1), partial

  • 货号：
    CSB-EP020622HU
  • 规格：
    ￥1344
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    Bpb; NEF; Protein S100-A1; S-100 protein alpha chain; S-100 protein subunit alpha; S100 alpha; S100 beta; S100 calcium binding protein A1; S100 calcium binding protein B; S100 calcium-binding protein A1; S100 protein alpha polypeptide; S100A; s100a1; S100B; S100beta; S10A1_HUMAN
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 来源：
    E.coli
  • 分子量：
    26.4kDa
  • 表达区域：
    2-94aa
  • 氨基酸序列
    GSELETAMETLINVFHAHSGKEGDKYKLSKKELKELLQTELSGFLDAQKDVDAVDKVMKELDENGDGEVDFQEYVVLVAALTVACNNFFWENS
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-SUMO-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Small calcium binding protein that plays important roles in several biological processes such as Ca(2+) homeostasis, chondrocyte biology and cardiomyocyte regulation. In response to an increase in intracellular Ca(2+) levels, binds calcium which triggers conformational changes. These changes allow interactions with specific target proteins and modulate their activity. Regulates a network in cardiomyocytes controlling sarcoplasmic reticulum Ca(2+) cycling and mitochondrial function through interaction with the ryanodine receptors RYR1 and RYR2, sarcoplasmic reticulum Ca(2+)-ATPase/ATP2A2 and mitochondrial F1-ATPase. Facilitates diastolic Ca(2+) dissociation and myofilament mechanics in order to improve relaxation during diastole.
  • 基因功能参考文献：
    1. Data suggest that TRPM4 exhibits binding sites for calmodulin (CaM) and S100 calcium-binding protein A1 (S100A1) located in very distal part of TRPM4 N-terminus. (TRPM4 = transient receptor potential cation channel subfamily M member 4) PMID: 29240297
    2. Data found that S100A1 was upregulated in hepatocellular carcinoma (HCC) tissues, and its upregulation was associated with large tumor size, low differentiation, and shorter survival rates. Further results supports the hypothesis that S100A1 functions as an oncogene and may be a biomarker for the prognosis of patients with HCC. S100A1 exerted its oncogenic function by interacting with LATS1 and activating Hippo pathway. PMID: 29901195
    3. The results indicate that changes in the circulating level of S100A1 protein occur in metabolic syndrome patients. The strong correlation between serum zinc-alpha2-glycoprotein and S100A1 might suggest that production or release of these two proteins could be related mechanistically. PMID: 28825380
    4. The results indicated that S100A1 enhanced the ovarian cancer cell proliferation and migration. PMID: 28595036
    5. found that S100B plays a crucial role in blocking the interaction site between RAGE V domain and S100A1. A cell proliferation assay WST-1 also supported our results. This report could potentially be useful for new protein development for cancer treatment PMID: 29444082
    6. Study provides evidence that mir-363 and its target S100A1 are under the regulatory function of FOXD2-AS1 aggravating nasopharyngeal carcinoma carcinogenesis. PMID: 29248577
    7. X-ray crystal structure of human calcium-bound S100A1 has been reported. PMID: 28368280
    8. a molecular mechanism for the potential regulation of TRPM1 by S100A1 PMID: 27435061
    9. Data suggest that calcium signaling plays important role in prevention of protein misfolding; complexes of S100A1 and STIP1 are key players in this pathway; the stoichiometry of S100A1/STIP1 interaction appears to be three S100A1 dimers plus one STIP1 monomer; each S100A1-STIP1-binding interaction is entropically driven. (S100A1 = S100 calcium binding protein A1; STIP1 = stress-induced-phosphoprotein 1) [REVIEW] PMID: 28819010
    10. Data suggest that three dimers of S100A1 (S100 calcium binding protein A1) associate with one molecule of STIP1 (stress-inducible phosphoprotein 1) in a calcium-dependent manner; individual STIP1 TPR (tetratricopeptide repeat) domains, TPR1, TPR2A and TPR2B, bind a single S100A1 dimer with significantly different affinities; TPR2B domain possesses highest affinity for S100A1. PMID: 28408431
    11. Results identified amino acids motif in S100A1 for protein binding to 2-oxohistidine which appears to be an evolutionarily conserved capacity from bacteria to human. PMID: 27644758
    12. a correlation between S100B + A1-positive Human Articular Chondrocytes in monolayer culture and their neochondrogenesis capacity in pellet culture, is reported. PMID: 27861869
    13. In line with these observations, rhesus monkey rhadinovirus infection resulted in rapid degradation of SP100, followed by degradation of PML and the loss of ND10 structures, whereas the protein levels of ATRX and DAXX remained constant. PMID: 27356898
    14. High Serum protein S100 levels are associated with postoperative delirium after off-pump coronary artery bypass surgery. PMID: 26943607
    15. molecular dynamics simulations of S100A1 in the apo/holo (Ca(2+)-free/bound) states, is reported. PMID: 26958883
    16. The relationship between the degree of infiltration by S100-positive (S100+) dendritic cells and prognostic factors, including histological subtype, histological grade, peritumor inflammatory infiltration, and stromal desmoplasia, were examined. PMID: 25611268
    17. In this review, we aim to describe the molecular basis and regulatory function of S100A1--{REVIEW} PMID: 25157660
    18. Patients with acute myocardial infarction (MI) showed significantly increased S100A1 serum levels. S100A1 signaling in cardiac fibroblasts occurs through endosomal TLR4/MyD88. PMID: 24833748
    19. hypoxia-induced MiR-138 is an essential mediator of EC dysfunction via its ability to target the 3'UTR of S100A1. PMID: 24244340
