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  4. Recombinant Human Protein-glutamine gamma-glutamyltransferase E (TGM3)

Recombinant Human Protein-glutamine gamma-glutamyltransferase E (TGM3)

  • 中文名称：
    人TGM3重组蛋白
  • 货号：
    CSB-YP600462HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人TGM3重组蛋白
  • 货号：
    CSB-EP600462HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人TGM3重组蛋白
  • 货号：
    CSB-EP600462HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人TGM3重组蛋白
  • 货号：
    CSB-BP600462HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人TGM3重组蛋白
  • 货号：
    CSB-MP600462HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    TGM3
  • Uniprot No.：
  • 别名：
    E polypeptide protein glutamine gamma glutamyltransferase; MGC126249; MGC126250; Protein glutamine gamma glutamyltransferase E 27 kDa catalytic chain; Protein glutamine gamma glutamyltransferase E 50 kDa noncatalytic chain; Protein glutamine gamma glutamyltransferase E; Protein-glutamine gamma-glutamyltransferase E 27 kDa non-catalytic chain; TG(E); TGase E; TGase-3; TGE; TGM 3; TGM3; TGM3_HUMAN; Transglutaminase E; Transglutaminase-3; Transglutaminase3; TransglutaminaseE
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-467
  • 氨基酸序列
    AALGVQSIN WQTAFNRQAH HTDKFSSQEL ILRRGQNFQV LMIMNKGLGS NERLEFIVST GPYPSESAMT KAVFPLSNGS SGGWSAVLQA SNGNTLTISI SSPASAPIGR YTMALQIFSQ GGISSVKLGT FILLFNPWLN VDSVFMGNHA EREEYVQEDA GIIFVGSTNR IGMIGWNFGQ FEEDILSICL SILDRSLNFR RDAATDVASR NDPKYVGRVL SAMINSNDDN GVLAGNWSGT YTGGRDPRSW NGSVEILKNW KKSGFSPVRY GQCWVFAGTL NTALRSLGIP SRVITNFNSA HDTDRNLSVD VYYDPMGNPL DKGSDSVWNF HVWNEGWFVR SDLGPSYGGW QVLDATPQER SQGVFQCGPA SVIGVREGDV QLNFDMPFIF AEVNADRITW LYDNTTGKQW KNSVNSHTIG RYISTKAVGS NARMDVTDKY KYPEGSDQER QVFQKALGKL KPNTPFA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the calcium-dependent formation of isopeptide cross-links between glutamine and lysine residues in various proteins, as well as the conjugation of polyamines to proteins. Involved in the formation of the cornified envelope (CE), a specialized component consisting of covalent cross-links of proteins beneath the plasma membrane of terminally differentiated keratinocytes. Catalyzes small proline-rich proteins (SPRR1 and SPRR2) and LOR cross-linking to form small interchain oligomers, which are further cross-linked by TGM1 onto the growing CE scaffold. In hair follicles, involved in cross-linking structural proteins to hardening the inner root sheath.
  • 基因功能参考文献：
    1. Patents having higher level of TGM-3 expression have good response to chemo-radiotherapy and also have better overall survival. TGM-3 may serve as a candidate biomarker for responsiveness to chemo-radiotherapy treatment in oral squamous cell carcinoma patients PMID: 29953521
    2. Cultured duodenal biopsies from patients with dermatitis herpetiformis produce transglutaminase-3 antibodies. PMID: 29182792
    3. Levels of TG3-IgA IC significantly elevated in plasma as well as in serum samples of untreated patients with dermatitis herpetiformis compared with healthy controls. PMID: 27106676
    4. studies demonstrate that TGase-catalyzed transamidation and activation of rac1 and cdc42 results from stimulation of multiple types of receptors and this novel signaling pathway can regulate dendritic spine morphology and plasticity. PMID: 28161375
    5. The two enzymes PADI3 and TGM3, responsible for posttranslational protein modifications, and their target structural protein TCHH are all involved in hair shaft formation. PMID: 27866708
    6. Our data collectively demonstrate that TGM3 can be a candidate tumor suppressor that is able to induce esophageal cancer cell proliferation and migration PMID: 27430245
    7. Transglutaminase 3 present in the IgA aggregates in dermatitis herpetiformis skin is enzymatically active and binds soluble fibrinogen. PMID: 25178107
