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  4. Recombinant Human Protein arginine N-methyltransferase 2 (PRMT2)

Recombinant Human Protein arginine N-methyltransferase 2 (PRMT2)

  • 中文名称：
    人PRMT2重组蛋白
  • 货号：
    CSB-YP018732HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人PRMT2重组蛋白
  • 货号：
    CSB-EP018732HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人PRMT2重组蛋白
  • 货号：
    CSB-EP018732HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人PRMT2重组蛋白
  • 货号：
    CSB-BP018732HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人PRMT2重组蛋白
  • 货号：
    CSB-MP018732HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    PRMT2
  • Uniprot No.：
  • 别名：
    ANM2_HUMAN; EC 2.1.1.; histone arginine N methyltransferase PRMT2; Histone-arginine N-methyltransferase PRMT2; HMT 1; HMT1 (hnRNP methyltransferase S. cerevisiae) like 1; HMT1; HMT1 hnRNP methyltransferase like 1; hnRNP methyltransferase (S. cerevisiae) like 1; hnRNP methyltransferase like 1; Hrmt1l1; MGC111373; PRMT 2; PRMT2 alpha; prmt2; PRMT2 beta; PRMT2 gamma; PRMT2 protein; PRMT2L2; Protein arginine methyltransferase 2; Protein arginine N methyltransferase 2; Protein arginine N-methyltransferase 2; Zf2
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-433
  • 氨基酸序列
    MATSGDCPRS ESQGEEPAEC SEAGLLQEGV QPEEFVAIAD YAATDETQLS FLRGEKILIL RQTTADWWWG ERAGCCGYIP ANHVGKHVDE YDPEDTWQDE EYFGSYGTLK LHLEMLADQP RTTKYHSVIL QNKESLTDKV ILDVGCGTGI ISLFCAHYAR PRAVYAVEAS EMAQHTGQLV LQNGFADIIT VYQQKVEDVV LPEKVDVLVS EWMGTCLLFE FMIESILYAR DAWLKEDGVI WPTMAALHLV PCSADKDYRS KVLFWDNAYE FNLSALKSLA VKEFFSKPKY NHILKPEDCL SEPCTILQLD MRTVQISDLE TLRGELRFDI RKAGTLHGFT AWFSVHFQSL QEGQPPQVLS TGPFHPTTHW KQTLFMMDDP VPVHTGDVVT GSVVLQRNPV WRRHMSVALS WAVTSRQDPT SQKVGEKVFP IWR
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Arginine methyltransferase that methylates the guanidino nitrogens of arginyl residues in proteins such as STAT3, FBL, histone H4. Acts as a coactivator (with NCOA2) of the androgen receptor (AR)-mediated transactivation. Acts as a coactivator (with estrogen) of estrogen receptor (ER)-mediated transactivation. Enhances PGR, PPARG, RARA-mediated transactivation. May inhibit NF-kappa-B transcription and promote apoptosis. Represses E2F1 transcriptional activity (in a RB1-dependent manner). May be involved in growth regulation.
  • 基因功能参考文献：
    1. Results show that tamoxifen resistance in breast cancer cells is connected with the downregulation of PRMT2 and the upregulation of ER-alpha36. Moreover, PRMT2 was able to interact with ER-alpha36 directly, suppress ER-alpha36 and downstream PI3K/Akt and MAPK/ERK signaling, reversing the tamoxifen resistance of breast cancer cells. PMID: 29620287
    2. Low splice variant of PRMT2 expression is associated with breast cancer. PMID: 28677794
    3. Cobl-mediated dendritic arborization required PRMT2, complex formation with PRMT2, and PRMT2's catalytic activity PMID: 29689199
    4. The PRMT2 interacts with SAM68 in cells and regulates its subcellular localization via the SH3 domain of PRMT2, prompting us to investigate the potential role of PRMT2 in BCL-X alternative splicing. PMID: 28057797
    5. This study demonstrates the role and significant correlation between a histone methyltransferase (PRMT2)-dependent signature, RORgamma, the cell-cycle regulation, DNA repair circuits, and breast cancer survival outcomes. PMID: 24911119
    6. This study uncovers the molecular mechanism of PRMT2 regulating the expression of CCND1 in invasive ductal breast carcinoma. PMID: 24292672
    7. characterization of 3 PRMT2 splice variants (PRMT2alpha, PRMT2beta, and PRMT2gamma) from breast cancer; these variants bind to ERalpha in vitro/in vivo; overall PRMT2 expression is upregulated in breast cancer and associated w/ ERalpha positivity PMID: 22093364
    8. Data suggest that post-transcriptional processing mechanism as alternative polyadenylation and splicing may play a crucial role in regulating human PRMT2 gene expression. PMID: 21820040
    9. the SH3 domain may mediate an interaction between PRMT1 and -2 in a methylation-dependent fashion PMID: 21851090
    10. PRMT2 is a novel ERalpha coactivator. PMID: 12039952
    11. PRMT2 inhibits NF-kappa B function and promotes apoptosis. PMID: 16648481
    12. ligand-dependent AR conformation is essential for the recruitment and nuclear translocation of PMRT2 which acts as AR-coactivator, presumably by arginine methylation. PMID: 17587566
    13. Here we report methylation activity from PRMT2 and compare it with PRMT1 activity using UPLC-MS/MS (ultra-performance liquid chromatography-tandem MS), gel electrophoresis, and thin-layer chromatography PMID: 19405910

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  • 亚细胞定位：
    [Isoform 1]: Cytoplasm. Nucleus. Note=Translocates from the cytoplasm to the nucleus, after hormone exposure. Excluded from nucleolus.; [Isoform PRMT2Alpha]: Nucleus. Note=Excluded from nucleolus.; [Isoform PRMT2Beta]: Cytoplasm. Nucleus. Nucleus, nucleolus.; [Isoform PRMT2Gamma]: Nucleus. Note=Excluded from nucleolus.; [Isoform PRMT2L2]: Cytoplasm. Nucleus. Note=Predominantly cytoplasmic.
  • 蛋白家族：
    Class I-like SAM-binding methyltransferase superfamily, Protein arginine N-methyltransferase family
  • 组织特异性：
    Widely expressed. Highly expressed in androgen target organs such as heart, prostate, skeletal muscle, ovary and spinal cord.
  • 数据库链接：

    HGNC: 5186

    OMIM: 601961

    KEGG: hsa:3275

    STRING: 9606.ENSP00000347906

    UniGene: Hs.154163