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  4. Human Protein Wnt-5a(WNT5A) ELISA kit

Human Protein Wnt-5a(WNT5A) ELISA kit

  • 中文名称：
    人Wnt-5a蛋白(WNT5A)酶联免疫试剂盒
  • 货号：
    CSB-EL026138HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human WNT5A ELISA Kit was designed for the quantitative measurement of Human WNT5A protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL .

  • 别名：
    hWNT 5A ELISA Kit; hWNT5A ELISA Kit; Protein Wnt 5a ELISA Kit; Protein Wnt-5a ELISA Kit; Protein Wnt5a ELISA Kit; Wingless type MMTV integration site family member 5A ELISA Kit; Wnt 5a ELISA Kit; WNT 5A protein ELISA Kit; WNT 5A protein precursor ELISA Kit; WNT5A ELISA Kit; WNT5A protein precursor ELISA Kit; WNT5A_HUMAN ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.156 ng/mL-10 ng/mL
  • 灵敏度：
    0.039 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Stem Cells
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human WNT5A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %92
    Range %90-95
    1:2Average %95
    Range %90-103
    1:4Average %84
    Range %80-92
    1:8Average %100
    Range %98-104
  • 回收率：
    The recovery of human WNT5A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9383-99
    EDTA plasma (n=4)9791-104
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    101.839 1.899 1.869 1.685
    51.571 1.583 1.577 1.393
    2.51.166 1.205 1.186 1.002
    1.250.732 0.781 0.757 0.573
    0.6250.448 0.462 0.455 0.271
    0.3120.325 0.341 0.333 0.149
    0.1560.236 0.247 0.242 0.058
    00.183 0.185 0.184 　
  • 本试剂盒所含材料：
    • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human WNT5A antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
    • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
    • One vial Biotin-labeled WNT5A antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
    • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
    • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
    • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
    • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
    • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
    • An instruction manual

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  • 本试剂盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

Does this Elisa kit, CSB-EL026138HU only react with Human cells and not mouse cells?

A:
Thanks for your inquiry! Protein Wnt-5a is protein macromolecule. The protein level AA sequence of different species (human and house) is different, and the structure is also various. Generally it does not make the protein cross-analysis with inter-species. Besides, based on the impact of kit applicability and sample matrix differences and antibody characteristics, we provide this target for different species. Here recommend you to use the corresponding kits for the required species.

