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  4. Recombinant Human Pulmonary surfactant-associated protein A1 (SFTPA1)

Recombinant Human Pulmonary surfactant-associated protein A1 (SFTPA1)

  • 货号：
    CSB-YP810281HU
  • 规格：
    ￥2208
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP810281HU could indicate that this peptide derived from Yeast-expressed Homo sapiens (Human) SFTPA1.
    • Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP810281HU could indicate that this peptide derived from Yeast-expressed Homo sapiens (Human) SFTPA1.
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
    SFTPA1
  • Uniprot No.：
  • 别名：
    SFTPA1; COLEC4; PSAP; SFTP1; SFTPA; SFTPA1B; Pulmonary surfactant-associated protein A1; PSP-A; PSPA; SP-A; SP-A1; 35 kDa pulmonary surfactant-associated protein; Alveolar proteinosis protein; Collectin-4
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    Yeast
  • 分子量：
    26.2 kDa
  • 表达区域：
    21-248aa
  • 氨基酸序列
    EVKDVCVGSPGIPGTPGSHGLPGRDGRDGLKGDPGPPGPMGPPGEMPCPPGNDGLPGAPGIPGECGEKGEPGERGPPGLPAHLDEELQATLHDFRHQILQTRGALSLQGSIMTVGEKVFSSNGQSITFDAIQEACARAGGRIAVPRNPEENEAIASFVKKYNTYAYVGLTEGPSPGDFRYSDGTPVNYTNWYRGEPAGRGKEQCVEMYTDGQWNDRNCLYSRLTICEF
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

问答及客户评论

■ 常见问题解答
Q:

I am looking for recombinant human surfactant protein A, he needs an active form for cell line. Can you offer us any protein in active form?

A:
Thanks for your inquiry.
Recombinant Human Pulmonary surfactant-associated protein A1(SFTPA1)
CSB-YP810281HU >> Yeast
CSB-BP810281HU >> Baculovirus
CSB-MP810281HU >> Mammalian cell
Express Region:21-248aa; Full length mature protein.
Tag information:N-terminal 6xHis-tagged
Expression Sequence:

EVKDVCVGSPGIPGTPGSHGLPGRDGRDGLKGDPGPPGPMGPPGEMPCPPGNDGLPGAPGIPGECGEKGEPGERGPPGLPAHLDEELQATLHDFRHQILQTRGALSLQGSIMTVGEKVFSSNGQSITFDAIQEACARAGGRIAVPRNPEENEAIASFVKKYNTYAYVGLTEGPSPGDFRYSDGTPVNYTNWYRGEPAGRGKEQCVEMYTDGQWNDRNCLYSRLTICEF


As to the activity, we have not detected it yet, so we can't gurantee.

