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  4. Recombinant Human Protein regulator of cytokinesis 1 (PRC1)

Recombinant Human Protein regulator of cytokinesis 1 (PRC1)

  • 货号：
    CSB-YP018633HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP018633HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP018633HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP018633HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP018633HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    PRC1
  • Uniprot No.：
  • 别名：
    Anaphase spindle elongation 1 homolog; ASE1; PRC1; PRC1_HUMAN; Protein regulating cytokinesis 1; Protein regulator of cytokinesis 1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-620
  • 氨基酸序列
    MRRSEVLAEE SIVCLQKALN HLREIWELIG IPEDQRLQRT EVVKKHIKEL LDMMIAEEES LKERLIKSIS VCQKELNTLC SELHVEPFQE EGETTILQLE KDLRTQVELM RKQKKERKQE LKLLQEQDQE LCEILCMPHY DIDSASVPSL EELNQFRQHV TTLRETKASR REEFVSIKRQ IILCMEALDH TPDTSFERDV VCEDEDAFCL SLENIATLQK LLRQLEMQKS QNEAVCEGLR TQIRELWDRL QIPEEEREAV ATIMSGSKAK VRKALQLEVD RLEELKMQNM KKVIEAIRVE LVQYWDQCFY SQEQRQAFAP FCAEDYTESL LQLHDAEIVR LKNYYEVHKE LFEGVQKWEE TWRLFLEFER KASDPNRFTN RGGNLLKEEK QRAKLQKMLP KLEEELKARI ELWEQEHSKA FMVNGQKFME YVAEQWEMHR LEKERAKQER QLKNKKQTET EMLYGSAPRT PSKRRGLAPN TPGKARKLNT TTMSNATANS SIRPIFGGTV YHSPVSRLPP SGSKPVAAST CSGKKTPRTG RHGANKENLE LNGSILSGGY PGSAPLQRNF SINSVASTYS EFAKDPSLSD SSTVGLQREL SKASKSDATS GILNSTNIQS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Key regulator of cytokinesis that cross-links antiparrallel microtubules at an average distance of 35 nM. Essential for controlling the spatiotemporal formation of the midzone and successful cytokinesis. Required for KIF14 localization to the central spindle and midbody. Required to recruit PLK1 to the spindle. Stimulates PLK1 phosphorylation of RACGAP1 to allow recruitment of ECT2 to the central spindle. Acts as an oncogene for promoting bladder cancer cells proliferation, apoptosis inhibition and carcinogenic progression.
  • 基因功能参考文献：
    1. PRC1 was identified as a type 2 diabetes susceptibility gene. PRC1 plays a role in insulin secretion in vitro. PMID: 28580277
    2. We discuss targeting PRC1 within the complementary approaches of either normalizing chromosomal instability in aneuploid cancers or creating chromosomal chaos in genomically stable cancers to induce apoptosis. PMID: 29413422
    3. PRC1 mRNA and protein expressions were upregulated in lung adenocarcinoma tissues. PRC1 expression was significantly correlated with the Wnt/beta-catenin signaling pathway. PMID: 28646916
    4. We finally confirmed that PRC1 is a novel downstream target of piperlongumine in gastric cancer. Our findings supported the oncogenic role of PRC1 in gastric carcinogenesis. PRC1 might serve as a prognostic biomarker and potential therapeutic target for gastric carcinoma. PMID: 28190297
    5. These findings suggest that the identified APLP2, RRM2, and PRC1 signature could be useful for distinguishing between benign (follicular adenoma) and malignant (follicular carcionma and follicular variant of papillary carcinoma) tumors of the thyroid follicular epithelium. PMID: 27796194
    6. Near-atomic cryo-electron microscopy structure of PRC1 bound to the microtubule has been described. PMID: 27493215
    7. PRC1 is a novel Wnt target that functions in a positive feedback loop that reinforces Wnt signalling to promote early Hepatocellular Carcinoma recurrence. PMID: 26941395
    8. PRC1 rs10520699 and rs11852999 polymorphisms were associated with PRC1 transcript levels, but not with patients survival. PMID: 27505863
    9. These findings suggest that the aberrant expression of PRC1 may predict biochemical recurrence in men with prostate cancer highlighting its potential as a prognostic marker of this malignancy. PMID: 26898432
    10. PP2A-B55 dephosphorylates PRC1 at the metaphase to anaphase transition. This activity is regulated by the Greatwall (MASTL) kinase and its substrate ENSA, explaining the timing of PRC1 activation in anaphase. PMID: 24120663
    11. PRC1 complexes isolated from human cells contain multiple kinesins KIF4A, Kif20A, KIF23 and KIF14. PMID: 16431929
