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  4. Recombinant Human Protein Wnt-5a (WNT5A)

Recombinant Human Protein Wnt-5a (WNT5A)

In Stock
  • 货号：
    CSB-EP026138HU
  • 规格：
    ￥1344
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    hWNT 5A; hWNT5A; Protein Wnt 5a; Protein Wnt-5a; Protein Wnt5a; Wingless type MMTV integration site family member 5A; Wnt 5a; WNT 5A protein; WNT 5A protein precursor; WNT5A; WNT5A protein precursor; WNT5A_HUMAN
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    E.coli
  • 分子量：
    39.8 kDa
  • 表达区域：
    62-380aa
  • 氨基酸序列
    IIGAQPLCSQLAGLSQGQKKLCHLYQDHMQYIGEGAKTGIKECQYQFRHRRWNCSTVDNTSVFGRVMQIGSRETAFTYAVSAAGVVNAMSRACREGELSTCGCSRAARPKDLPRDWLWGGCGDNIDYGYRFAKEFVDARERERIHAKGSYESARILMNLHNNEAGRRTVYNLADVACKCHGVSGSCSLKTCWLQLADFRKVGDALKEKYDSAAAMRLNSRGKLVQVNSRFNSPTTQDLVYIDPSPDYCVRNESTGSLGTQGRLCNKTSEGMDGCELMCCGRGYDQFKTVQTERCHCKFHWCCYVKCKKCTEIVDQFVCK
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    The expression of recombinant human WNT5A protein includes the construction of the expression vector containing the recombinant DNA and the transformation of the expression vector into the E.coli, which provides a variety of macromolecules and components required for transcription and translation. The recombinant DNA was formed by fusing the N-terminal 6xHis tag sequence to the designated sequence encoding the 62-380aa of the human WNT5A protein. This N-terminal GST-tagged recombinant human WNT5A protein is also characterized by high purity, >85%. Under SDS-PAGE condition, this recombinant WNT5A protein migrated to the band of about 40 kDa molecular weight. It may have applications in WNT5A-mediated cancer research.

    WNT5B is considered to be a non-classical Wnt protein in the process of fat differentiation. Wnt protein family includes at least 19 members, which is a group of secretory glycoproteins rich in cysteine and participates in many biological processes such as embryonic development, cell proliferation and differentiation, and even tumor occurrence. Wnt5b is a member of the same family of proteins as Wnt5a. Evidence has accumulated that Wnt5b expression is involved in mouse chondrocyte maturation. Wnt5b were reported to be highly expressed in head and neck squamous cell carcinomas. Other studies also found highly expressed in breast cancer with high invasive activity. Of late, the role of Wnt5b overexpression in cancer aggressiveness has been identified. For example, in lung cancer and pancreatic cancer cells, upregulated Wnt5b has been observed, but the biochemical properties and cancer-associated activities of Wnt5b have not been well characterized.

