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  4. Recombinant Human Protein Tob1 (TOB1)

Recombinant Human Protein Tob1 (TOB1)

  • 中文名称：
    人TOB1重组蛋白
  • 货号：
    CSB-YP024037HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人TOB1重组蛋白
  • 货号：
    CSB-EP024037HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人TOB1重组蛋白
  • 货号：
    CSB-EP024037HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人TOB1重组蛋白
  • 货号：
    CSB-BP024037HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人TOB1重组蛋白
  • 货号：
    CSB-MP024037HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    TOB1
  • Uniprot No.：
  • 别名：
    TOB1; TOB; TROB1; Protein Tob1; Transducer of erbB-2 1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-345
  • 氨基酸序列
    MQLEIQVALN FIISYLYNKL PRRRVNIFGE ELERLLKKKY EGHWYPEKPY KGSGFRCIHI GEKVDPVIEQ ASKESGLDID DVRGNLPQDL SVWIDPFEVS YQIGEKGPVK VLYVDDNNEN GCELDKEIKN SFNPEAQVFM PISDPASSVS SSPSPPFGHS AAVSPTFMPR STQPLTFTTA TFAATKFGST KMKNSGRSNK VARTSPINLG LNVNDLLKQK AISSSMHSLY GLGLGSQQQP QQQQQPAQPP PPPPPPQQQQ QQKTSALSPN AKEFIFPNMQ GQGSSTNGMF PGDSPLNLSP LQYSNAFDVF AAYGGLNEKS FVDGLNFSLN NMQYSNQQFQ PVMAN
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Anti-proliferative protein; the function is mediated by association with deadenylase subunits of the CCR4-NOT complex. Mediates CPEB3-accelerated mRNA deadenylation by binding to CPEB3 and recruiting CNOT7 which leads to target mRNA deadenylation and decay.
  • 基因功能参考文献：
    1. MiR-590 targets Tob1 that is significantly decreased in patients with multiple sclerosis. PMID: 28947212
    2. The dual-luciferase reporter assay also confirmed that miR-25 harbored the A allele which caused an incapacitation of binding at the TOB1. In conclusion, rs41274221 in miR-25 was a subgroup which may protect the patients from further growth and metastasis of gastric cancer and might serve as a novel biomarker for the disease PMID: 26572149
    3. A novel role for TOB1 in mediating survival of estrogen-independent breast cancers. PMID: 26165839
    4. Reduced TOB1 expression in gastric adenocarcinoma is associated with the extent of differentiation and the TNM stage of gastric cancer. PMID: 25760308
    5. miR-25 promotes GC progression by directly downregulating TOB1 expression, and may be a noninvasive biomarker for the prognosis of GC patients. PMID: 25043310
    6. arsenite-induced oxidative stress inhibits deadenylation of mRNA primarily through downregulation of Tob and Pan3, both of which mediate the recruitment of deadenylases to mRNA PMID: 25446091
    7. Tob is a key factor in the regulation of c-myc gene expression, which is essential for cell growth. PMID: 23178487
    8. TOB1 demonstrated a radioprotective function in the immortalized normal human bronchial epithelial cell line. PMID: 23756562
    9. TOB1 modulates the radiosensitivity of lung cancer cells via the MAPK/ERK signaling pathway. PMID: 23589165
    10. Cdc7 phosphorylates and interacts with Tob to inhibit the Cul4-DDB1(Cdt2)-dependent Tob degradation. PMID: 23066029
    11. study demonstrates that the overexpression of Tob1 inhibits gastric cancer progression by activating Smad4- and inhibiting betacatenin-mediated signaling pathways PMID: 22710759
    12. TOB1 overexpression not only increased the expression of the phosphatase and tensin homolog (PTEN), an important tumor suppressor, but also regulated the downstream effectors in the PI3K/PTEN signaling pathway, including Akt, ERK1/2, etc. PMID: 22158108
    13. The antiproliferative and the mRNA deadenylation/decay-promoting effects of TOB1 and TOB2 proteins are linked. PMID: 22252318
    14. Silencing of HIC1 and TOB1 expression is a common occurrence in gastric cancer and may contribute to the development and progression of the disease. PMID: 21533545
    15. Longer survival was associated with hypomethylation at specific CpG sites (e.g. GREB1, TGIF and TOB1) and hypermethylation in other genes (e.g. TMCO5, PTPRN and GUCY2C). PMID: 21577013
    16. Together, these results indicate that Tob mediates the recruitment of Caf1 to the target of CPEB3 and elicits deadenylation and decay of the mRNA. PMID: 21336257
    17. Tob interactions with Caf1 and a C-terminal domain of PABPC1 PMID: 20595394
    18. functions as lateral signal transmitter PMID: 11904957
    19. Antiproliferative proteins of the BTG/Tob family are degraded by the ubiquitin-proteasome system. the C-terminal regions are necessary and sufficient to control the stabilities of BTG1, BTG2, Tob, and Tob2 proteins. PMID: 12135500
    20. identified as a MAPK substrate PMID: 12151396
    21. Decreased expression or phosphorylation status of tob protein is associated with lung cancer PMID: 14643028
    22. N-terminal region of TOB is a functional nuclear export signal. PMID: 15051490
    23. Tob is involved in the translational suppression of IL-2 mRNA in anergic T cells through its interaction with iPABP. PMID: 15676026
    24. Tob1 is a novel target for degradation by the SCF-Skp2 ubiquitin ligase in a cell line. PMID: 16951159
    25. Reverse-transcription polymerase chain reaction confirmed increased expression of GADD45A, BTG2, PDE4B, and CEBPD and downregulation of TOB1 in skeletal muscle intradialysis. PMID: 16997058
    26. Subtractive hybridization identified Twisted gastrulation (Tsg) as one of the genes suppressed by Tob. PMID: 17164348
    27. Data show that TOB enhances mRNA deadenylation in vivo, and that interaction with PABPC1 is necessary for TOB's deadenylation-enhancing effect. PMID: 17785442
    28. antiproliferative region of human Tob (residues 1-138) and intact hCaf1 were co-expressed in Escherichia coli, purified and successfully cocrystallized PMID: 18084094
    29. Tob associates with the CCR4-NOT complex. PMID: 18377426
    30. positive correlation of TOB1 phosphorylation status with proliferation marker Ki67 suggests that elevated TOB1 phosphorylation might abrogate the antiproliferative effect of TOB1 in breast cancer PMID: 19491269
    31. Down-regulation of TOB is associated with breast cancer tumorigenesis. PMID: 19569230

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    BTG family
  • 组织特异性：
    Ubiquitous.
  • 数据库链接：

    HGNC: 11979

    OMIM: 605523

    KEGG: hsa:10140

    STRING: 9606.ENSP00000268957

    UniGene: Hs.703321