靶点详情

  • 功能：
    Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression. Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor. Mediates motility of melanoma cells. Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle. Inhibits type II collagen expression in chondrocytes.
  • 基因功能参考文献：
    1. Impaired Wnt5a signaling is associated with poor placentation and subsequent development of preeclampsia. PMID: 30177057
    2. Decreased hepatic Wnt5a signaling is associated with hepatocellular carcinoma progression and poor prognosis. PMID: 29709351
    3. Annotation of rs11918967 in WNT5A in tissues might be related to obesity. PMID: 28272483
    4. Wnt5a expression is critical for proliferation of RL and VZ progenitors and Purkinje cell dendritogenesis during early embryonic development resulting in retarded development of cerebellum during postnatal stages. PMID: 28205531
    5. Study shows that Wnt5a is upregulated in invasive glioblastoma tissues, and demonstrates that it may regulate the invasion of glioblastoma cells, at least in part via the Daam1/RhoA signaling pathway. PMID: 29207169
    6. The findings suggest that Wnt5a expression may be involved in the inhibition of cell differentiation and the induction of an inflammatory response. PMID: 29286164
    7. High WNT5A expression is associated with gliomas PMID: 28627699
    8. Wnt5a promotes epithelial-to-mesenchymal transition and metastasis in non-small-cell lung cancer, which is involved in the activation of beta-catenin-dependent canonical Wnt signaling. PMID: 29054966
    9. This study suggests that methylation of Wnt pathway genes, in addition to known CpG island methylator phenotype markers, may help predict treatment outcome and survival in patients with CRC[colorectal cancer. PMID: 29869456
    10. non-canonical Wnt5a signalling could play a role in early human trophoblast development by promoting cell proliferation and survival. PMID: 27311852
    11. These findings suggest that WNT-5A modulates fundamental mechanisms that affect airway smooth muscle contraction and thus may be of relevance for airway hyperresponsiveness in asthma. PMID: 27468699
    12. We performed immunohistochemistry for Ki67, p16INK4a, and WNT5A in human HPs ( hyperplastic polyps), sessile serrated adenomas/polyps (SSA/Ps), and traditional serrated adenomas (TSAs) .The distribution of Ki67 and p16INK4a positive cells in TSAs was different from that in HPs and SSA/Ps. PMID: 28627675
    13. Wnt5a-Ror2 signaling enhanced tongue SCC cell aggressiveness and promoted production of MMP-2 following DeltaNp63beta-mediated EMT PMID: 28559016
    14. Here, the s define WNT5A, a non-canonical Wnt ligand implicated in epithelial differentiation, repair, and cancer, as a direct transcriptional target that is activated by KLF4 in squamous epithelial cells. PMID: 27184424
    15. These findings support that Wnt5a-Ror2 signaling plays a role in UC, support the potential use of Wnt5a as a prognostic marker and provide evidence that Wnt5a signaling may be used as an effective molecular target for novel therapeutic tools. PMID: 28427201
    16. We show that rosiglitazone increases klotho and decreases Wnt5A in tumor cells, reducing the burden of both BRAF inhibitor-sensitive and BRAF inhibitor-resistant tumors in aged, but not young mice. However, when used in combination with PLX4720, tumor burden was reduced in both young and aged mice, even in resistant tumors PMID: 28232477
    17. Serum Wnt5a is elevated and associated with disease severity in heart failure patients. PMID: 28357477
    18. In squamous/adenosquamous carcinoma and adenocarcinoma of the gallbladder positive ROR2 or WNT5a expression is generally associated with a poor prognosis. PMID: 28465645
    19. The Wnt5a and its signaling pathway can regulate fundamental cellular processes, including specification of cell fate, proliferation, and survival. PMID: 28641961
    20. Results show that PTEN and WNT5A expression are directly repressed by miR-26b which promotes colorectal cancer metastasis. PMID: 29160937
    21. The relationship between Wnt5a protein expression and histone H4K20me1 was analyzed. Recruitment of H4K20me1 and SET8 to the Wnt5a promoter and coding regions wa investigated. Results demonstrated that the expression levels of Wnt antagonists were generally low in acute myeloid leukemia (AML), but showed differential expression in acute lymphocytic leukemia (ALL). PMID: 28440495
    22. WNT5A and IL-6 are connected through a positive feedback loop in melanoma cells PMID: 27191257
    23. this study reveals that 14-3-3zeta plays a critical role in Wnt5a/ROR1 signaling, leading to enhanced CLL migration and proliferation. PMID: 28465528
    24. these studies indicate HS1 plays an important role in ROR1-dependent Wnt5a-enhanced chemokine-directed leukemia-cell migration. PMID: 28465529
    25. Study reports that WNT5A bi-directionally regulates epithelial-mesenchymal transition (EMT) in mammary epithelial cells, thereby affecting their migration and invasion. However, the ability of WNT5A to inhibit breast cancer cell migration and invasion is an EMT-independent mechanism that, at least in part, can be explained by decreased CD44 expression. PMID: 27623766
    26. disruption of trans-spliced noncoding RNA RMST expression in human embryonic stem cells results in the upregulation of WNT5A, epithelial-to-mesenchymal transition, and lineage-specific genes/markers. PMID: 27090862