  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression. Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor. Mediates motility of melanoma cells. Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle. Inhibits type II collagen expression in chondrocytes.
  • 基因功能参考文献：
    1. Impaired Wnt5a signaling is associated with poor placentation and subsequent development of preeclampsia. PMID: 30177057
    2. Decreased hepatic Wnt5a signaling is associated with hepatocellular carcinoma progression and poor prognosis. PMID: 29709351
    3. Annotation of rs11918967 in WNT5A in tissues might be related to obesity. PMID: 28272483
    4. Wnt5a expression is critical for proliferation of RL and VZ progenitors and Purkinje cell dendritogenesis during early embryonic development resulting in retarded development of cerebellum during postnatal stages. PMID: 28205531
    5. Study shows that Wnt5a is upregulated in invasive glioblastoma tissues, and demonstrates that it may regulate the invasion of glioblastoma cells, at least in part via the Daam1/RhoA signaling pathway. PMID: 29207169
    6. The findings suggest that Wnt5a expression may be involved in the inhibition of cell differentiation and the induction of an inflammatory response. PMID: 29286164
    7. High WNT5A expression is associated with gliomas PMID: 28627699
    8. Wnt5a promotes epithelial-to-mesenchymal transition and metastasis in non-small-cell lung cancer, which is involved in the activation of beta-catenin-dependent canonical Wnt signaling. PMID: 29054966
    9. This study suggests that methylation of Wnt pathway genes, in addition to known CpG island methylator phenotype markers, may help predict treatment outcome and survival in patients with CRC[colorectal cancer. PMID: 29869456
    10. non-canonical Wnt5a signalling could play a role in early human trophoblast development by promoting cell proliferation and survival. PMID: 27311852
    11. These findings suggest that WNT-5A modulates fundamental mechanisms that affect airway smooth muscle contraction and thus may be of relevance for airway hyperresponsiveness in asthma. PMID: 27468699
    12. We performed immunohistochemistry for Ki67, p16INK4a, and WNT5A in human HPs ( hyperplastic polyps), sessile serrated adenomas/polyps (SSA/Ps), and traditional serrated adenomas (TSAs) .The distribution of Ki67 and p16INK4a positive cells in TSAs was different from that in HPs and SSA/Ps. PMID: 28627675
    13. Wnt5a-Ror2 signaling enhanced tongue SCC cell aggressiveness and promoted production of MMP-2 following DeltaNp63beta-mediated EMT PMID: 28559016
    14. Here, the s define WNT5A, a non-canonical Wnt ligand implicated in epithelial differentiation, repair, and cancer, as a direct transcriptional target that is activated by KLF4 in squamous epithelial cells. PMID: 27184424
    15. These findings support that Wnt5a-Ror2 signaling plays a role in UC, support the potential use of Wnt5a as a prognostic marker and provide evidence that Wnt5a signaling may be used as an effective molecular target for novel therapeutic tools. PMID: 28427201
    16. We show that rosiglitazone increases klotho and decreases Wnt5A in tumor cells, reducing the burden of both BRAF inhibitor-sensitive and BRAF inhibitor-resistant tumors in aged, but not young mice. However, when used in combination with PLX4720, tumor burden was reduced in both young and aged mice, even in resistant tumors PMID: 28232477
    17. Serum Wnt5a is elevated and associated with disease severity in heart failure patients. PMID: 28357477
    18. In squamous/adenosquamous carcinoma and adenocarcinoma of the gallbladder positive ROR2 or WNT5a expression is generally associated with a poor prognosis. PMID: 28465645
    19. The Wnt5a and its signaling pathway can regulate fundamental cellular processes, including specification of cell fate, proliferation, and survival. PMID: 28641961
    20. Results show that PTEN and WNT5A expression are directly repressed by miR-26b which promotes colorectal cancer metastasis. PMID: 29160937
    21. The relationship between Wnt5a protein expression and histone H4K20me1 was analyzed. Recruitment of H4K20me1 and SET8 to the Wnt5a promoter and coding regions wa investigated. Results demonstrated that the expression levels of Wnt antagonists were generally low in acute myeloid leukemia (AML), but showed differential expression in acute lymphocytic leukemia (ALL). PMID: 28440495
    22. WNT5A and IL-6 are connected through a positive feedback loop in melanoma cells PMID: 27191257
    23. this study reveals that 14-3-3zeta plays a critical role in Wnt5a/ROR1 signaling, leading to enhanced CLL migration and proliferation. PMID: 28465528
    24. these studies indicate HS1 plays an important role in ROR1-dependent Wnt5a-enhanced chemokine-directed leukemia-cell migration. PMID: 28465529
    25. Study reports that WNT5A bi-directionally regulates epithelial-mesenchymal transition (EMT) in mammary epithelial cells, thereby affecting their migration and invasion. However, the ability of WNT5A to inhibit breast cancer cell migration and invasion is an EMT-independent mechanism that, at least in part, can be explained by decreased CD44 expression. PMID: 27623766
    26. disruption of trans-spliced noncoding RNA RMST expression in human embryonic stem cells results in the upregulation of WNT5A, epithelial-to-mesenchymal transition, and lineage-specific genes/markers. PMID: 27090862