    12. PRC1 is a docking partner for the Polo kinase Plk1 in anaphase cells. During metaphase this is inhibited by Cdk1-cyclin B phosphorylation at T470 and T481. PMID: 17351640
    13. The presence of CBX4 or CBX8-GFP in the same focus had a minor impact on BMI1 and RING1 recovery kinetics. PMID: 24460908
    14. Study finds that frictional forces increase nonlinearly with microtubule-associated proteins (MAP) velocity across microtubules and depend on filament polarity, with NuMA's friction being lower when moving toward minus ends, EB1's lower toward plus ends, and PRC1's exhibiting no directional preference. PMID: 24725408
    15. Studies reveal how interactions between the conserved nonmotor MAP, PRC1, and the motor protein, kinesin-4, generate filament length-dependent tags at microtubule plus ends. PRC1 tags ends of microtubules in dividing cells and the size of these tags increases linearly with filament length. PMID: 23870126
    16. Different PRC1 paralog family members have nonredundant and locus-specific gene regulatory activities that are essential for human hematopoiesis. PMID: 23349393
    17. findings suggest that PRC1 is regulated by Plk1, rather than Cdk1 as previously proposed, because its activity must be spatiotemporally regulated both preanaphase and postanaphase, and Cdk1 activity is too binary for this purpose PMID: 22621898
    18. Two distinct mechanisms are involved in CBX2-mediated gene silencing. The short CBX2-2 isoform would repress the transcription in a PRC1-independent fashion, whereas gene repression by the long CBX2-1 isoform is mediated by the PRC1 protein complex. PMID: 22419124
    19. Cells depleted of PRC1 failed to form a polarized microtubule array or ectopic furrows following mitotic exit, and recruitment of Aurora B kinase, male germ cell Rac GTPase-activating protein, and RhoA to the cortex was impaired. PMID: 22323288
    20. The radiosensitizing effect of paclitaxel on KB cells may be due to the down-regulated expression of PRC1 and cyclin B2. PMID: 19664331
    21. PRC1's microtubule binding is mediated by a structured domain with a spectrin-fold and an unstructured Lys/Arg-rich domain. PMID: 20691902
    22. PRC1 is a microtubule-associated protein required to maintain the spindle midzone, and that distinct functions are associated with modular elements of the primary sequence. PMID: 12082078
    23. contributes to the correct formation of the spindle during the metaphase PMID: 14744859
    24. kinesin family member 4 and its binding partner PRC1 play essential roles in the organization of central spindles and midzone formation PMID: 15297875
    25. transcription is controlled by p53 via regulating cytokinesis PMID: 15531928
    26. PRC1 is a microtubule bundling protein that is critical to the formation of the central spindle, which is required for abscission but not for furrowing in mammalian cells PMID: 15616196
    27. role of PRC1 in midzone formation, indicate that cell cycle-dependent translocation of PRC1 by Kif4 is essential for midzone formation and cytokinesis. PMID: 15625105
    28. PRC1 is an essential factor in controlling the spatiotemporal formation of the midzone in human cells. PMID: 16603632
    29. suggest the involvement of a PRC1- kinesin family member 2C/mitotic centromere-associated kinesin complex in breast tumorigenesis, and this complex should be a promising target for the development of novel treatments for breast cancer PMID: 17233835
    30. These results provide a potential mechanistic basis for the defective cytokinesis phenotype exhibited by HSF2-/- cells, as well as suggest a potential role for PRC1 in HSF2-mediated gene bookmarking. PMID: 18570919
    31. Microtubule-associated protein (MAP) Prc1 facilitate Plk1 phosphorylation of HsCyk-4. PMID: 19468300
    32. Data propose that PRC1 forms a link between stabilization of CLASP1 association with central spindle microtubules and anti-parallel microtubule elongation. PMID: 19561070

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  • 亚细胞定位：
    Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, spindle pole. Midbody. Chromosome.
  • 蛋白家族：
    MAP65/ASE1 family
  • 组织特异性：
    Overexpressed in bladder cancer cells.
  • 数据库链接：

    HGNC: 9341

    OMIM: 603484

    KEGG: hsa:9055

    STRING: 9606.ENSP00000377793

    UniGene: Hs.366401