    27. Wnt5a suppressed osteoblastic differentiation through Ror2/JNK signaling in periodontal ligament stem cell-like cells. PMID: 28681925
    28. Genetic blockade of autophagy indicated an unexpected feedback loop whereby knocking down the autophagy factor ATG5 in Wnt5A(high) cells decreased Wnt5A and increased beta-catenin. PMID: 28887323
    29. Low WNT5A expression is associated with prostate cancer. PMID: 28748258
    30. WNT5A signaling regulates 15-PGDH expression. PMID: 27522468
    31. our study showed that, for the first time, different Wnt5a mRNA isoforms play distinct roles in colorectal cancer (CRC) and can be used as novel prognostic markers for CRC in the future. PMID: 28859077
    32. High WNT5a expression is associated with osteoarthritis. PMID: 28777797
    33. We conclude that in RS, WNT5A missense mutations have dominant neomorphic effects that interfere with the function of the wild-type protein. PMID: 28662348
    34. RSPO2 suppresses colorectal cancer metastasis by counteracting the Wnt5a/Fzd7-driven noncanonical Wnt pathway. PMID: 28600110
    35. High expression of WNT5A is associated with squamous cell lung carcinoma. PMID: 27876017
    36. Study shows that WNT5A stimulates dimerization of membrane-anchored FZD4 CRDs and oligomerization of full-length FZD4, which requires the integrity of CRD palmitoleoyl-binding residues. These results suggest that FZD receptors may form signalosomes in response to Wnt binding through the CRDs and that the Wnt palmitoleoyl group is important in promoting these interactions. PMID: 28546512
    37. Taken together with our previous findings, we have replicated our results from the rodent system in a novel human system. We have revealed a unique sequential cascade involving Atg10, Wnt5a, alpha1 integrin, and matrix metalloproteinase-3 in GS/BMP-4-induced differentiation of hiPS cells into odontoblast-like cells at a relatively early stage. PMID: 27639333
    38. Elevated WNT5A expression in obesity may function as a negative regulator of angiogenesis.NEW & NOTEWORTHY Wingless-related integration site 5a (WNT5A) negatively regulates adipose tissue angiogenesis via VEGF-A165b in human obesity. PMID: 28411232
    39. Wnt5a as a master regulator of brain invasion, specifically tumor-promoting stem-like characteristics (TPC), and they provide a therapeutic rationale to target it in patients with glioblastoma. PMID: 28011620
    40. inhibition of WNT-5A in vivo attenuated lung tissue destruction, improved lung function, and restored expression of beta-catenin-driven target genes and alveolar epithelial cell markers in the elastase, as well as in cigarette smoke induced models of COPD. PMID: 27979969
    41. Wnt5A/Ryk signaling might provide novel therapeutic strategies to prevent capillary leakage in systemic inflammation and septic shock. PMID: 27159116
    42. Profound vascular insulin resistance in the visceral adipose tissue arterioles of obese subjects was associated with up-regulated WNT5A-JNK signaling and impaired endothelial eNOS activation. PMID: 27688298
    43. Aberrant activation of WNT pathways in patients with ESRD significantly correlated with vascular calcification. PMID: 27156072
    44. Wnt5A could be a potential therapeutic target for reducing microvascular leakage and edema formation in Th2 driven inflammatory diseases. PMID: 27214384
    45. WNT5A and ROR2 are induced by inflammatory mediators through NF-kB and STAT3 transcription factors, and are involved in the migration of human ovarian cancer cell line SKOV-3. PMID: 28536612
    46. decreased UHRF1 expression is a key initial event in the suppression of DNMT1-mediated DNA methylation and in the consequent induction of senescence via increasing WNT5A expression PMID: 28100769
    47. TrpC5 causes a robust rise in [Ca2+]i, enhanced Wnt5a expression and nuclear translocation of beta-catenin, leading to reduced differentiation and enhanced cancer cell stemness. PMID: 27895148
    48. This study describes the localization and functional role of WNT-5A in human and mouse fibrotic livers. Hepatic WNT-5A expression parallels collagen type I expression. In vivo and in vitro, the myofibroblasts were identified as the key hepatic cells producing WNT-5A. WNT-5A is under control of TGF-beta and its activities are primarily profibrotic. PMID: 28057611
    49. this study shows that Wnt5a promotes differentiation of dendritic cells, but inhibits their maturation PMID: 27641635
    50. we confirmed the requirement of Wnt5a in the deferoxamine-mediated osteoblast-promoting effects by analyzing the matrix mineralization of Wnt5a-deficient cells. The promoting effect of deferoxamine on matrix mineralization in wild-type cells was completely abolished in Wnt5a(-/-) cells. PMID: 27540134

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  • 相关疾病：
    Robinow syndrome, autosomal dominant 1 (DRS1)
  • 亚细胞定位：
    Secreted, extracellular space, extracellular matrix. Secreted.
  • 蛋白家族：
    Wnt family
  • 组织特异性：
    Expression is increased in differentiated thyroid carcinomas compared to normal thyroid tissue and anaplastic thyroid tumors where expression is low or undetectable. Expression is found in thyrocytes but not in stromal cells (at protein level). Detected i
  • 数据库链接：

    HGNC: 12784

    OMIM: 164975

    KEGG: hsa:7474

    STRING: 9606.ENSP00000264634

    UniGene: Hs.643085