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  4. Recombinant Human Protein quaking (QKI)

Recombinant Human Protein quaking (QKI)

  • 中文名称：
    人QKI重组蛋白
  • 货号：
    CSB-EP842739HU
  • 规格：
    ￥1344
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    QKI
  • Uniprot No.：
  • 别名：
    DKFZp586I0923; HKQ; Homolog of mouse quaking QKI KH domain RNA binding protein; Hqk; HQK1; HqkI; OTTHUMP00000017581; OTTHUMP00000017582; OTTHUMP00000017583; Protein quaking; QK; QK1; QK3; QKI; QKI_HUMAN; QKI1; Quaking homolog; Quaking homolog KH domain RNA binding; Quaking homolog KH domain RNA binding mouse; Quaking isoform 1; Quaking protein; RNA binding protein HQK
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length
  • 来源：
    E.coli
  • 分子量：
    64.7kDa
  • 表达区域：
    1-341aa
  • 氨基酸序列
    MVGEMETKEKPKPTPDYLMQLMNDKKLMSSLPNFCGIFNHLERLLDEEISRVRKDMYNDTLNGSTEKRSAELPDAVGPIVQLQEKLYVPVKEYPDFNFVGRILGPRGLTAKQLEAETGCKIMVRGKGSMRDKKKEEQNRGKPNWEHLNEDLHVLITVEDAQNRAEIKLKRAVEEVKKLLVPAAEGEDSLKKMQLMELAILNGTYRDANIKSPALAFSLAATAQAAPRIITGPAPVLPPAALRTPTPAGPTIMPLIRQIQTAVMPNGTPHPTAAIVPPGPEAGLIYTPYEYPYTLAPATSILEYPIEPSGVLGAVATKVRRHDMRVHPYQRIVTADRAATGN
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal GST-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    RNA-binding protein that plays a central role in myelinization. Binds to the 5'-NACUAAY-N(1,20)-UAAY-3' RNA core sequence. Regulates target mRNA stability. In addition, acts by regulating pre-mRNA splicing, mRNA export and protein translation. Required to protect and promote stability of mRNAs such as MBP and CDKN1B. Regulator of oligodendrocyte differentiation and maturation in the brain that may play a role in myelin and oligodendrocyte dysfunction in schizophrenia. Participates in mRNA transport by regulating the nuclear export of MBP mRNA. Also involved in regulation of mRNA splicing of MAG pre-mRNA. Acts as a translational repressor.
  • 基因功能参考文献：
    1. Study examined if QKI6B expression can predict the outcome of GFAP, and several oligodendrocyte-related genes, in the prefrontal cortex of brain samples of schizophrenic individuals. QKI6B significantly predicts the expression of GFAP, but does not predict oligodendrocyte-related gene outcome, as previously seen with other QKI isoforms. PMID: 28552414
    2. The results of this study have found an upregulation of QKI and its isoforms (QKI5, QKI6 and QKI7) in Sporadic Alzheimer's Disease patients compared to controls PMID: 27163826
    3. QKI5 could inhibit gastric cancer cell proliferation, migration, and invasion in vitro and in vivo, which might be due to the altered splicing pattern of macroH2A1 pre-mRNA, leading to the accumulation of macroH2A1.1 isoform. PMID: 27092877
    4. several splicing variants with functional relevance in lung cancer largely regulated by the splicing factor QKI, were identified. PMID: 27555542
    5. QKI underexpression is associated with glioma. PMID: 27841882
    6. Our results demonstrate that miR-143-3p acts as a tumor-suppressor by targeting QKI-5 in ESCC, suggesting that miR-143-3p is a potential therapy for the treatment of ESCC. PMID: 27358073
    7. Methylation of the QKI promoter and concomitant reduced expression of QKI mRNA may be important for CRC initiation and progression. PMID: 28179294
    8. quaking is essential in maintaining endothelial barrier function. PMID: 26905650
    9. QKI-7 recruits PAPD4 to regulate post-transcriptional polyadenylation of target mRNAs. PMID: 26926106
    10. QKI has a role in posttranscriptionally guiding macrophage identity and function. PMID: 27029405
    11. MYB-QKI rearrangements promote tumorigenesis through three mechanisms: MYB activation by truncation, enhancer translocation driving aberrant MYB-QKI expression and hemizygous loss of the tumor suppressor QKI PMID: 26829751
    12. Data implicate QKI in the pathophysiology of inflammation and leukemoogenesis where miR-155 is involved. PMID: 26337206
    13. miR-155 regulates the cell cycle and invasion ability of colon cancer cells via the modulation of QKI expression PMID: 25420938
    14. miR-29a promotes scavenger receptor A expression by targeting QKI during monocyte-macrophage differentiation. PMID: 26056009
    15. QKI is a novel Cancer stem cells (CSC) inhibitor and impaired multiple oral CSC properties via partial repression of SOX2. PMID: 24918581
    16. Using circScreen, study identified the RNA binding protein Quaking (QKI) as a major regulator of circRNA biogenesis in epithelial-mesenchymal transition (EMT) and show that introduction of consensus binding sequences for QKI into the flanking introns is sufficient to cause circRNAs to be produced from exons that normally only undergo canonical linear splicing. PMID: 25768908