    27. Wnt5a suppressed osteoblastic differentiation through Ror2/JNK signaling in periodontal ligament stem cell-like cells. PMID: 28681925
    28. Genetic blockade of autophagy indicated an unexpected feedback loop whereby knocking down the autophagy factor ATG5 in Wnt5A(high) cells decreased Wnt5A and increased beta-catenin. PMID: 28887323
    29. Low WNT5A expression is associated with prostate cancer. PMID: 28748258
    30. WNT5A signaling regulates 15-PGDH expression. PMID: 27522468
    31. our study showed that, for the first time, different Wnt5a mRNA isoforms play distinct roles in colorectal cancer (CRC) and can be used as novel prognostic markers for CRC in the future. PMID: 28859077
    32. High WNT5a expression is associated with osteoarthritis. PMID: 28777797
    33. We conclude that in RS, WNT5A missense mutations have dominant neomorphic effects that interfere with the function of the wild-type protein. PMID: 28662348
    34. RSPO2 suppresses colorectal cancer metastasis by counteracting the Wnt5a/Fzd7-driven noncanonical Wnt pathway. PMID: 28600110
    35. High expression of WNT5A is associated with squamous cell lung carcinoma. PMID: 27876017
    36. Study shows that WNT5A stimulates dimerization of membrane-anchored FZD4 CRDs and oligomerization of full-length FZD4, which requires the integrity of CRD palmitoleoyl-binding residues. These results suggest that FZD receptors may form signalosomes in response to Wnt binding through the CRDs and that the Wnt palmitoleoyl group is important in promoting these interactions. PMID: 28546512
    37. Taken together with our previous findings, we have replicated our results from the rodent system in a novel human system. We have revealed a unique sequential cascade involving Atg10, Wnt5a, alpha1 integrin, and matrix metalloproteinase-3 in GS/BMP-4-induced differentiation of hiPS cells into odontoblast-like cells at a relatively early stage. PMID: 27639333
    38. Elevated WNT5A expression in obesity may function as a negative regulator of angiogenesis.NEW & NOTEWORTHY Wingless-related integration site 5a (WNT5A) negatively regulates adipose tissue angiogenesis via VEGF-A165b in human obesity. PMID: 28411232
    39. Wnt5a as a master regulator of brain invasion, specifically tumor-promoting stem-like characteristics (TPC), and they provide a therapeutic rationale to target it in patients with glioblastoma. PMID: 28011620
    40. inhibition of WNT-5A in vivo attenuated lung tissue destruction, improved lung function, and restored expression of beta-catenin-driven target genes and alveolar epithelial cell markers in the elastase, as well as in cigarette smoke induced models of COPD. PMID: 27979969
    41. Wnt5A/Ryk signaling might provide novel therapeutic strategies to prevent capillary leakage in systemic inflammation and septic shock. PMID: 27159116
    42. Profound vascular insulin resistance in the visceral adipose tissue arterioles of obese subjects was associated with up-regulated WNT5A-JNK signaling and impaired endothelial eNOS activation. PMID: 27688298
    43. Aberrant activation of WNT pathways in patients with ESRD significantly correlated with vascular calcification. PMID: 27156072
    44. Wnt5A could be a potential therapeutic target for reducing microvascular leakage and edema formation in Th2 driven inflammatory diseases. PMID: 27214384
    45. WNT5A and ROR2 are induced by inflammatory mediators through NF-kB and STAT3 transcription factors, and are involved in the migration of human ovarian cancer cell line SKOV-3. PMID: 28536612
    46. decreased UHRF1 expression is a key initial event in the suppression of DNMT1-mediated DNA methylation and in the consequent induction of senescence via increasing WNT5A expression PMID: 28100769
    47. TrpC5 causes a robust rise in [Ca2+]i, enhanced Wnt5a expression and nuclear translocation of beta-catenin, leading to reduced differentiation and enhanced cancer cell stemness. PMID: 27895148
    48. This study describes the localization and functional role of WNT-5A in human and mouse fibrotic livers. Hepatic WNT-5A expression parallels collagen type I expression. In vivo and in vitro, the myofibroblasts were identified as the key hepatic cells producing WNT-5A. WNT-5A is under control of TGF-beta and its activities are primarily profibrotic. PMID: 28057611
    49. this study shows that Wnt5a promotes differentiation of dendritic cells, but inhibits their maturation PMID: 27641635
    50. we confirmed the requirement of Wnt5a in the deferoxamine-mediated osteoblast-promoting effects by analyzing the matrix mineralization of Wnt5a-deficient cells. The promoting effect of deferoxamine on matrix mineralization in wild-type cells was completely abolished in Wnt5a(-/-) cells. PMID: 27540134

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  • 相关疾病：
    Robinow syndrome, autosomal dominant 1 (DRS1)
  • 亚细胞定位：
    Secreted, extracellular space, extracellular matrix. Secreted.
  • 蛋白家族：
    Wnt family
  • 组织特异性：
    Expression is increased in differentiated thyroid carcinomas compared to normal thyroid tissue and anaplastic thyroid tumors where expression is low or undetectable. Expression is found in thyrocytes but not in stromal cells (at protein level). Detected i
  • 数据库链接：

    HGNC: 12784

    OMIM: 164975

    KEGG: hsa:7474

    STRING: 9606.ENSP00000264634

    UniGene: Hs.643085