靶点详情

  • 功能：
    In presence of calcium ions, it binds to surfactant phospholipids and contributes to lower the surface tension at the air-liquid interface in the alveoli of the mammalian lung and is essential for normal respiration. Enhances the expression of MYO18A/SP-R210 on alveolar macrophages.; (Microbial infection) Recognition of M.tuberculosis by dendritic cells may occur partially via this molecule. Can recognize, bind, and opsonize pathogens to enhance their elimination by alveolar macrophages.
  • 基因功能参考文献：
    1. This study is suggestive that the PCR (polymerase chain reaction) for the detection of coa, mecA, and spa gene is a fast, accurate, and valuable diagnostic tool. PMID: 30074012
    2. TGF beta markedly decreased expression of SP-A, SP-B, SP-C, fatty acid synthase, and the phospholipid transporter ABCA3. However, TGF beta increased protein levels of SP-D with little change in mRNA levels. PMID: 29621540
    3. this study shows that concentrations of SP-A are associated with polarization of macrophages in pleural effusions of non-small cell lung cancer PMID: 29111316
    4. the SPA and SPD levels in EBC were correlated with lung function, which contributed to COPD diagnosis. PMID: 28791362
    5. Data (including data from studies using transgenic/knockout mice) suggest that surfactant protein A1 (SPA1) interferes with EGF binding to EGFR in pulmonary alveoli cell lines; SPA1 directly binds extracellular domain of EGFR; binding of SPA1 to EGFR appears to be different from binding of SPD to EGFR; binding of SPA1 to EGFR does not suppress EGF-induced phosphorylation of EGFR or cell proliferation. PMID: 28972165
    6. This review intends to provide a current overview of the genetics, structure and extra-pulmonary functions of the surfactant collectin proteins. PMID: 28351530
    7. The SFTPA1 haplotype comprising rs17881720-A and rs17879335-G was a resistance factor while the haplotype comprising rs1914663-T and rs1059225-G was found to be a susceptibility factor to tuberculosis in Han Chinese population. PMID: 27012150
    8. SP-A transiently enhances the basal protein expression of CHC and alpha-adaptin. PMID: 26771574
    9. the presence of SP-A1 allows pulmonary surfactant to adopt a particularly favorable structure with optimal biophysical properties PMID: 27508436
    10. SP-A1 6A4 and SP-A2 1A5 genetic variants may influence the susceptibility to respiratory distress syndrome in late-preterm infants, independently of the effect of other perinatal factors. PMID: 27835691
    11. Direct interaction between SP-A or SAP01 and human BD-3 seemed to be responsible for the inhibitory effects on chemotaxis of mast cells. PMID: 28188794
    12. SP-A (+186A/G) and SP-B (1580C/T) polymorphisms are strongly associated with the risk of respiratory distress syndrome (RDS) in preterm infants. Notably, reduced serum SP-A levels were correlated with a high risk of RDS and may serve as novel biomarkers for RDS detection and monitoring. PMID: 28011976
    13. Surfactant protein A levels differed among idiopathic pulmonary fibrosis, pulmonary sarcoidosis and chronic pulmonary obstructive disease. PMID: 27758987
    14. Differences were found regarding SPA and pro-SPB expression in the vast majority of subjects: in some lungs, SPA was more expressed whereas in others pro-SPB showed an higher degree of immunoreactivity. The expression of both surfactant proteins was not strictly correlated with gestational age. PMID: 27734990
    15. Study identifies SFTPA1 as a gene involved in familial idiopathic interstitial pneumonia and shows that altered SP-A1 expression is associated with a large spectrum of phenotypic pulmonary manifestations ranging from severe respiratory insufficiency occurring early in life to lung fibrosis and cancer in adult patients. PMID: 26792177
    16. These results suggested that the measurement of SPA levels in the exhaled breath condensate may serve as a method for monitoring airway obstruction in patients with chronic obstructive pulmonary disease. PMID: 26707652
    17. Human and murine data together indicate that SP-A, SP-D and MBL are synthesized in early gestational tissues, and may contribute to regulation of immune response at the feto-maternal interface during pregnancy. PMID: 26603976
    18. SP-A in exhaled particles from small airways may represent a promising non-invasive biomarker of disease in chronic obstructive pulmonary disease patients. PMID: 26656890
    19. In this study, the loci and haplotypes associated with pulmonary tuberculosis (PTB)were found mostly to be located in the SFTPA2 gene, suggesting that the effects of the SFTPA2 gene on PTB are stronger than those of SFTPA1. PMID: 24984162
    20. Data suggest that that surfactant protein A(SP-A) levels may be important to maintain surfactant function in the presence of high cholesterol within surfactant. PMID: 25522687
    21. SP-A modulates lung immune system in the area of non-infectious lung diseases such as acute and chronic pulmonary fibrosis and lung cancer. PMID: 24705741