    17. QKI as a key regulator of alternative splicing in lung cancer. PMID: 24722255
    18. QKI-mediated repression of FOXO1 may be one of the factors contributing to the oncogenesis and progression of breast carcinoma. PMID: 24398626
    19. the QKI-5 expression may be a novel, independent factor in the prognosis of prostate cancer patients. PMID: 24153116
    20. Propose that QKI is a central regulator of VSMC phenotypic plasticity and that intervention in QKI activity can ameliorate pathogenic, fibroproliferative responses to vascular injury. PMID: 23963726
    21. Data showed that like QKI5/6/7 proteins, QKI7b protein was also significantly downregulated in most human colorectal cancer tissues. PMID: 23440637
    22. QKI is a hub regulator of glia function in humans. PMID: 23321059
    23. This work provides the first evidence that QK is a global regulator of splicing during muscle development in vertebrates and shows how overlapping splicing regulatory networks contribute to gene expression programs during differentiation. PMID: 23525800
    24. Findings suggest that miR-574-5p is a potent ribo-regulator for Qkis and that aberrant miR-574-5p upregulation can be oncogenic. PMID: 22490519
    25. The RNA-binding protein QKI5 is a direct target of C/EBPalpha and delays macrophage differentiation. PMID: 22398723
    26. establish that p53 directly regulates Quaking (QKI) gene expression, and QKI protein associates with and leads to the stabilization of miR-20a PMID: 22751500
    27. Multivariate analysis showed QKI expression was an independent prognostic factor for patient survival. PMID: 22569043
    28. The QKI RNA-binding proteins regulate oligodendrocyte differentiation by modulating the expression of AIP-1. PMID: 20631256
    29. E2F1 directly transcribes QKI, which, in turn, negatively regulates the cell cycle by targeting multiple cell cycle regulators, forming an E2F1-QKI-pRb/E2F1 negative feedback loop. PMID: 21768773
    30. QKI, regulates the alternative splicing of macroH2A1 pre-mRNA, resulting in increased levels of macroH2A1.1. PMID: 21844227
    31. HSV-1 infection promotes quaking RNA binding protein production and induces nuclear-cytoplasmic shuttling of quaking I-5 isoform in human hepatoma cells PMID: 21467216
    32. QKI-7 regulates expression of interferon-related genes in human astrocyte glioma cells. PMID: 20927331
    33. QKI functions as a principal regulator in the differentiation of colon epithelium and a suppressor of carcinogenesis through coordinately targeting multiple genes associated with cell growth and differentiation PMID: 19686745
    34. QKI as a possible target for functional studies related to the role of myelination in schizophrenia. PMID: 16342280
    35. QKI levels may regulate oligodendrocyte differentiation and maturation in human brain PMID: 16641098
    36. Since QKI plays a fundamental role in oligodendrocyte differentiation and in myelination, its underexpression may be pivotal to, and upstream of, other myelin-associated gene expression abnormalities in schizophrenia. PMID: 17012699
    37. QKI-6 is the major protein isoform responsible for central nervous system (CNS) myelination, which promotes myelin basic protein expression in oligodendrocytes. PMID: 17079655
    38. biological functions attributed to this KH-type RNA binding protein and the recent achievements linking it to human disorders PMID: 17787018
    39. we examined expression of QKI and several putative mRNA targets of QKI in human PFC and hippocampus at different ages PMID: 17918747
    40. variants within the promoter region of QKI gene are unlikely to play a major role in susceptibility to schizophrenia in the Chinese population PMID: 18938205
    41. The observation of consistent reductions in multiple isoforms of QKI mRNA in depressed suicide victims supports the growing body of evidence for a role of myelination-related deficits in the etiology of psychiatric disorders PMID: 19545858
    42. There are many isoforms of this RNA binding protein. QKI-6 has been shown to be a translational repressor. PMID: 10535969

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  • 亚细胞定位：
    Nucleus. Cytoplasm.
  • 组织特异性：
    Expressed in the frontal cortex of brain. Down-regulated in the brain of schizophrenic patients.
  • 数据库链接：

    HGNC: 21100

    OMIM: 609590

    KEGG: hsa:9444

    STRING: 9606.ENSP00000355094

    UniGene: Hs.510324