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  4. Recombinant Human Putative methyltransferase NSUN3 (NSUN3)

Recombinant Human Putative methyltransferase NSUN3 (NSUN3)

  • 中文名称：
    人NSUN3重组蛋白
  • 货号：
    CSB-YP867156HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人NSUN3重组蛋白
  • 货号：
    CSB-EP867156HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人NSUN3重组蛋白
  • 货号：
    CSB-EP867156HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人NSUN3重组蛋白
  • 货号：
    CSB-BP867156HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人NSUN3重组蛋白
  • 货号：
    CSB-MP867156HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    NSUN3
  • Uniprot No.：
  • 别名：
    6720484A09Rik; AU022521; FLJ22109; FLJ22609; MST077; MSTP077; NOL1/NOP2/Sun domain family 3; NOL1/NOP2/Sun domain family member 3; NOP2/Sun domain family member 3; NSUN3; NSUN3_HUMAN; OTTMUSP00000028633; Putative methyltransferase NSUN3; UG0651E06
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-340
  • 氨基酸序列
    MLTQLKAKSE GKLAKQICKV VLDHFEKQYS KELGDAWNTV REILTSPSCW QYAVLLNRFN YPFELEKDLH LKGYHTLSQG SLPNYPKSVK CYLSRTPGRI PSERHQIGNL KKYYLLNAAS LLPVLALELR DGEKVLDLCA APGGKSIALL QCACPGYLHC NEYDSLRLRW LRQTLESFIP QPLINVIKVS ELDGRKMGDA QPEMFDKVLV DAPCSNDRSW LFSSDSQKAS CRISQRRNLP LLQIELLRSA IKALRPGGIL VYSTCTLSKA ENQDVISEIL NSHGNIMPMD IKGIARTCSH DFTFAPTGQE CGLLVIPDKG KAWGPMYVAK LKKSWSTGKW
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Mitochondrial tRNA methyltransferase that mediates methylation of cytosine to 5-methylcytosine (m5C) at position 34 of mt-tRNA(Met). mt-tRNA(Met) methylation at cytosine(34) takes place at the wobble position of the anticodon and initiates the formation of 5-formylcytosine (f(5)c) at this position. mt-tRNA(Met) containing the f(5)c modification at the wobble position enables recognition of the AUA codon in addition to the AUG codon, expanding codon recognition in mitochondrial translation.
  • 基因功能参考文献：
    1. The s show that the RNA methyltransferase NSUN3 localises to mitochondria and interacts with mt-tRNA(M)(et) to methylate cytosine 34 (C34) at the wobble position. PMID: 27497299
    2. The NSUN3-knockout cells showed strong reduction in mitochondrial protein synthesis and reduced oxygen consumption, leading to deficient mitochondrial activity. It reconstituted formation of 5-methylcytidine (m(5)C) at position 34 (m(5)C34) on mt-tRNA(Met) with recombinant NSUN3 in the presence of AdoMet, demonstrating that NSUN3-mediated m(5)C34 formation initiates f(5)C34 biogenesis. PMID: 27214402
  • 相关疾病：
    Mitochondrial complex deficiency (PubMed:27356879). A disorder of the mitochondrial respiratory chain resulting in a highly variable phenotype depending on which tissues are affected (PubMed:27356879). Clinical features include combined developmental disability, microcephaly, failure to thrive, recurrent increased lactate levels in plasma, muscular weakness, proximal accentuated, external ophthalmoplegia and convergence nystagmus (PubMed:27356879). Defects are probably caused by deficient methylation and formylation of mt-tRNA(Met) wobble cytosine (PubMed:27356879). The disease may be caused by mutations affecting the gene represented in this entry.
  • 亚细胞定位：
    Mitochondrion matrix.
  • 蛋白家族：
    Class I-like SAM-binding methyltransferase superfamily, RsmB/NOP family
  • 数据库链接：

    HGNC: 26208

    OMIM: 617491

    KEGG: hsa:63899

    STRING: 9606.ENSP00000318986

    UniGene: Hs.436594