    22. 12 of which predicted to bind SP-A 3'UTRs. PMID: 25058539
    23. This study shows that changes occur in the alveolar macrophage proteome in response to a single in vivo treatment with exogenous SP-A1 and/or SP-A2. PMID: 24954098
    24. SP-A1 transcripts, which in turn negatively affect SP-A1 translation, possibly affecting SP-A1/SP-A2 ratio, with potential for clinical implication. PMID: 25326576
    25. In this review, we highlight the associations of eosinophilic lung diseases with SP-A and SP-D levels and functions. PMID: 24960334
    26. sequence variability at the 3'UTR of SFTPA1 and SFTPA2 gene variants differentially affects miRNA regulation of gene expression. PMID: 24793167
    27. Results show that cloned surfactant-associated protein A (SPA) gene promoter had specific transcriptional activity in SPA high expression cells. PMID: 24939295
    28. The result suggested that polymorphism of aa62 (CCA/CCG, rs1136451) of SP-A may be associated with the susceptibility to COPD in Xinjiang Uighurs. PMID: 22528218
    29. The current meta-analysis indicates that common polymorphisms in the SP-A gene may contribute to increasing susceptibility to COPD, especially among Asian populations. PMID: 24093802
    30. SP-A gene polymorphism is associated with kidney graft rejection. PMID: 24007361
    31. SP-A inhibited lung cancer progression by recruiting and activating natural killer cells via controlling the polarization of tumor-associated macrophages. PMID: 23499372
    32. The obtained results suggested that in vitro approach can be used to study pulmonary toxicity as long as the applied in vitro model mimics closely the complexity of in vivo situation. PMID: 22769972
    33. Cat S may participate in the pathophysiology of cystic fibrosis by weakening the antibacterial activity of SP-A. PMID: 23707200
    34. The aim of this report is to describe the genetic complexity of the SFTPA1 and SFTPA2 genes, as well as to review regulatory mechanisms that control SP-A expression in humans and other animal species.[review] PMID: 23069847
    35. elevated expression in nasal mucosa in chronic rhinosinusitis with polyps PMID: 23406594
    36. Surfactant Protein-A, like other host defense molecules in the reproductive tract, appears to be regulated by gonadal hormones. PMID: 22672628
    37. Surfactant protein A associated with respiratory distress syndrome in Korean preterm infants: evidence of ethnic difference. PMID: 23038062
    38. Lipoteichoic acid can increase SP-A synthesis in human alveolar type II epithelial cells through sequentially activating the MEK1-ERK1/2-NF-kappaB-dependent pathway PMID: 23031213
    39. Gln238Lys missense variant of exon 6 in the SFTPA1 may have potential susceptibility in the development of IPF, which was shown in patients with sporadic IPF with a statistically higher frequency. PMID: 22884059
    40. Surfactant protein-A suppresses eosinophil-mediated killing of Mycoplasma pneumoniae in allergic lungs PMID: 22384248
    41. review focuses on published association studies of surfactant proteins A and D genetic polymorphisms with respiratory, and non-respiratory diseases in adults, children, and newborns [review] PMID: 22201752
    42. SP-A protects lung epithelium from tissue injury caused by BD3 PMID: 22418431
    43. Surfactant protein A is defective in abrogating inflammation in asthma. PMID: 21784968
    44. genetic variants of the surfactant protein A1 confer a protective phenotype against severe acute respiratory syncytial infection PMID: 21601013
    45. Both nasal polyps and nasal mucosa expressed SP-A mRNA and protein, but the expression was stronger in nasal polyps. PMID: 20873544
    46. The expression of SP-A in allergic rhinitis and nasal polyps was dramatically higher than that in controls. PMID: 19894557
    47. findings suggest that Suv39H1 and Suv39H2 are key hypoxia-induced methyltransferases; their decline in fetal lung during late gestation is critical for epigenetic changes resulting in the developmental induction of SP-A PMID: 21402781
    48. Paucity of immunohistochemical SP-A expression in alveolar epithelial cells in diseased areas may predict a worse prognosis for patients with idiopathic interstitial pneumonias. PMID: 21435274
    49. High SPA-1 is associated with invasion and metastasis of prostate cancer. PMID: 21251160
    50. SP-A likely plays a critical role in regulating prostaglandin production within the uterus, culminating at term in decidual activation and the onset of labor. PMID: 21270323

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  • 相关疾病：
    Pulmonary fibrosis, idiopathic (IPF); Respiratory distress syndrome in premature infants (RDS)
  • 亚细胞定位：
    Secreted, extracellular space, extracellular matrix. Secreted, extracellular space, surface film.
  • 蛋白家族：
    SFTPA family
  • 数据库链接：

    HGNC: 10798

    OMIM: 178500

    KEGG: hsa:653509

    STRING: 9606.ENSP00000397082

    UniGene: Hs.535295