    8. New basal cell carcinoma susceptibility loci were identified at TGM3 (rs214782) and RGS22(rs7006527). PMID: 24403052
    9. TGM3, a candidate tumor suppressor, contributes to the carcinogenesis and development of human head and neck cancer. PMID: 24289313
    10. The low expression of TGM3 may contribute to the carcinogenesis and development of laryngeal carcinoma. PMID: 22506423
    11. TGM3 in immunoglobulin A (IgA) and TGM3 immune complexes is responsible for cross-linking and tight binding of IgA to connective tissue in the dermis and explains the IgA deposits in dermatitis herpetiformis skin long after signs of disease are resolved. PMID: 21335491
    12. findings lend credence to the notion that TG3 and TG6 are involved in the gluten-induced autoimmune responses of dermatitis herpetiformis and gluten ataxia PMID: 20300788
    13. new information on the specific distribution of TGase 3 PMID: 20716179
    14. epidermal transglutaminase (type 3) is the dominant autoantigen in dermatitis herpetiformis PMID: 11901200
    15. x-ray crystallography in the presence of Ca2+ and/or Mg+2 shows Ca2+ binding in both sites two and three cooperate in opening the channel PMID: 12679341
    16. Expression in upper layers of epidermis. Diffuse cytoplasmic distribution in vitro consistent with its proposed role in early phase of cornified cell envelope assembly in cytoplasm. PMID: 12850301
    17. transglutaminase 3 is regulated by guanine nucleotides and calcium/magnesium PMID: 14645372
    18. Crystal structure of transglutaminase 3 in complex with GMP PMID: 15084592
    19. upstream sequence of Ets core motif is critical for the expression of TGM3 in cells cultured in vitro. PMID: 15335352
    20. In HEK 293T cell culture, transglutaminase 3 cross-links the N-terminal fragments of mutant huntingtin protein, therefore it could be involved in the cross-linking of huntingtin into intranuclear inclusions in Huntington disease. PMID: 15715085
    21. We conclude from these in vitro experiments that the E1--E4 protein is cross-linked by transglutaminase 3, and that E1--E4 expression in differentiated keratinocytes induces morphologic abnormalities of the cornified cell envelope. PMID: 16257432
    22. Reduced expression of transglutaminase 3 (TGM3) may play an important role in esophageal carcinogenesis. PMID: 16804985
    23. Using a new antibody raised in goat with specificity confirmed using a separately cloned recombinant protein, we have confirmed findings of Sardy et al. suggesting TG3 is the autoantigen of dermatitis herpetiformis PMID: 17205060
    24. detected two splicing variants for TG3, as the transcription of these mRNAs appears to be not regulated during differentiation and in pathologies involving tranglutaminase activity PMID: 17380116
    25. TGase 3-driven specific isopeptide bonds between intermediate filaments and keratin associated proteins participate to the progressive scaffolding of the hair shaft PMID: 18719606
    26. Cathepsin L and transglutaminase 3 could be involved in the pathway that leads to terminal differentiation, not only in the epidermis but also in the human hair follicle and nail unit. PMID: 19005484
    27. Reduced TGM3 expression is associated with esophageal squamous cell carcinoma. PMID: 19142970
    28. Lack of TGM3 expression is associated with oral squamous cell carcinoma. PMID: 19522851

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  • 相关疾病：
    Uncombable hair syndrome 2 (UHS2)
  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Transglutaminase superfamily, Transglutaminase family
  • 数据库链接：

    HGNC: 11779

    OMIM: 600238

    KEGG: hsa:7053

    STRING: 9606.ENSP00000370867

    UniGene: Hs.2022