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  4. Recombinant Human Protein Wnt-7a (WNT7A)

Recombinant Human Protein Wnt-7a (WNT7A)

  • 货号：
    CSB-YP026141HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP026141HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP026141HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP026141HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP026141HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Protein Wnt-7a; Protein Wnt-7a precursor; Proto oncogene Wnt7a protein; proto-oncogene wnt7a protein; wingless-type MMTV integration site family; member 7A; Wnt family member 7A; WNT7A; WNT7A_HUMAN
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    32-349
  • 氨基酸序列
    LGASIICNK IPGLAPRQRA ICQSRPDAII VIGEGSQMGL DECQFQFRNG RWNCSALGER TVFGKELKVG SREAAFTYAI IAAGVAHAIT AACTQGNLSD CGCDKEKQGQ YHRDEGWKWG GCSADIRYGI GFAKVFVDAR EIKQNARTLM NLHNNEAGRK ILEENMKLEC KCHGVSGSCT TKTCWTTLPQ FRELGYVLKD KYNEAVHVEP VRASRNKRPT FLKIKKPLSY RKPMDTDLVY IEKSPNYCEE DPVTGSVGTQ GRACNKTAPQ ASGCDLMCCG RGYNTHQYAR VWQCNCKFHW CCYVKCNTCS ERTEMYTCK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

问答及客户评论

■ 常见问题解答
Q:

Is it possible to produce as a custom product the protein 500µg maximum of 50% glycerol without urea ?

A:
Thanks for your inquiry. Yes. We could provide the protein with size 500µg, and the maximum glycerol concentration is 50%, without urea.

