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  4. Recombinant Human RING finger protein 169 (RNF169)

Recombinant Human RING finger protein 169 (RNF169)

  • 货号：
    CSB-YP019855HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP019855HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP019855HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP019855HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP019855HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    RNF169
  • Uniprot No.：
  • 别名：
    E3 ubiquitin-protein ligase RNF169; KIAA1991; RING finger protein 169; RN169_HUMAN; RNF169
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-708
  • 氨基酸序列
    MAAAGPSTRA SSAAAAAALS RRGRRGRCDE TAAAKTGAPG PASGPSLLVL SPPLLQPPLP PRPEESGCAG CLEPPGEAAA LPCGHSLCRG CAQRAADAAG PGCPRCRARG PGWARRRARD DGQADSEVLG ECARRSQPER CRPRRDGGAA AAGPRPEQEP RAAPAEPDFI FRAPIKLSKP GELREEYESL RKLREEKLQE EKPSEDQIHK LLPEDTETGK RKMDEQKKRD EPLVLKTNLE RCPARLSDSE NEEPSRGQMT QTHRSAFVSK NNSYSLAFLA GKLNSKVERS QSCSDTAQER AKSRVRAVPG NKAKVTTMTP ASNPIIGVLL STQNNRCVSA PDLTIEKRLP FSSLSSLASL HKPERSVSPE SNDSISEELN HFKPIVCSPC TPPKRLPDGR VLSPLIIKST PRNLNRSLQK QTSYEASPRI LKKWEQIFQE RQIKKTLSKA TLTSLAPEMG EELLGSEGIH SSKEKPLVAV NTRLSGGQVL SEYTGPTSAD LDHFPSVSQT KAEQDSDNKS STEIPLETCC SSELKGGGSG TSLEREQFEG LGSTPDAKLD KTCISRAMKI TTVNSVLPQN SVLGGVLKTK QQLKTLNHFD LTNGVLVESL SEEPLPSLRR GRKRHCKTKH LEQNGSLKKL RQTSGEVGLA PTDPVLREME QKLQQEEEDR QLALQLQRMF DNERRTVSRR KGSVDQYLLR SSNMAGAK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Probable E3 ubiquitin-protein ligase that acts as a negative regulator of double-strand breaks (DSBs) repair following DNA damage. Recruited to DSB repair sites by recognizing and binding ubiquitin catalyzed by RNF168 and competes with TP53BP1 and BRCA1 for association with RNF168-modified chromatin, thereby acting as a negative regulator of DSBs repair. E3 ubiquitin-protein ligase activity is not required for regulation of DSBs repair.
  • 基因功能参考文献：
    1. results highlight the interplay of RNF169 with 53BP1 in fine-tuning choice of DSB repair pathways. PMID: 30104380
    2. USP7 is RNF169 interacting protein.Expression of USP7 and RNF169 positively correlated in breast cancer. PMID: 28325877
    3. The s establish that RNF169 binds to ubiquitylated histone H2A-Lys13/Lys15 in a manner that involves its canonical ubiquitin-binding helix and a pair of arginine-rich motifs that interact with the nucleosome acidic patch. PMID: 28406400
    4. Ubiquitin ligases RNF168, RNF169, and RAD18 specifically bind histone H2A Lys13/15-ubiquitylated nucleosomes. 53BP1 chromatin recruitment may be activated by RNF168 and blocked by RNF169 and RAD18. PMID: 28506460
    5. RNF169 as a component in DNA damage signal transduction and adds to the complexity of regulatory ubiquitylation in genome stability maintenance. PMID: 22733822
    6. RNF168, its paralog RNF169, RAD18, and the BRCA1-interacting RAP80 protein accumulate at DNA double strand break sites through the use of bipartite modules composed of ubiquitin binding domains. PMID: 22742833
    7. results show that RNF169 functions in a noncanonical fashion to harness RNF168-mediated protein recruitment to DSB-containing chromatin, thereby contributing to regulation of DSB repair pathway utilization. PMID: 22492721

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  • 亚细胞定位：
    Nucleus, nucleoplasm. Note=Localizes to sites of double-strand breaks (DSBs) following DNA damage. Recruited to DSBs via recognition of RNF168-dependent ubiquitin products.
  • 蛋白家族：
    RNF169 family
  • 数据库链接：

    HGNC: 26961

    KEGG: hsa:254225

    STRING: 9606.ENSP00000299563

    UniGene: Hs.370145