    20. It was suggested that S100A1 and S100B be used as markers to develop potency assays for cartilage regeneration cell therapies, and as a redifferentiation readout in monolayer cultures aiming to investigate stimuli for chondrogenic induction. PMID: 24402969
    21. Twenty-seven out of thirty-two (84.38%) cases of serous ovarian carcinoma were found to express S100A1. S100A1 expression was observed in one out of the two mucinous and three out of the six endometroid ovarian carcinomas. PMID: 24501865
    22. The triple mutation Arg852/Lys859/Arg860 exhibited significant disruption of the binding of S100A1 to TRPC6 implicating their involvement in the binding site. PMID: 23671622
    23. For autopsy material, all human cases of definite myocardial infarction and suspected early infarction showed well-defined areas without S100A1 staining. PMID: 23683996
    24. This study showed that overexpression of S100A10, may play a role in irritable bowel syndrome, and that the IL10-819 CC is a candidate genotype for irritable bowel syndrome and ulcerative colitis. PMID: 23595519
    25. In this review we will focus on the roles of S100A proteins in intracellular and extracellular calcium signalling and homeostasis. PMID: 23662436
    26. the increased calcium binding affinity of S100A1 upon thionylation arises, most probably, from rearrangement of the hydrophobic core in its apo form PMID: 23351007
    27. Report downregulation of S100A1 expression in critical limb ischemia impairs postischemic angiogenesis via compromised proangiogenic endothelial cell function and nitric oxide synthase regulation. PMID: 23048072
    28. S-Nitrosylation of S100A1 increases Ca(2+) binding and tunes the overall protein conformation. PMID: 22989881
    29. Uremia clearance using NHD is accompanied by improvement in LV strain, rotation, reduction in mass and volume index. Dialysis downregulates genes for cardiomyocyte apoptosis and fibrosis and upregulates S100A1, which may improve LV contractility. PMID: 22647434
    30. Calmodulin and S100A1 protein interact with N terminus of TRPM3 channel. PMID: 22451665
    31. The three-dimensional structure of human apo(calcium free)S100A1 protein was determined by NMR spectroscopy PMID: 21296671
    32. Thermodynamics of zinc binding to human S100A2 PMID: 21090249
    33. S100A1, S100A2, S100A4, S100A6 and S100B interacted with MDM2 (2-125). PMID: 20591429
    34. A single phenyl-Sepharose column was sufficient for the purification of human S100A11 whereas HiTrap Q anion exchange followed by phenyl-Sepharose columns were required for the purification of S100A1. PMID: 20347987
    35. The expression of S100A1 was low in benign melanocytic tumours and increased in malignant melanomas PMID: 20042890
    36. S100A1B and S100BB are increased in urine collected from asphyxiated newborns who will develop hypoxic-ischemic encephalopathy (HIE) since first urination, and their measurement may be useful to early predict HIE. PMID: 19482672
    37. S100A1 is a specific and sensitive immunohistochemical marker to differentiate nephrogenic adenoma from prostatic adenocarcinoma PMID: 19384190
    38. The presence of the S100A1 in myocardial sarcoplasmic reticulum and myofibrils may be related to the known target proteins for S100A1 at these sites. PMID: 11829317
    39. Impaired cardiac contractility response to hemodynamic stress in S100A1-deficient mice. PMID: 11909974
    40. identification of the S100A1 C terminus (amino acids 75-94) and hinge region (amino acids 42-54) to differentially enhance sarcoplasmic reticulum Ca2+ release with a nearly 3-fold higher activity of the C terminus PMID: 12721284
    41. S100A1 has a reglatory role in the contraction-relaxation cycle in the human heart PMID: 12804600
    42. S100A1 protein serves as a cardioprotective factor in vitro PMID: 12960148
    43. identified S100A1, but not calmodulin or other S100 proteins, as a potent molecular chaperone and a new member of the Hsp70/Hsp90 multichaperone complex PMID: 14638689
    44. Synapsins and S100A1 interact in nerve terminals where coexpresssed; S100A1 cannot bind SV-associated synapsin I and may function as a cytoplasmic store of monomeric synapsin I; synapsin dimerization and interaction with S100A1 are mutually exclusive PMID: 15147519
    45. Results demonstrate that restoration of S100A1 protein levels in failing myocardium by gene transfer may be a novel therapeutic strategy for the treatment of heart failure. PMID: 15578088
    46. This study provides the first evidence that intracellular S100A1, depending on its subcellular location, modulates cardiac Ca2+-turnover via different Ca2+-regulatory proteins. PMID: 15654019
    47. S100A1 was expressed in 2/15 clear cell RCCs, 11/15 papillary RCCs, 7/8 oncocytomas and in 0/7 chromophobe RCCs. PMID: 15780567
    48. the three-dimensional structure of calcium-bound S100A1 was determined by multidimensional NMR spectroscopy and compared to the previously determined structure of apo-S100A1 PMID: 16169012
    49. S-100 protein expression in tumour cells was associated with significantly decreased survival PMID: 16760135
    50. analysis of the different reactivity pattern of S100A1 in the external auditory canal cholesteatoma PMID: 16969478

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  • 亚细胞定位：
    Cytoplasm. Sarcoplasmic reticulum. Mitochondrion.
  • 蛋白家族：
    S-100 family
  • 组织特异性：
    Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.
  • 数据库链接：

    HGNC: 10486

    OMIM: 176940

    KEGG: hsa:6271

    STRING: 9606.ENSP00000292169

    UniGene: Hs.515715