靶点详情

  • 功能：
    Ligand for members of the frizzled family of seven transmembrane receptors that functions in the canonical Wnt/beta-catenin signaling pathway. Plays an important role in embryonic development, including dorsal versus ventral patterning during limb development, skeleton development and urogenital tract development. Required for central nervous system (CNS) angiogenesis and blood-brain barrier regulation. Required for normal, sexually dimorphic development of the Mullerian ducts, and for normal fertility in both sexes. Required for normal neural stem cell proliferation in the hippocampus dentate gyrus. Required for normal progress through the cell cycle in neural progenitor cells, for self-renewal of neural stem cells, and for normal neuronal differentiation and maturation. Promotes formation of synapses via its interaction with FZD5.
  • 基因功能参考文献：
    1. These results indicate that Wnt7a signaling inhibits inflammatory stimuli-induced catabolic gene expression in human articular chondrocytes and is sufficient to attenuate MMP activities and promote joint cartilage integrity in mouse experimental OA, demonstrating a novel effect of Wnt7a on regulating OA pathogenesis. PMID: 28165497
    2. Wnt7a induces a unique phenotype of monocyte-derived macrophages with lower phagocytic capacity and differential expression of pro- and anti-inflammatory cytokines PMID: 28872671
    3. This report deals with linkage studies and exome sequencing, disclosing a novel variant in WNT7A, c.934G>A (p.Gly312Ser), as the cause of this Santos syndrome. PMID: 28855715
    4. A homozygous novel WNT7A mutation was identified in a child with Al-Awadi-Raas-Rothschild syndrome accompanied by dental abnormalities and his parents with dental abnormalities. PMID: 28917830
    5. Activation of the WNT/CTNBB1 pathway via WNT7A might play a role in PTC development. PMID: 28456695
    6. Results show that Wnt7a expression is significantly upregulated in epithelial cells of ovarian cancer which correlates with STAT4 expression and provide evidence that STAT4 promotes ovarian cancer metastasis via tumor-derived Wnt7a-induced activation of cancer-associated fibroblasts. PMID: 28114283
    7. WNT7A is a direct target of miR-15b in ovarian cancer cell line. PMID: 27195958
    8. The findings presented in this fetus are compatible with diagnosis of Al-Awadi-Raas-Rothschild syndrome , expanding the mutational spectrum of limb malformations arising from defects in WNT7A PMID: 27638328
    9. Wnt7a is involved in the transformation of the retinal pigment epithelium. PMID: 26388562
    10. Wnt7a overexpression is associated with an unfavorable prognosis and that positive Wnt7a may be an independent prognosis factor influencing OS and DFS prediction in colorectal cancer patients. PMID: 26055144
    11. identified Iloprost, a prostacyclin analog, which initiates downstream signaling cascades similar to that of Wnt7a, as a novel inducer of cellular senescence, presenting potential future clinical translational strategies PMID: 25728679
    12. Prognosis was significantly more favorable for patients with high Wnt7A expression. PMID: 25632963
    13. loss of the Wnt7a gene induced by promoter methylation might be a prognostic factor for non-small cell lung carcinoma PMID: 25653486
    14. this is the first study reporting reduced WNT7A levels in cervical cancer -derived cells and that ectopic WNT7A restoration negatively affects cell proliferation and migration. PMID: 25978974
    15. Wnt7a has a role in specifying the fate of neural crest-like cells via suppression of notch in the human skin microenvironment PMID: 25705850
    16. findings suggest a central role of the WNT7A-PAX6 axis in corneal epithelial cell fate determination, and point to a new strategy for treating corneal surface diseases PMID: 25030175
    17. Binding of Wnt7a to Fzd7 leads to an activation of noncanonical Wnt signaling, resulting in directed myogenic stem cell migration and enhanced engraftment. PMID: 24711502
    18. Novel mutations in the WNT7A gene in two unrelated cases of Al-Awadi Raas-Rothschild syndrome. PMID: 23727605
    19. Gene expression profiling identifies WNT7A as a key SVZ-glial factor lacking in OB-glia that enhances self-renewal, thereby improving the propagation of OB-NSC cultures. PMID: 22987443
    20. Overexpression of Wnt7a may contribute to the progression of endometrial cancer and thus may serve as a new biomarker to predict the prognosis of endometrial cancer. PMID: 23321718
    21. WNT7A gene is inactivated by genetic/epigenetic alterations in clear cell RCC and demonstrates tumor suppressor properties PMID: 23056560
    22. Wnt7a is lost by methylation in a subset of tumors and that this methylation is maintained by DNMT1 PMID: 22403725
    23. Reexpression of WNT7A during malignant transformation of ovarian epithelial cells plays a critical role in ovarian cancer progression mediated by WNT/beta-catenin signaling pathway. PMID: 22232518
    24. Wnt7a has a role in postmenstrual regeneration and proliferation of endometrial glands and luminal epithelium in primates PMID: 22294752
    25. Mutations in the coding sequence of WNT7A are not responsible for mullerian duct abnormalities in the Chinese population. PMID: 22177312
    26. The novel missense homozygous mutation (p.Gly204Ser) in the WNT7A gene is a unique mutation in the degree of loss of function in the upper limb development which ranges from mild to complete absence of both upper limbs (amelia). PMID: 21344627
    27. Partial loss of WNT7A function resulted in Fuhrmann syndrome, while complete loss of WNT7A function resulted in the more severe phenotype of Al-Awadi-Raas-Rothschild syndrome. PMID: 21271649
    28. Abnormalities in the Wnt7a pathway (located in the dorsal ectoderm) produce several clinically relevant conditions. PMID: 20709709
    29. A novel homozygous missense mutation in coding exon 4 of the WNT7A was detected in both affected daughters (c.664C > T) leading to an amino acid exchange from arginine to tryptophan (p.Arg222Trp; R222W). PMID: 20949531
    30. The activity of the Sprouty4 promoter is increased by Wnt7A/Frizzled 9 homolog (Fzd9) signaling through peroxisome proliferator-activated receptor gamma in lung cancer cells. PMID: 20501643
    31. High Wnt7a expression in ovarian cancer may be associated with poor prognosis. PMID: 20845993
    32. Expression levels of Wnt7A, beta-catenin, and FoxA1, along with cell-type specific markers, are observed to vary with differentiation and often also in response to the presence of heparin during the time of exposure to heparin. PMID: 20503388
    33. Results suggest that this diminished expression of the WNT7A gene may be related to a supposed protection of fragile X syndrome patients to develop cancer. PMID: 20470940
    34. Female mammals with a deficiency in the product of Wnt7a gene are infertile as a result of abnormal development of the uterus and the oviducts. PMID: 19849868
    35. WNT7a gene expression is regulated by TGF-beta1 through the intracellular beta-catenin-TCF pathway PMID: 12893825
    36. provides support that E-cadherin induction by WNT/beta-catenin signaling is an evolutionarily conserved pathway operative in lung cancer cells and that loss of expression may be important in lung cancer development or progression PMID: 12937339
    37. WNT7A mutations are an unlikely cause of Mullerian duct derivative abnormalities in humans PMID: 14550385
    38. Wnt-7a is associated with cartilage destruction by regulating the maintenance of differentiation status and the apoptosis of articular chondrocytes via different mechanisms PMID: 15082716
    39. analysis of multiple Wnt mRNAs in non-small cell lung cancer (NSCLC) cell lines and primary lung tumors revealed markedly decreased Wnt-7a expression compared with normal short-term bronchial epithelial cell lines and normal uninvolved lung tissue PMID: 15705594
    40. By regulating the proliferation of corneal epithelial cells, Wnt-7a and MMP-12 appear to contribute to corneal wound healing PMID: 15802269
    41. Partial loss of WNT7A causes Fuhrmann syndrome, whereas the more severe limb truncation phenotypes observed in Al-Awadi/Raas-Rothschild/Schinzel phocomelia syndrome result from null mutations. PMID: 16826533
    42. ERK5-dependent activation of PPARgamma represents a major effector pathway mediating the anti-tumorigenic effects of Wnt 7a and Fzd 9 in non-small cell lung cancer cells PMID: 16835228
    43. the absence of tumour-specific somatic events in WNT7A and HDAC11 suggests that these genes are unlikely to have a classical tumour suppressor gene role in sporadic malignant pancreatic endocrine tumours PMID: 17201809
    44. WNT7A plays a role in the pathophysiology of endometriosis. PMID: 17588571
    45. These biological tools could help lead to a better understanding of Wnt-Fzd interactions and the identification of new modulators of Wnt signaling. PMID: 18230341
    46. WNT7A gene is unlikely to be a major contributor to the aetiology of familial congenital talipes equinovarus PMID: 18538017
    47. Based on these results it is unlikely CAND2 and WNT7a are the major genes that causes clubfoot. PMID: 19159115

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  • 相关疾病：
    Limb pelvis hypoplasia aplasia syndrome (LPHAS); Fuhrmann syndrome (FUHRS)
  • 亚细胞定位：
    Secreted, extracellular space, extracellular matrix. Secreted.
  • 蛋白家族：
    Wnt family
  • 组织特异性：
    Expression is restricted to placenta, kidney, testis, uterus, fetal lung, and fetal and adult brain.
  • 数据库链接：

    HGNC: 12786

    OMIM: 228930

    KEGG: hsa:7476

    STRING: 9606.ENSP00000285018

    UniGene: Hs.72290